Occurrence of nitrifiers and diversity of ammonia-oxidizing bacteria in developing drinking water biofilms.

Mari T.T. Lipponen, Pertti J. Martikainen, Ritva E. Vasara, Kristina Servomaa, Outi Zacheus, Merja Kontro

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

We studied the population dynamics of nitrifyingbacteria duringthe development of biofilms up to 233 or 280 days on polyvinylchloride pipes connected to two full-scale drinkingwater distribution networks supplyingprocessed and chloraminated surface water. The numbers of nitrifiers in biofilms were enumerated at intervals of 10–64 days by the most probable number (MPN) method at waterworks and at several study sites in distribution network areas. The numbers of nitrifiers increased towards the distal sites. The highest detected MPN counts of ammonia-oxidizing
bacteria (AOB) for study areas 1 and 7 were 500MPN/cm2 and 1.0 x106 MPN/cm2, and those of nitrite-oxidizing bacteria (NOB) 96MPN/cm2
and 2.2x103 MPN/cm2, respectively. The diversity of AOB was determined by PCR amplifying, cloning and sequencing the partial ammonia monooxygenase (amoA) gene of selected biofilm samples presenting different biofilm ages. The PCR primers used, A189 and A682, also amplified a fragment of particulate methane monooxygenase (pmoA) gene of methane-oxidizing bacteria. The majority of biofilm clones (24 out of 30 studied) contained Nitrosomonas amoA-like sequences. There were only two pmoA-like sequences of Type I methanotrophs, and four sequences positioned in amoA/pmoA sequence groups of uncultured bacteria. From both study area very similar or even completely identical Nitrosomonas amoA-like sequences were obtained despite of high difference in AOB numbers. The results show that the conditions in newly formed biofilms in drinkingwater distribution systems favor the growth of Nitrosomonas-type AOB.
Alkuperäiskielienglanti
LehtiWater Research
Vuosikerta38
Sivut4424-4434
Sivumäärä11
ISSN0043-1354
TilaJulkaistu - 2004
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Lainaa tätä

Lipponen, M. T. T., Martikainen, P. J., Vasara, R. E., Servomaa, K., Zacheus, O., & Kontro, M. (2004). Occurrence of nitrifiers and diversity of ammonia-oxidizing bacteria in developing drinking water biofilms. Water Research, 38, 4424-4434.
Lipponen, Mari T.T. ; Martikainen, Pertti J. ; Vasara, Ritva E. ; Servomaa, Kristina ; Zacheus, Outi ; Kontro, Merja. / Occurrence of nitrifiers and diversity of ammonia-oxidizing bacteria in developing drinking water biofilms. Julkaisussa: Water Research. 2004 ; Vuosikerta 38. Sivut 4424-4434.
@article{b73a416d519843879f2b2f8fc8f82d3e,
title = "Occurrence of nitrifiers and diversity of ammonia-oxidizing bacteria in developing drinking water biofilms.",
abstract = "We studied the population dynamics of nitrifyingbacteria duringthe development of biofilms up to 233 or 280 days on polyvinylchloride pipes connected to two full-scale drinkingwater distribution networks supplyingprocessed and chloraminated surface water. The numbers of nitrifiers in biofilms were enumerated at intervals of 10–64 days by the most probable number (MPN) method at waterworks and at several study sites in distribution network areas. The numbers of nitrifiers increased towards the distal sites. The highest detected MPN counts of ammonia-oxidizingbacteria (AOB) for study areas 1 and 7 were 500MPN/cm2 and 1.0 x106 MPN/cm2, and those of nitrite-oxidizing bacteria (NOB) 96MPN/cm2and 2.2x103 MPN/cm2, respectively. The diversity of AOB was determined by PCR amplifying, cloning and sequencing the partial ammonia monooxygenase (amoA) gene of selected biofilm samples presenting different biofilm ages. The PCR primers used, A189 and A682, also amplified a fragment of particulate methane monooxygenase (pmoA) gene of methane-oxidizing bacteria. The majority of biofilm clones (24 out of 30 studied) contained Nitrosomonas amoA-like sequences. There were only two pmoA-like sequences of Type I methanotrophs, and four sequences positioned in amoA/pmoA sequence groups of uncultured bacteria. From both study area very similar or even completely identical Nitrosomonas amoA-like sequences were obtained despite of high difference in AOB numbers. The results show that the conditions in newly formed biofilms in drinkingwater distribution systems favor the growth of Nitrosomonas-type AOB.",
author = "Lipponen, {Mari T.T.} and Martikainen, {Pertti J.} and Vasara, {Ritva E.} and Kristina Servomaa and Outi Zacheus and Merja Kontro",
year = "2004",
language = "English",
volume = "38",
pages = "4424--4434",
journal = "Water Research",
issn = "0043-1354",
publisher = "Elsevier Scientific Publ. Co",

}

Lipponen, MTT, Martikainen, PJ, Vasara, RE, Servomaa, K, Zacheus, O & Kontro, M 2004, 'Occurrence of nitrifiers and diversity of ammonia-oxidizing bacteria in developing drinking water biofilms.', Water Research, Vuosikerta 38, Sivut 4424-4434.

Occurrence of nitrifiers and diversity of ammonia-oxidizing bacteria in developing drinking water biofilms. / Lipponen, Mari T.T.; Martikainen, Pertti J.; Vasara, Ritva E.; Servomaa, Kristina; Zacheus, Outi; Kontro, Merja.

julkaisussa: Water Research, Vuosikerta 38, 2004, s. 4424-4434.

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - Occurrence of nitrifiers and diversity of ammonia-oxidizing bacteria in developing drinking water biofilms.

AU - Lipponen, Mari T.T.

AU - Martikainen, Pertti J.

AU - Vasara, Ritva E.

AU - Servomaa, Kristina

AU - Zacheus, Outi

AU - Kontro, Merja

PY - 2004

Y1 - 2004

N2 - We studied the population dynamics of nitrifyingbacteria duringthe development of biofilms up to 233 or 280 days on polyvinylchloride pipes connected to two full-scale drinkingwater distribution networks supplyingprocessed and chloraminated surface water. The numbers of nitrifiers in biofilms were enumerated at intervals of 10–64 days by the most probable number (MPN) method at waterworks and at several study sites in distribution network areas. The numbers of nitrifiers increased towards the distal sites. The highest detected MPN counts of ammonia-oxidizingbacteria (AOB) for study areas 1 and 7 were 500MPN/cm2 and 1.0 x106 MPN/cm2, and those of nitrite-oxidizing bacteria (NOB) 96MPN/cm2and 2.2x103 MPN/cm2, respectively. The diversity of AOB was determined by PCR amplifying, cloning and sequencing the partial ammonia monooxygenase (amoA) gene of selected biofilm samples presenting different biofilm ages. The PCR primers used, A189 and A682, also amplified a fragment of particulate methane monooxygenase (pmoA) gene of methane-oxidizing bacteria. The majority of biofilm clones (24 out of 30 studied) contained Nitrosomonas amoA-like sequences. There were only two pmoA-like sequences of Type I methanotrophs, and four sequences positioned in amoA/pmoA sequence groups of uncultured bacteria. From both study area very similar or even completely identical Nitrosomonas amoA-like sequences were obtained despite of high difference in AOB numbers. The results show that the conditions in newly formed biofilms in drinkingwater distribution systems favor the growth of Nitrosomonas-type AOB.

AB - We studied the population dynamics of nitrifyingbacteria duringthe development of biofilms up to 233 or 280 days on polyvinylchloride pipes connected to two full-scale drinkingwater distribution networks supplyingprocessed and chloraminated surface water. The numbers of nitrifiers in biofilms were enumerated at intervals of 10–64 days by the most probable number (MPN) method at waterworks and at several study sites in distribution network areas. The numbers of nitrifiers increased towards the distal sites. The highest detected MPN counts of ammonia-oxidizingbacteria (AOB) for study areas 1 and 7 were 500MPN/cm2 and 1.0 x106 MPN/cm2, and those of nitrite-oxidizing bacteria (NOB) 96MPN/cm2and 2.2x103 MPN/cm2, respectively. The diversity of AOB was determined by PCR amplifying, cloning and sequencing the partial ammonia monooxygenase (amoA) gene of selected biofilm samples presenting different biofilm ages. The PCR primers used, A189 and A682, also amplified a fragment of particulate methane monooxygenase (pmoA) gene of methane-oxidizing bacteria. The majority of biofilm clones (24 out of 30 studied) contained Nitrosomonas amoA-like sequences. There were only two pmoA-like sequences of Type I methanotrophs, and four sequences positioned in amoA/pmoA sequence groups of uncultured bacteria. From both study area very similar or even completely identical Nitrosomonas amoA-like sequences were obtained despite of high difference in AOB numbers. The results show that the conditions in newly formed biofilms in drinkingwater distribution systems favor the growth of Nitrosomonas-type AOB.

M3 - Article

VL - 38

SP - 4424

EP - 4434

JO - Water Research

JF - Water Research

SN - 0043-1354

ER -