"Nicotiana benthamiana was transformed with PI or VPg cistron of Potato virus A (PVA, genus Potyvirus). For both transgenes. T1 progeny displayed (i) resistance to PVA infection, (ii) susceptibility, or (iii) systemic infection followed by recovery of new leaves from PVA infection (RC), regardless ofthe transgene. In RC plants, fully recovered leaves contained no detectable PVA RNA, were highly resistant to challenge inoculation with PVA, and had barely detectable steady-state levels of transgene mRNA-, transgene-homologous siRNA was not detected, in contrast to leaves undergoing recovery. Tops in RC plants and PVA-susceptible transgenic plants were replaced with scions from wild-type plants; only scions on the latter became PVA-infected. These findings suggest that vascular movement of PVA from lower, infected parts of RC plants was compromised in the recovered section expressing RNA silencing-based resistance, which adds a novel dimension to the current models for potyvirus movement. (c) 2005 Elsevier B.V. All rights reserved."
- 414 Maatalouden bioteknologia
- siirtogeeniset kasvit
- 411 Maatalous ja metsätieteet
Germundsson, A., & Valkonen, J. (2006). P1- and VPg-transgenic plants show similar resistance to Potato virus A and may compromise long distance movement of the virus in plant sections expressing RNA silencing-based resistance. Virus Research, 116, 208-213. https://doi.org/10.1016/j.virusres.2005.10.015