Quantification of roots in soil, plant-fungal symbioses and in mixed plant populations with real-time PCR

Ruslan Kalendar, Laura Linnea Vottonen, Mervi Mirjam Seppänen

Tutkimustuotos: KonferenssimateriaalitKonferenssiesitys

Kuvaus

Plant-soil and underground plant-plant interactions have direct implications for the conservation of biodiversity, plant productivity and understanding of local ecosystem responses to global environmental change. Study of associated organisms in the soil, symbiosis and in mixed plant populations, is limited by difficulties in the species quantification and sensitive detection. We have designed and tested new PCR primers and hydrolysis probes for TaqMan assays that target the nuclear ribosomal gene plant DNA. Species-specific probes and universal primers were developed for the monocots and dicots plant species and evaluated for specificity and sensitivity in TaqMan assays on DNA extracted from soil and in mixed plant populations. The assays were sensitive and able to quantify rear targets in mixed plant populations. This approach appears to offer a quick and sensitive way to study roots in soil, symbiosis and in mixed plant populations composition and could provide insights into root function and responses not readily provided by other methods. These features identify the new primer sets developed in this study as useful complements to other universal primers for the study of plant diversity and community composition.
Alkuperäiskielienglanti
Sivut1
Sivumäärä3
TilaJulkaistu - 27 kesäkuuta 2019
OKM-julkaisutyyppiEi sovellu
TapahtumaEGF-EUCARPIA Joint Symposium 2019: “Improving sown grasslands through breeding and management” - ETH Zurich, Switzerland, Zurich, Sveitsi
Kesto: 24 kesäkuuta 201927 kesäkuuta 2019
https://www.egfeucarpia2019.ch/

Konferenssi

KonferenssiEGF-EUCARPIA Joint Symposium 2019
LyhennettäEGF-EUCARPIA
MaaSveitsi
KaupunkiZurich
Ajanjakso24/06/201927/06/2019
www-osoite

Tieteenalat

  • 4111 Maataloustiede

Lainaa tätä

Kalendar, R., Vottonen, L. L., & Seppänen, M. M. (2019). Quantification of roots in soil, plant-fungal symbioses and in mixed plant populations with real-time PCR. 1. Julkaisun esittämispaikka: EGF-EUCARPIA Joint Symposium 2019, Zurich, Sveitsi.
Kalendar, Ruslan ; Vottonen, Laura Linnea ; Seppänen, Mervi Mirjam. / Quantification of roots in soil, plant-fungal symbioses and in mixed plant populations with real-time PCR. Julkaisun esittämispaikka: EGF-EUCARPIA Joint Symposium 2019, Zurich, Sveitsi.3 Sivumäärä
@conference{8d5b9263c71c4984a28e4ff6b06a37ad,
title = "Quantification of roots in soil, plant-fungal symbioses and in mixed plant populations with real-time PCR",
abstract = "Plant-soil and underground plant-plant interactions have direct implications for the conservation of biodiversity, plant productivity and understanding of local ecosystem responses to global environmental change. Study of associated organisms in the soil, symbiosis and in mixed plant populations, is limited by difficulties in the species quantification and sensitive detection. We have designed and tested new PCR primers and hydrolysis probes for TaqMan assays that target the nuclear ribosomal gene plant DNA. Species-specific probes and universal primers were developed for the monocots and dicots plant species and evaluated for specificity and sensitivity in TaqMan assays on DNA extracted from soil and in mixed plant populations. The assays were sensitive and able to quantify rear targets in mixed plant populations. This approach appears to offer a quick and sensitive way to study roots in soil, symbiosis and in mixed plant populations composition and could provide insights into root function and responses not readily provided by other methods. These features identify the new primer sets developed in this study as useful complements to other universal primers for the study of plant diversity and community composition.",
keywords = "4111 Agronomy",
author = "Ruslan Kalendar and Vottonen, {Laura Linnea} and Sepp{\"a}nen, {Mervi Mirjam}",
year = "2019",
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note = "EGF-EUCARPIA Joint Symposium 2019 : “Improving sown grasslands through breeding and management”, EGF-EUCARPIA ; Conference date: 24-06-2019 Through 27-06-2019",
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Kalendar, R, Vottonen, LL & Seppänen, MM 2019, 'Quantification of roots in soil, plant-fungal symbioses and in mixed plant populations with real-time PCR' Artikkeli esitetty EGF-EUCARPIA Joint Symposium 2019, Zurich, Sveitsi, 24/06/2019 - 27/06/2019, Sivut 1.

Quantification of roots in soil, plant-fungal symbioses and in mixed plant populations with real-time PCR. / Kalendar, Ruslan; Vottonen, Laura Linnea; Seppänen, Mervi Mirjam.

2019. 1 Julkaisun esittämispaikka: EGF-EUCARPIA Joint Symposium 2019, Zurich, Sveitsi.

Tutkimustuotos: KonferenssimateriaalitKonferenssiesitys

TY - CONF

T1 - Quantification of roots in soil, plant-fungal symbioses and in mixed plant populations with real-time PCR

AU - Kalendar, Ruslan

AU - Vottonen, Laura Linnea

AU - Seppänen, Mervi Mirjam

PY - 2019/6/27

Y1 - 2019/6/27

N2 - Plant-soil and underground plant-plant interactions have direct implications for the conservation of biodiversity, plant productivity and understanding of local ecosystem responses to global environmental change. Study of associated organisms in the soil, symbiosis and in mixed plant populations, is limited by difficulties in the species quantification and sensitive detection. We have designed and tested new PCR primers and hydrolysis probes for TaqMan assays that target the nuclear ribosomal gene plant DNA. Species-specific probes and universal primers were developed for the monocots and dicots plant species and evaluated for specificity and sensitivity in TaqMan assays on DNA extracted from soil and in mixed plant populations. The assays were sensitive and able to quantify rear targets in mixed plant populations. This approach appears to offer a quick and sensitive way to study roots in soil, symbiosis and in mixed plant populations composition and could provide insights into root function and responses not readily provided by other methods. These features identify the new primer sets developed in this study as useful complements to other universal primers for the study of plant diversity and community composition.

AB - Plant-soil and underground plant-plant interactions have direct implications for the conservation of biodiversity, plant productivity and understanding of local ecosystem responses to global environmental change. Study of associated organisms in the soil, symbiosis and in mixed plant populations, is limited by difficulties in the species quantification and sensitive detection. We have designed and tested new PCR primers and hydrolysis probes for TaqMan assays that target the nuclear ribosomal gene plant DNA. Species-specific probes and universal primers were developed for the monocots and dicots plant species and evaluated for specificity and sensitivity in TaqMan assays on DNA extracted from soil and in mixed plant populations. The assays were sensitive and able to quantify rear targets in mixed plant populations. This approach appears to offer a quick and sensitive way to study roots in soil, symbiosis and in mixed plant populations composition and could provide insights into root function and responses not readily provided by other methods. These features identify the new primer sets developed in this study as useful complements to other universal primers for the study of plant diversity and community composition.

KW - 4111 Agronomy

UR - https://www.egfeucarpia2019.ch/

UR - https://www.europeangrassland.org/en/infos/printed-matter/proceedings.html

M3 - Paper

SP - 1

ER -

Kalendar R, Vottonen LL, Seppänen MM. Quantification of roots in soil, plant-fungal symbioses and in mixed plant populations with real-time PCR. 2019. Julkaisun esittämispaikka: EGF-EUCARPIA Joint Symposium 2019, Zurich, Sveitsi.