Radiolabeled Molecular Imaging Probes for the in Vivo Evaluation of Cellulose Nanocrystals for Biomedical Applications

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

Cellulose nanocrystals (CNCs) have remarkable potential to improve the delivery of diagnostic and therapeutic agents to tumors, however, the in vivo studies on CNC biodistribution are still limited. We developed CNC-based imaging probes for the in vitro and in vivo evaluation using two labeling strategies: site-specific hydrazone linkage to the terminal aldehyde of the CNC and non-site-specific activation using 1,1’-carbonyldiimidazole (CDI). The in vivo behavior of unmodified CNC, DOTA-CNC (ald.), and DOTA-CNC (OH) was investigated in healthy and 4T1 breast cancer mouse models. They displayed good biocompatibility in cell models. Moreover, the biodistribution profile and SPECT/CT imaging confirmed that the accumulation of 111In-labeled DOTA-CNC (ald.) and 111In-DOTA-CNC (OH) was primarily in hepatic, splenic, and pulmonary ducts in accordance with the clearance of nontargeted nanoparticles. The developed CNC imaging probes can be used to obtain information with non-invasive imaging on the behavior in vivo to guide structural optimization for targeted delivery.
Alkuperäiskielienglanti
LehtiBiomacromolecules
Vuosikerta20
Numero2
Sivut674–683
Sivumäärä10
ISSN1525-7797
DOI - pysyväislinkit
TilaJulkaistu - helmikuuta 2019
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 116 Kemia
  • 221 Nanoteknologia
  • 317 Farmasia

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@article{26540ff361ab4ab481a2bdfc6a0a843f,
title = "Radiolabeled Molecular Imaging Probes for the in Vivo Evaluation of Cellulose Nanocrystals for Biomedical Applications",
abstract = "Cellulose nanocrystals (CNCs) have remarkable potential to improve the delivery of diagnostic and therapeutic agents to tumors, however, the in vivo studies on CNC biodistribution are still limited. We developed CNC-based imaging probes for the in vitro and in vivo evaluation using two labeling strategies: site-specific hydrazone linkage to the terminal aldehyde of the CNC and non-site-specific activation using 1,1’-carbonyldiimidazole (CDI). The in vivo behavior of unmodified CNC, DOTA-CNC (ald.), and DOTA-CNC (OH) was investigated in healthy and 4T1 breast cancer mouse models. They displayed good biocompatibility in cell models. Moreover, the biodistribution profile and SPECT/CT imaging confirmed that the accumulation of 111In-labeled DOTA-CNC (ald.) and 111In-DOTA-CNC (OH) was primarily in hepatic, splenic, and pulmonary ducts in accordance with the clearance of nontargeted nanoparticles. The developed CNC imaging probes can be used to obtain information with non-invasive imaging on the behavior in vivo to guide structural optimization for targeted delivery.",
keywords = "ANALOG, CANCER, CHARGE, CYTOTOXICITY, DRUG-DELIVERY, GOLD NANOPARTICLES, SHAPE, SILICA NANOPARTICLES, SURFACE MODIFICATION, VITRO, 116 Chemical sciences, 221 Nano-technology, 317 Pharmacy",
author = "Surachet Imlimthan and Sofia Otaru and Outi Kein{\"a}nen and Alexandra Correia and Kalle Lintinen and {Almeida Santos}, Helder and Anu Airaksinen and Mauri Kostiainen and Mirkka Sarparanta",
year = "2019",
month = "2",
doi = "10.1021/acs.biomac.8b01313",
language = "English",
volume = "20",
pages = "674–683",
journal = "Biomacromolecules",
issn = "1525-7797",
publisher = "American Chemical Society",
number = "2",

}

Radiolabeled Molecular Imaging Probes for the in Vivo Evaluation of Cellulose Nanocrystals for Biomedical Applications. / Imlimthan, Surachet; Otaru, Sofia ; Keinänen, Outi ; Correia, Alexandra ; Lintinen, Kalle ; Almeida Santos, Helder; Airaksinen, Anu; Kostiainen, Mauri; Sarparanta, Mirkka .

julkaisussa: Biomacromolecules, Vuosikerta 20, Nro 2, 02.2019, s. 674–683.

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - Radiolabeled Molecular Imaging Probes for the in Vivo Evaluation of Cellulose Nanocrystals for Biomedical Applications

AU - Imlimthan, Surachet

AU - Otaru, Sofia

AU - Keinänen, Outi

AU - Correia, Alexandra

AU - Lintinen, Kalle

AU - Almeida Santos, Helder

AU - Airaksinen, Anu

AU - Kostiainen, Mauri

AU - Sarparanta, Mirkka

PY - 2019/2

Y1 - 2019/2

N2 - Cellulose nanocrystals (CNCs) have remarkable potential to improve the delivery of diagnostic and therapeutic agents to tumors, however, the in vivo studies on CNC biodistribution are still limited. We developed CNC-based imaging probes for the in vitro and in vivo evaluation using two labeling strategies: site-specific hydrazone linkage to the terminal aldehyde of the CNC and non-site-specific activation using 1,1’-carbonyldiimidazole (CDI). The in vivo behavior of unmodified CNC, DOTA-CNC (ald.), and DOTA-CNC (OH) was investigated in healthy and 4T1 breast cancer mouse models. They displayed good biocompatibility in cell models. Moreover, the biodistribution profile and SPECT/CT imaging confirmed that the accumulation of 111In-labeled DOTA-CNC (ald.) and 111In-DOTA-CNC (OH) was primarily in hepatic, splenic, and pulmonary ducts in accordance with the clearance of nontargeted nanoparticles. The developed CNC imaging probes can be used to obtain information with non-invasive imaging on the behavior in vivo to guide structural optimization for targeted delivery.

AB - Cellulose nanocrystals (CNCs) have remarkable potential to improve the delivery of diagnostic and therapeutic agents to tumors, however, the in vivo studies on CNC biodistribution are still limited. We developed CNC-based imaging probes for the in vitro and in vivo evaluation using two labeling strategies: site-specific hydrazone linkage to the terminal aldehyde of the CNC and non-site-specific activation using 1,1’-carbonyldiimidazole (CDI). The in vivo behavior of unmodified CNC, DOTA-CNC (ald.), and DOTA-CNC (OH) was investigated in healthy and 4T1 breast cancer mouse models. They displayed good biocompatibility in cell models. Moreover, the biodistribution profile and SPECT/CT imaging confirmed that the accumulation of 111In-labeled DOTA-CNC (ald.) and 111In-DOTA-CNC (OH) was primarily in hepatic, splenic, and pulmonary ducts in accordance with the clearance of nontargeted nanoparticles. The developed CNC imaging probes can be used to obtain information with non-invasive imaging on the behavior in vivo to guide structural optimization for targeted delivery.

KW - ANALOG

KW - CANCER

KW - CHARGE

KW - CYTOTOXICITY

KW - DRUG-DELIVERY

KW - GOLD NANOPARTICLES

KW - SHAPE

KW - SILICA NANOPARTICLES

KW - SURFACE MODIFICATION

KW - VITRO

KW - 116 Chemical sciences

KW - 221 Nano-technology

KW - 317 Pharmacy

U2 - 10.1021/acs.biomac.8b01313

DO - 10.1021/acs.biomac.8b01313

M3 - Article

VL - 20

SP - 674

EP - 683

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 2

ER -