Kuvaus
| Alkuperäiskieli | englanti |
|---|---|
| Lehti | Biomacromolecules |
| Vuosikerta | 20 |
| Numero | 2 |
| Sivut | 674–683 |
| Sivumäärä | 10 |
| ISSN | 1525-7797 |
| DOI - pysyväislinkit | |
| Tila | Julkaistu - helmikuuta 2019 |
| OKM-julkaisutyyppi | A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu |
Tieteenalat
- 116 Kemia
- 221 Nanoteknologia
- 317 Farmasia
Lainaa tätä
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Radiolabeled Molecular Imaging Probes for the in Vivo Evaluation of Cellulose Nanocrystals for Biomedical Applications. / Imlimthan, Surachet; Otaru, Sofia ; Keinänen, Outi ; Correia, Alexandra ; Lintinen, Kalle ; Almeida Santos, Helder; Airaksinen, Anu; Kostiainen, Mauri; Sarparanta, Mirkka .
julkaisussa: Biomacromolecules, Vuosikerta 20, Nro 2, 02.2019, s. 674–683.Tutkimustuotos: Artikkelijulkaisu › Artikkeli › Tieteellinen › vertaisarvioitu
TY - JOUR
T1 - Radiolabeled Molecular Imaging Probes for the in Vivo Evaluation of Cellulose Nanocrystals for Biomedical Applications
AU - Imlimthan, Surachet
AU - Otaru, Sofia
AU - Keinänen, Outi
AU - Correia, Alexandra
AU - Lintinen, Kalle
AU - Almeida Santos, Helder
AU - Airaksinen, Anu
AU - Kostiainen, Mauri
AU - Sarparanta, Mirkka
PY - 2019/2
Y1 - 2019/2
N2 - Cellulose nanocrystals (CNCs) have remarkable potential to improve the delivery of diagnostic and therapeutic agents to tumors, however, the in vivo studies on CNC biodistribution are still limited. We developed CNC-based imaging probes for the in vitro and in vivo evaluation using two labeling strategies: site-specific hydrazone linkage to the terminal aldehyde of the CNC and non-site-specific activation using 1,1’-carbonyldiimidazole (CDI). The in vivo behavior of unmodified CNC, DOTA-CNC (ald.), and DOTA-CNC (OH) was investigated in healthy and 4T1 breast cancer mouse models. They displayed good biocompatibility in cell models. Moreover, the biodistribution profile and SPECT/CT imaging confirmed that the accumulation of 111In-labeled DOTA-CNC (ald.) and 111In-DOTA-CNC (OH) was primarily in hepatic, splenic, and pulmonary ducts in accordance with the clearance of nontargeted nanoparticles. The developed CNC imaging probes can be used to obtain information with non-invasive imaging on the behavior in vivo to guide structural optimization for targeted delivery.
AB - Cellulose nanocrystals (CNCs) have remarkable potential to improve the delivery of diagnostic and therapeutic agents to tumors, however, the in vivo studies on CNC biodistribution are still limited. We developed CNC-based imaging probes for the in vitro and in vivo evaluation using two labeling strategies: site-specific hydrazone linkage to the terminal aldehyde of the CNC and non-site-specific activation using 1,1’-carbonyldiimidazole (CDI). The in vivo behavior of unmodified CNC, DOTA-CNC (ald.), and DOTA-CNC (OH) was investigated in healthy and 4T1 breast cancer mouse models. They displayed good biocompatibility in cell models. Moreover, the biodistribution profile and SPECT/CT imaging confirmed that the accumulation of 111In-labeled DOTA-CNC (ald.) and 111In-DOTA-CNC (OH) was primarily in hepatic, splenic, and pulmonary ducts in accordance with the clearance of nontargeted nanoparticles. The developed CNC imaging probes can be used to obtain information with non-invasive imaging on the behavior in vivo to guide structural optimization for targeted delivery.
KW - ANALOG
KW - CANCER
KW - CHARGE
KW - CYTOTOXICITY
KW - DRUG-DELIVERY
KW - GOLD NANOPARTICLES
KW - SHAPE
KW - SILICA NANOPARTICLES
KW - SURFACE MODIFICATION
KW - VITRO
KW - 116 Chemical sciences
KW - 221 Nano-technology
KW - 317 Pharmacy
U2 - 10.1021/acs.biomac.8b01313
DO - 10.1021/acs.biomac.8b01313
M3 - Article
VL - 20
SP - 674
EP - 683
JO - Biomacromolecules
JF - Biomacromolecules
SN - 1525-7797
IS - 2
ER -