Requirement of ecological replication with independent parallel analysis of each replicate plot to support soil remediation

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

Consequences of pseudoreplication or the sole use of plot averages even within one ecosystem on the reliability of laboratory remediation tests have barely been studied even though they may mask field variability. Soils from three field plots were tested separately to reveal the consequences of inadequate use of replicates. Parallel remediation experiments consisting of treatments control (non-contaminated soil), natural attenuation (gasoline-contaminated soil) and biostimulation (gasoline-contaminated and methylene urea fertilized soil) were
conducted to each plot soil. Three destructive sampling days and three pseudoreplicates per treatment, per plot and per sampling day were designed. The concentrations of total and individual gasoline aromatics were followed
using both within-plot pseudoreplicates and plot averages. Results showed that natural attenuation of total or individual gasoline aromatics depended on field plots and that fertilization showed enhancing, inhibiting and negligible effects, respectively, also depending on field plots. Additionally, plot averages masked withintreatment variation between field plots. Conclusively, within-plot pseudoreplication using a single field plot or interpretations based solely on plot averages cannot reveal remediation patchiness observed in practice. Due to
the high soil heterogeneity, ecological between-plot replication together with the parallel analysis of each replicate is encouraged in laboratory studies aiming to design in situ remediation strategies.
Alkuperäiskielienglanti
LehtiInternational Biodeterioration and Biodegradation
Vuosikerta133
Sivut133-141
Sivumäärä9
ISSN0964-8305
DOI - pysyväislinkit
TilaJulkaistu - 2018
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 1183 Kasvibiologia, mikrobiologia, virologia
  • 1172 Ympäristötiede
  • 1181 Ekologia, evoluutiobiologia

Lainaa tätä

@article{aff656085a9e4f49b2a8df8dfffcb197,
title = "Requirement of ecological replication with independent parallel analysis of each replicate plot to support soil remediation",
abstract = "Consequences of pseudoreplication or the sole use of plot averages even within one ecosystem on the reliability of laboratory remediation tests have barely been studied even though they may mask field variability. Soils from three field plots were tested separately to reveal the consequences of inadequate use of replicates. Parallel remediation experiments consisting of treatments control (non-contaminated soil), natural attenuation (gasoline -contaminated soil) and biostimulation (gasoline-contaminated and methylene urea fertilized soil) were conducted to each plot soil. Three destructive sampling days and three pseudoreplicates per treatment, per plot and per sampling day were designed. The concentrations of total and individual gasoline aromatics were followed using both within-plot pseudoreplicates and plot averages. Results showed that natural attenuation of total or individual gasoline aromatics depended on field plots and that fertilization showed enhancing, inhibiting and negligible effects, respectively, also depending on field plots. Additionally, plot averages masked within treatment variation between field plots. Conclusively, within-plot pseudoreplication using a single field plot or interpretations based solely on plot averages cannot reveal remediation patchiness observed in practice. Due to the high soil heterogeneity, ecological between-plot replication together with the parallel analysis of each replicate is encouraged in laboratory studies aiming to design in situ remediation strategies.",
keywords = "1183 Plant biology, microbiology, virology, 1172 Environmental sciences, 1181 Ecology, evolutionary biology, Ecological replication, Pseudoreplication, Soil heterogeneity, Bioremediation, Natural attenuation, Biostimulation, Gasoline contaminatio n, Naphthalene",
author = "Yan Sun and Anna-Lea Rantalainen and Romantschuk, {Martin L.} and Aki Sinkkonen",
year = "2018",
doi = "10.1016/j.ibiod.2018.06.006",
language = "English",
volume = "133",
pages = "133--141",
journal = "International Biodeterioration and Biodegradation",
issn = "0964-8305",
publisher = "Elsevier Scientific Publ. Co",

}

TY - JOUR

T1 - Requirement of ecological replication with independent parallel analysis of each replicate plot to support soil remediation

AU - Sun, Yan

AU - Rantalainen, Anna-Lea

AU - Romantschuk, Martin L.

AU - Sinkkonen, Aki

PY - 2018

Y1 - 2018

N2 - Consequences of pseudoreplication or the sole use of plot averages even within one ecosystem on the reliability of laboratory remediation tests have barely been studied even though they may mask field variability. Soils from three field plots were tested separately to reveal the consequences of inadequate use of replicates. Parallel remediation experiments consisting of treatments control (non-contaminated soil), natural attenuation (gasoline -contaminated soil) and biostimulation (gasoline-contaminated and methylene urea fertilized soil) were conducted to each plot soil. Three destructive sampling days and three pseudoreplicates per treatment, per plot and per sampling day were designed. The concentrations of total and individual gasoline aromatics were followed using both within-plot pseudoreplicates and plot averages. Results showed that natural attenuation of total or individual gasoline aromatics depended on field plots and that fertilization showed enhancing, inhibiting and negligible effects, respectively, also depending on field plots. Additionally, plot averages masked within treatment variation between field plots. Conclusively, within-plot pseudoreplication using a single field plot or interpretations based solely on plot averages cannot reveal remediation patchiness observed in practice. Due to the high soil heterogeneity, ecological between-plot replication together with the parallel analysis of each replicate is encouraged in laboratory studies aiming to design in situ remediation strategies.

AB - Consequences of pseudoreplication or the sole use of plot averages even within one ecosystem on the reliability of laboratory remediation tests have barely been studied even though they may mask field variability. Soils from three field plots were tested separately to reveal the consequences of inadequate use of replicates. Parallel remediation experiments consisting of treatments control (non-contaminated soil), natural attenuation (gasoline -contaminated soil) and biostimulation (gasoline-contaminated and methylene urea fertilized soil) were conducted to each plot soil. Three destructive sampling days and three pseudoreplicates per treatment, per plot and per sampling day were designed. The concentrations of total and individual gasoline aromatics were followed using both within-plot pseudoreplicates and plot averages. Results showed that natural attenuation of total or individual gasoline aromatics depended on field plots and that fertilization showed enhancing, inhibiting and negligible effects, respectively, also depending on field plots. Additionally, plot averages masked within treatment variation between field plots. Conclusively, within-plot pseudoreplication using a single field plot or interpretations based solely on plot averages cannot reveal remediation patchiness observed in practice. Due to the high soil heterogeneity, ecological between-plot replication together with the parallel analysis of each replicate is encouraged in laboratory studies aiming to design in situ remediation strategies.

KW - 1183 Plant biology, microbiology, virology

KW - 1172 Environmental sciences

KW - 1181 Ecology, evolutionary biology

KW - Ecological replication

KW - Pseudoreplication

KW - Soil heterogeneity

KW - Bioremediation

KW - Natural attenuation

KW - Biostimulation

KW - Gasoline contaminatio n

KW - Naphthalene

U2 - 10.1016/j.ibiod.2018.06.006

DO - 10.1016/j.ibiod.2018.06.006

M3 - Article

VL - 133

SP - 133

EP - 141

JO - International Biodeterioration and Biodegradation

JF - International Biodeterioration and Biodegradation

SN - 0964-8305

ER -