Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content

Zachary J Smith, Changwon Lee, Tatu Rojalin, Randy P Carney, Sidhartha Hazari, Alisha Knudson, Kit Lam, Heikki Saari, Elisa Lazaro Ibanez, Tapani Viitala, Timo Laaksonen, Marjo Yliperttula, Sebastian Wachsmann-Hogiu

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

Current analysis of exosomes focuses primarily on bulk analysis, where exosome-to-exosome variability cannot be assessed. In this study, we used Raman spectroscopy to study the chemical composition of single exosomes. We measured spectra of individual exosomes from 8 cell lines. Cell-line-averaged spectra varied considerably, reflecting the variation in total exosomal protein, lipid, genetic, and cytosolic content. Unexpectedly, single exosomes isolated from the same cell type also exhibited high spectral variability. Subsequent spectral analysis revealed clustering of single exosomes into 4 distinct groups that were not cell-line specific. Each group contained exosomes from multiple cell lines, and most cell lines had exosomes in multiple groups. The differences between these groups are related to chemical differences primarily due to differing membrane composition. Through a principal components analysis, we identified that the major sources of spectral variation among the exosomes were in cholesterol content, relative expression of phospholipids to cholesterol, and surface protein expression. For example, exosomes derived from cancerous versus non-cancerous cell lines can be largely separated based on their relative expression of cholesterol and phospholipids. We are the first to indicate that exosome subpopulations are shared among cell types, suggesting distributed exosome functionality. The origins of these differences are likely related to the specific role of extracellular vesicle subpopulations in both normal cell function and carcinogenesis, and they may provide diagnostic potential at the single exosome level.
Alkuperäiskielienglanti
Artikkeli28533
LehtiJournal of Extracellular Vesicles
Vuosikerta4
Sivumäärä15
ISSN2001-3078
DOI - pysyväislinkit
TilaJulkaistu - 7 joulukuuta 2015
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 1182 Biokemia, solu- ja molekyylibiologia
  • 114 Fysiikka

Lainaa tätä

Smith, Z. J., Lee, C., Rojalin, T., Carney, R. P., Hazari, S., Knudson, A., ... Wachsmann-Hogiu, S. (2015). Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content. Journal of Extracellular Vesicles, 4, [28533]. https://doi.org/10.3402/jev.v4.28533
Smith, Zachary J ; Lee, Changwon ; Rojalin, Tatu ; Carney, Randy P ; Hazari, Sidhartha ; Knudson, Alisha ; Lam, Kit ; Saari, Heikki ; Lazaro Ibanez, Elisa ; Viitala, Tapani ; Laaksonen, Timo ; Yliperttula, Marjo ; Wachsmann-Hogiu, Sebastian. / Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content. Julkaisussa: Journal of Extracellular Vesicles. 2015 ; Vuosikerta 4.
@article{dea34227918f45f0bc7e48991122bc5d,
title = "Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content",
abstract = "Current analysis of exosomes focuses primarily on bulk analysis, where exosome-to-exosome variability cannot be assessed. In this study, we used Raman spectroscopy to study the chemical composition of single exosomes. We measured spectra of individual exosomes from 8 cell lines. Cell-line-averaged spectra varied considerably, reflecting the variation in total exosomal protein, lipid, genetic, and cytosolic content. Unexpectedly, single exosomes isolated from the same cell type also exhibited high spectral variability. Subsequent spectral analysis revealed clustering of single exosomes into 4 distinct groups that were not cell-line specific. Each group contained exosomes from multiple cell lines, and most cell lines had exosomes in multiple groups. The differences between these groups are related to chemical differences primarily due to differing membrane composition. Through a principal components analysis, we identified that the major sources of spectral variation among the exosomes were in cholesterol content, relative expression of phospholipids to cholesterol, and surface protein expression. For example, exosomes derived from cancerous versus non-cancerous cell lines can be largely separated based on their relative expression of cholesterol and phospholipids. We are the first to indicate that exosome subpopulations are shared among cell types, suggesting distributed exosome functionality. The origins of these differences are likely related to the specific role of extracellular vesicle subpopulations in both normal cell function and carcinogenesis, and they may provide diagnostic potential at the single exosome level.",
keywords = "1182 Biochemistry, cell and molecular biology, Exosomes, MICROVESICLES, 114 Physical sciences, Raman, Laser trap",
author = "Smith, {Zachary J} and Changwon Lee and Tatu Rojalin and Carney, {Randy P} and Sidhartha Hazari and Alisha Knudson and Kit Lam and Heikki Saari and {Lazaro Ibanez}, Elisa and Tapani Viitala and Timo Laaksonen and Marjo Yliperttula and Sebastian Wachsmann-Hogiu",
year = "2015",
month = "12",
day = "7",
doi = "10.3402/jev.v4.28533",
language = "English",
volume = "4",
journal = "Journal of Extracellular Vesicles",
issn = "2001-3078",
publisher = "Co-Action Publishing",

}

Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content. / Smith, Zachary J; Lee, Changwon; Rojalin, Tatu ; Carney, Randy P; Hazari, Sidhartha; Knudson, Alisha; Lam, Kit; Saari, Heikki ; Lazaro Ibanez, Elisa; Viitala, Tapani ; Laaksonen, Timo ; Yliperttula, Marjo ; Wachsmann-Hogiu, Sebastian.

julkaisussa: Journal of Extracellular Vesicles, Vuosikerta 4, 28533, 07.12.2015.

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content

AU - Smith, Zachary J

AU - Lee, Changwon

AU - Rojalin, Tatu

AU - Carney, Randy P

AU - Hazari, Sidhartha

AU - Knudson, Alisha

AU - Lam, Kit

AU - Saari, Heikki

AU - Lazaro Ibanez, Elisa

AU - Viitala, Tapani

AU - Laaksonen, Timo

AU - Yliperttula, Marjo

AU - Wachsmann-Hogiu, Sebastian

PY - 2015/12/7

Y1 - 2015/12/7

N2 - Current analysis of exosomes focuses primarily on bulk analysis, where exosome-to-exosome variability cannot be assessed. In this study, we used Raman spectroscopy to study the chemical composition of single exosomes. We measured spectra of individual exosomes from 8 cell lines. Cell-line-averaged spectra varied considerably, reflecting the variation in total exosomal protein, lipid, genetic, and cytosolic content. Unexpectedly, single exosomes isolated from the same cell type also exhibited high spectral variability. Subsequent spectral analysis revealed clustering of single exosomes into 4 distinct groups that were not cell-line specific. Each group contained exosomes from multiple cell lines, and most cell lines had exosomes in multiple groups. The differences between these groups are related to chemical differences primarily due to differing membrane composition. Through a principal components analysis, we identified that the major sources of spectral variation among the exosomes were in cholesterol content, relative expression of phospholipids to cholesterol, and surface protein expression. For example, exosomes derived from cancerous versus non-cancerous cell lines can be largely separated based on their relative expression of cholesterol and phospholipids. We are the first to indicate that exosome subpopulations are shared among cell types, suggesting distributed exosome functionality. The origins of these differences are likely related to the specific role of extracellular vesicle subpopulations in both normal cell function and carcinogenesis, and they may provide diagnostic potential at the single exosome level.

AB - Current analysis of exosomes focuses primarily on bulk analysis, where exosome-to-exosome variability cannot be assessed. In this study, we used Raman spectroscopy to study the chemical composition of single exosomes. We measured spectra of individual exosomes from 8 cell lines. Cell-line-averaged spectra varied considerably, reflecting the variation in total exosomal protein, lipid, genetic, and cytosolic content. Unexpectedly, single exosomes isolated from the same cell type also exhibited high spectral variability. Subsequent spectral analysis revealed clustering of single exosomes into 4 distinct groups that were not cell-line specific. Each group contained exosomes from multiple cell lines, and most cell lines had exosomes in multiple groups. The differences between these groups are related to chemical differences primarily due to differing membrane composition. Through a principal components analysis, we identified that the major sources of spectral variation among the exosomes were in cholesterol content, relative expression of phospholipids to cholesterol, and surface protein expression. For example, exosomes derived from cancerous versus non-cancerous cell lines can be largely separated based on their relative expression of cholesterol and phospholipids. We are the first to indicate that exosome subpopulations are shared among cell types, suggesting distributed exosome functionality. The origins of these differences are likely related to the specific role of extracellular vesicle subpopulations in both normal cell function and carcinogenesis, and they may provide diagnostic potential at the single exosome level.

KW - 1182 Biochemistry, cell and molecular biology

KW - Exosomes

KW - MICROVESICLES

KW - 114 Physical sciences

KW - Raman

KW - Laser trap

U2 - 10.3402/jev.v4.28533

DO - 10.3402/jev.v4.28533

M3 - Article

VL - 4

JO - Journal of Extracellular Vesicles

JF - Journal of Extracellular Vesicles

SN - 2001-3078

M1 - 28533

ER -