Sphingolipid biosynthesis modulates plasmodesmal ultrastructure and phloem unloading

Dawei Yan, Shri Yadav, Andrea Paterlini, William J Nicolas, Jules D Petit, Lysiane Brocard, Ilya Belevich, Magali S Grison, Anne Vaten, Leila Karami, Sedeer El-Showk, Jung-Youn Lee, Gosia M Murawska, Jenny Mortimer, Michael Knoblauch, Eija Jokitalo, Jonathan E Markham, Emmanuelle M Bayer, Yrjö Helariutta

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

During phloem unloading, multiple cell-to-cell transport events move organic substances to the root meristem. Although the primary unloading event from the sieve elements to the phloem pole pericycle has been characterized to some extent, little is known about post-sieve element unloading. Here, we report a novel gene, PHLOEM UNLOADING MODULATOR (PLM), in the absence of which plasmodesmata-mediated symplastic transport through the phloem pole pericycle-endodermis interface is specifically enhanced. Increased unloading is attributable to a defect in the formation of the endoplasmic reticulum-plasma membrane tethers during plasmodesmal morphogenesis, resulting in the majority of pores lacking a visible cytoplasmic sleeve. PLM encodes a putative enzyme required for the biosynthesis of sphingolipids with very-long-chain fatty acid. Taken together, our results indicate that post-sieve element unloading involves sphingolipid metabolism, which affects plasmodesmal ultrastructure. They also raise the question of how and why plasmodesmata with no cytoplasmic sleeve facilitate molecular trafficking.
Alkuperäiskielienglanti
LehtiNature plants
Vuosikerta5
Numero6
Sivut604-615
Sivumäärä12
ISSN2055-026X
DOI - pysyväislinkit
TilaJulkaistu - 10 kesäkuuta 2019
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Lainaa tätä

Yan, Dawei ; Yadav, Shri ; Paterlini, Andrea ; Nicolas, William J ; Petit, Jules D ; Brocard, Lysiane ; Belevich, Ilya ; Grison, Magali S ; Vaten, Anne ; Karami, Leila ; El-Showk, Sedeer ; Lee, Jung-Youn ; Murawska, Gosia M ; Mortimer, Jenny ; Knoblauch, Michael ; Jokitalo, Eija ; Markham, Jonathan E ; Bayer, Emmanuelle M ; Helariutta, Yrjö. / Sphingolipid biosynthesis modulates plasmodesmal ultrastructure and phloem unloading. Julkaisussa: Nature plants. 2019 ; Vuosikerta 5, Nro 6. Sivut 604-615.
@article{a5b4b3258b4941c0936dd4c652aed711,
title = "Sphingolipid biosynthesis modulates plasmodesmal ultrastructure and phloem unloading",
abstract = "During phloem unloading, multiple cell-to-cell transport events move organic substances to the root meristem. Although the primary unloading event from the sieve elements to the phloem pole pericycle has been characterized to some extent, little is known about post-sieve element unloading. Here, we report a novel gene, PHLOEM UNLOADING MODULATOR (PLM), in the absence of which plasmodesmata-mediated symplastic transport through the phloem pole pericycle-endodermis interface is specifically enhanced. Increased unloading is attributable to a defect in the formation of the endoplasmic reticulum-plasma membrane tethers during plasmodesmal morphogenesis, resulting in the majority of pores lacking a visible cytoplasmic sleeve. PLM encodes a putative enzyme required for the biosynthesis of sphingolipids with very-long-chain fatty acid. Taken together, our results indicate that post-sieve element unloading involves sphingolipid metabolism, which affects plasmodesmal ultrastructure. They also raise the question of how and why plasmodesmata with no cytoplasmic sleeve facilitate molecular trafficking.",
author = "Dawei Yan and Shri Yadav and Andrea Paterlini and Nicolas, {William J} and Petit, {Jules D} and Lysiane Brocard and Ilya Belevich and Grison, {Magali S} and Anne Vaten and Leila Karami and Sedeer El-Showk and Jung-Youn Lee and Murawska, {Gosia M} and Jenny Mortimer and Michael Knoblauch and Eija Jokitalo and Markham, {Jonathan E} and Bayer, {Emmanuelle M} and Yrj{\"o} Helariutta",
year = "2019",
month = "6",
day = "10",
doi = "10.1038/s41477-019-0429-5",
language = "English",
volume = "5",
pages = "604--615",
journal = "Nature plants",
issn = "2055-026X",
publisher = "Nature Publishing Group",
number = "6",

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Yan, D, Yadav, S, Paterlini, A, Nicolas, WJ, Petit, JD, Brocard, L, Belevich, I, Grison, MS, Vaten, A, Karami, L, El-Showk, S, Lee, J-Y, Murawska, GM, Mortimer, J, Knoblauch, M, Jokitalo, E, Markham, JE, Bayer, EM & Helariutta, Y 2019, 'Sphingolipid biosynthesis modulates plasmodesmal ultrastructure and phloem unloading' Nature plants, Vuosikerta 5, Nro 6, Sivut 604-615. https://doi.org/10.1038/s41477-019-0429-5

Sphingolipid biosynthesis modulates plasmodesmal ultrastructure and phloem unloading. / Yan, Dawei; Yadav, Shri; Paterlini, Andrea; Nicolas, William J; Petit, Jules D; Brocard, Lysiane; Belevich, Ilya; Grison, Magali S; Vaten, Anne; Karami, Leila; El-Showk, Sedeer; Lee, Jung-Youn; Murawska, Gosia M; Mortimer, Jenny; Knoblauch, Michael; Jokitalo, Eija; Markham, Jonathan E; Bayer, Emmanuelle M; Helariutta, Yrjö.

julkaisussa: Nature plants, Vuosikerta 5, Nro 6, 10.06.2019, s. 604-615.

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - Sphingolipid biosynthesis modulates plasmodesmal ultrastructure and phloem unloading

AU - Yan, Dawei

AU - Yadav, Shri

AU - Paterlini, Andrea

AU - Nicolas, William J

AU - Petit, Jules D

AU - Brocard, Lysiane

AU - Belevich, Ilya

AU - Grison, Magali S

AU - Vaten, Anne

AU - Karami, Leila

AU - El-Showk, Sedeer

AU - Lee, Jung-Youn

AU - Murawska, Gosia M

AU - Mortimer, Jenny

AU - Knoblauch, Michael

AU - Jokitalo, Eija

AU - Markham, Jonathan E

AU - Bayer, Emmanuelle M

AU - Helariutta, Yrjö

PY - 2019/6/10

Y1 - 2019/6/10

N2 - During phloem unloading, multiple cell-to-cell transport events move organic substances to the root meristem. Although the primary unloading event from the sieve elements to the phloem pole pericycle has been characterized to some extent, little is known about post-sieve element unloading. Here, we report a novel gene, PHLOEM UNLOADING MODULATOR (PLM), in the absence of which plasmodesmata-mediated symplastic transport through the phloem pole pericycle-endodermis interface is specifically enhanced. Increased unloading is attributable to a defect in the formation of the endoplasmic reticulum-plasma membrane tethers during plasmodesmal morphogenesis, resulting in the majority of pores lacking a visible cytoplasmic sleeve. PLM encodes a putative enzyme required for the biosynthesis of sphingolipids with very-long-chain fatty acid. Taken together, our results indicate that post-sieve element unloading involves sphingolipid metabolism, which affects plasmodesmal ultrastructure. They also raise the question of how and why plasmodesmata with no cytoplasmic sleeve facilitate molecular trafficking.

AB - During phloem unloading, multiple cell-to-cell transport events move organic substances to the root meristem. Although the primary unloading event from the sieve elements to the phloem pole pericycle has been characterized to some extent, little is known about post-sieve element unloading. Here, we report a novel gene, PHLOEM UNLOADING MODULATOR (PLM), in the absence of which plasmodesmata-mediated symplastic transport through the phloem pole pericycle-endodermis interface is specifically enhanced. Increased unloading is attributable to a defect in the formation of the endoplasmic reticulum-plasma membrane tethers during plasmodesmal morphogenesis, resulting in the majority of pores lacking a visible cytoplasmic sleeve. PLM encodes a putative enzyme required for the biosynthesis of sphingolipids with very-long-chain fatty acid. Taken together, our results indicate that post-sieve element unloading involves sphingolipid metabolism, which affects plasmodesmal ultrastructure. They also raise the question of how and why plasmodesmata with no cytoplasmic sleeve facilitate molecular trafficking.

U2 - 10.1038/s41477-019-0429-5

DO - 10.1038/s41477-019-0429-5

M3 - Article

VL - 5

SP - 604

EP - 615

JO - Nature plants

JF - Nature plants

SN - 2055-026X

IS - 6

ER -

Yan D, Yadav S, Paterlini A, Nicolas WJ, Petit JD, Brocard L et al. Sphingolipid biosynthesis modulates plasmodesmal ultrastructure and phloem unloading. Nature plants. 2019 kesä 10;5(6):604-615. https://doi.org/10.1038/s41477-019-0429-5