Abstrakti
Plant photosynthetic and mitochondrial electron transfer chains (ETCs) are delicate environmental sensors and active players in stress acclimation. The performance of photosynthetic ETC can be deduced from chlorophyll a fluorescence. This makes chlorophyll fluorescence imaging a powerful tool to study plant stress in vivo. Many stress treatments enhance production of reactive oxygen species (ROS) by photosynthetic or mitochondrial ETCs. These ROS affect cellular metabolism and signalling. Generation of ROS can be manipulated in planta by specific pharmacological treatments with methyl viologen (MV), antimycin A (AA), myxothiazol (myx), and salicylhydroxamic acid (SHAM). This chapter describes how chlorophyll fluorescence imaging together with pharmacological treatments can be employed to probe ROS-dependent plant stress reactions in vivo.
| Alkuperäiskieli | englanti |
|---|---|
| Otsikko | Reactive Oxygen Species in Plants : Methods and Protocols |
| Toimittajat | Amna Mhamdi |
| Sivumäärä | 19 |
| Kustantaja | Humana press |
| Julkaisupäivä | 2022 |
| Sivut | 43-61 |
| ISBN (painettu) | 978-1-0716-2468-5, 978-1-0716-2471-5 |
| ISBN (elektroninen) | 978-1-0716-2469-2 |
| DOI - pysyväislinkit | |
| Tila | Julkaistu - 2022 |
| OKM-julkaisutyyppi | A3 Kirjan tai muun kokoomateoksen osa |
Julkaisusarja
| Nimi | Methods in Molecular Biology |
|---|---|
| Vuosikerta | 2526 |
| ISSN (painettu) | 1064-3745 |
| ISSN (elektroninen) | 1940-6029 |
Lisätietoja
Funding Information:We thank Dr. Fuqiang Cui for his assistance in developing the leaf disc assays. We are grateful to Dr. Zuzana Benedikty for optimizing the FluorCam protocol and for revising the manuscript and to Dr. Erhard Pfündel, Dr. Mikael Brosché, and Tuomas Puukko for their helpful comments on the manuscript.
Publisher Copyright:
© 2022, Springer Science+Business Media, LLC, part of Springer Nature.
Tieteenalat
- 11831 Kasvibiologia