Sulphite addition during steam pretreatment enhanced both enzyme-mediated cellulose hydrolysis and ethanol production

Na Zhong, Richard Chandra, Minna Yamamoto, Timo Leskinen, Tom Granström, Jack Saddler

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Abstrakti

Sulphite addition during steam pretreatment of softwoods under acidic, neutral and alkaline conditions was assessed to try to minimize lignin condensation. Although pretreatment under neutral/alkaline conditions resulted in effective lignin sulphonation, non-uniform size reduction was observed. In contrast, acidic sulphite steam treatment at 210 °C for 10 min resulted in homogenous particle size reduction and water-insoluble component that was 62% carbohydrate and 33% lignin. This carbohydrate-rich substrate was readily hydrolyzed and fermented which indicated the lack of fermentation inhibitors in the steam-pretreated whole slurry. The use of high solid loading (25% w/v) resulted in a hydrolysis yield of 58% at an enzyme loading of 40 mg protein/g glucan and efficient fermentation (46.6 g/L of ethanol). This indicated that the addition of acidic sulphite at the steam pretreatment of softwoods improved both the enzymatic hydrolysis and fermentation of steam-pretreated whole slurries. Graphical Abstract: [Figure not available: see fulltext.]

Alkuperäiskielienglanti
Artikkeli71
LehtiBioresources and bioprocessing
Vuosikerta9
Numero1
Sivumäärä9
ISSN2197-4365
DOI - pysyväislinkit
TilaJulkaistu - jouluk. 2022
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Lisätietoja

Funding Information:
The authors wish to thank China Scholarship Council and Mitacs for funding this research.

Funding Information:
We acknowledge Novozymes for their generous gift of cellulase enzymes mixtures.

Publisher Copyright:
© 2022, The Author(s).

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