Abstrakti
Sulphite addition during steam pretreatment of softwoods under acidic, neutral and alkaline conditions was assessed to try to minimize lignin condensation. Although pretreatment under neutral/alkaline conditions resulted in effective lignin sulphonation, non-uniform size reduction was observed. In contrast, acidic sulphite steam treatment at 210 °C for 10 min resulted in homogenous particle size reduction and water-insoluble component that was 62% carbohydrate and 33% lignin. This carbohydrate-rich substrate was readily hydrolyzed and fermented which indicated the lack of fermentation inhibitors in the steam-pretreated whole slurry. The use of high solid loading (25% w/v) resulted in a hydrolysis yield of 58% at an enzyme loading of 40 mg protein/g glucan and efficient fermentation (46.6 g/L of ethanol). This indicated that the addition of acidic sulphite at the steam pretreatment of softwoods improved both the enzymatic hydrolysis and fermentation of steam-pretreated whole slurries. Graphical Abstract: [Figure not available: see fulltext.]
Alkuperäiskieli | englanti |
---|---|
Artikkeli | 71 |
Lehti | Bioresources and bioprocessing |
Vuosikerta | 9 |
Numero | 1 |
Sivumäärä | 9 |
ISSN | 2197-4365 |
DOI - pysyväislinkit | |
Tila | Julkaistu - jouluk. 2022 |
OKM-julkaisutyyppi | A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu |
Lisätietoja
Funding Information:The authors wish to thank China Scholarship Council and Mitacs for funding this research.
Funding Information:
We acknowledge Novozymes for their generous gift of cellulase enzymes mixtures.
Publisher Copyright:
© 2022, The Author(s).
Tieteenalat
- 116 Kemia