Temporal variation in indoor transfer of dirt-associated environmental bacteria in agricultural and urban areas

Nan Hui, Anirudra Parajuli, Riikka Puhakka, Mira Grönroos, Marja Roslund, Heli Vari, Ville A. O. Selonen, Guoyang Yan, Nathan Siter, Noora Nurminen, Sami Oikarinen, Olli Laitinen, Juho Rajaniemi, Heikki Hyöty, Aki Sinkkonen

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

An agricultural environment and exposure to diverse environmental microbiota has been suggested to confer protection against immune-mediated disorders. As an agricultural environment may have a protective role, it is crucial to determine whether the limiting factors in the transfer of environmental microbiota indoors are the same in the agricultural and urban environments. We explored how sampling month, garden diversity and animal ownership affected the indoor-transfer of environmental microbial community. We collected litter from standardized doormats used for 2 weeks in June and August 2015 and February 2016 and identified bacterial phylotypes using 16S rRNA Illumina MiSeq sequencing. In February, the diversity and richness of the whole bacterial community and the relative abundance of environment-associated taxa were reduced, whereas human-associated taxa and genera containing opportunistic pathogens were enriched in the doormats. In summer, the relative abundances of several taxa associated previously with beneficial health effects were higher, particularly in agricultural areas. Surprisingly, the importance of vegetation on doormat microbiota was more observable in February, which may have resulted from snow cover that prevented contact with microbes in soil. Animal ownership increased the prevalence of genera Bacteroides and Acinetobacter in rural doormats. These findings underline the roles of season, living environment and lifestyle in the temporal variations in the environmental microbial community carried indoors. As reduced contact with diverse microbiota is a potential reason for immune system dysfunction, the results may have important implications in the etiology of immune-mediated, non-communicable diseases.
Alkuperäiskielienglanti
Artikkeli105069
LehtiEnvironment International
Vuosikerta132
Sivumäärä9
ISSN0160-4120
DOI - pysyväislinkit
TilaJulkaistu - marraskuuta 2019
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 1172 Ympäristötiede

Lainaa tätä

Hui, Nan ; Parajuli, Anirudra ; Puhakka, Riikka ; Grönroos, Mira ; Roslund, Marja ; Vari, Heli ; Selonen, Ville A. O. ; Yan, Guoyang ; Siter, Nathan ; Nurminen, Noora ; Oikarinen, Sami ; Laitinen, Olli ; Rajaniemi, Juho ; Hyöty, Heikki ; Sinkkonen, Aki. / Temporal variation in indoor transfer of dirt-associated environmental bacteria in agricultural and urban areas. Julkaisussa: Environment International. 2019 ; Vuosikerta 132.
@article{3a2498724e9f4a7ba01c7e02d1d45d71,
title = "Temporal variation in indoor transfer of dirt-associated environmental bacteria in agricultural and urban areas",
abstract = "An agricultural environment and exposure to diverse environmental microbiota has been suggested to confer protection against immune-mediated disorders. As an agricultural environment may have a protective role, it is crucial to determine whether the limiting factors in the transfer of environmental microbiota indoors are the same in the agricultural and urban environments. We explored how sampling month, garden diversity and animal ownership affected the indoor-transfer of environmental microbial community. We collected litter from standardized doormats used for 2 weeks in June and August 2015 and February 2016 and identified bacterial phylotypes using 16S rRNA Illumina MiSeq sequencing. In February, the diversity and richness of the whole bacterial community and the relative abundance of environment-associated taxa were reduced, whereas human-associated taxa and genera containing opportunistic pathogens were enriched in the doormats. In summer, the relative abundances of several taxa associated previously with beneficial health effects were higher, particularly in agricultural areas. Surprisingly, the importance of vegetation on doormat microbiota was more observable in February, which may have resulted from snow cover that prevented contact with microbes in soil. Animal ownership increased the prevalence of genera Bacteroides and Acinetobacter in rural doormats. These findings underline the roles of season, living environment and lifestyle in the temporal variations in the environmental microbial community carried indoors. As reduced contact with diverse microbiota is a potential reason for immune system dysfunction, the results may have important implications in the etiology of immune-mediated, non-communicable diseases.",
keywords = "1172 Environmental sciences, Environmental microbiota, Indoor microbiota, Temporal variation, Vegetation, MICROBIAL COMMUNITIES, AIR, DISEASE, ALLERGY, BIODIVERSITY, SEASONALITY, SEQUENCES, EXPOSURE, HYGIENE, HEALTH",
author = "Nan Hui and Anirudra Parajuli and Riikka Puhakka and Mira Gr{\"o}nroos and Marja Roslund and Heli Vari and Selonen, {Ville A. O.} and Guoyang Yan and Nathan Siter and Noora Nurminen and Sami Oikarinen and Olli Laitinen and Juho Rajaniemi and Heikki Hy{\"o}ty and Aki Sinkkonen",
year = "2019",
month = "11",
doi = "10.1016/j.envint.2019.105069",
language = "English",
volume = "132",
journal = "Environment International",
issn = "0160-4120",
publisher = "Elsevier Scientific Publ. Co",

}

Temporal variation in indoor transfer of dirt-associated environmental bacteria in agricultural and urban areas. / Hui, Nan; Parajuli, Anirudra; Puhakka, Riikka; Grönroos, Mira; Roslund, Marja; Vari, Heli; Selonen, Ville A. O.; Yan, Guoyang; Siter, Nathan; Nurminen, Noora; Oikarinen, Sami; Laitinen, Olli; Rajaniemi, Juho; Hyöty, Heikki; Sinkkonen, Aki.

julkaisussa: Environment International, Vuosikerta 132, 105069, 11.2019.

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - Temporal variation in indoor transfer of dirt-associated environmental bacteria in agricultural and urban areas

AU - Hui, Nan

AU - Parajuli, Anirudra

AU - Puhakka, Riikka

AU - Grönroos, Mira

AU - Roslund, Marja

AU - Vari, Heli

AU - Selonen, Ville A. O.

AU - Yan, Guoyang

AU - Siter, Nathan

AU - Nurminen, Noora

AU - Oikarinen, Sami

AU - Laitinen, Olli

AU - Rajaniemi, Juho

AU - Hyöty, Heikki

AU - Sinkkonen, Aki

PY - 2019/11

Y1 - 2019/11

N2 - An agricultural environment and exposure to diverse environmental microbiota has been suggested to confer protection against immune-mediated disorders. As an agricultural environment may have a protective role, it is crucial to determine whether the limiting factors in the transfer of environmental microbiota indoors are the same in the agricultural and urban environments. We explored how sampling month, garden diversity and animal ownership affected the indoor-transfer of environmental microbial community. We collected litter from standardized doormats used for 2 weeks in June and August 2015 and February 2016 and identified bacterial phylotypes using 16S rRNA Illumina MiSeq sequencing. In February, the diversity and richness of the whole bacterial community and the relative abundance of environment-associated taxa were reduced, whereas human-associated taxa and genera containing opportunistic pathogens were enriched in the doormats. In summer, the relative abundances of several taxa associated previously with beneficial health effects were higher, particularly in agricultural areas. Surprisingly, the importance of vegetation on doormat microbiota was more observable in February, which may have resulted from snow cover that prevented contact with microbes in soil. Animal ownership increased the prevalence of genera Bacteroides and Acinetobacter in rural doormats. These findings underline the roles of season, living environment and lifestyle in the temporal variations in the environmental microbial community carried indoors. As reduced contact with diverse microbiota is a potential reason for immune system dysfunction, the results may have important implications in the etiology of immune-mediated, non-communicable diseases.

AB - An agricultural environment and exposure to diverse environmental microbiota has been suggested to confer protection against immune-mediated disorders. As an agricultural environment may have a protective role, it is crucial to determine whether the limiting factors in the transfer of environmental microbiota indoors are the same in the agricultural and urban environments. We explored how sampling month, garden diversity and animal ownership affected the indoor-transfer of environmental microbial community. We collected litter from standardized doormats used for 2 weeks in June and August 2015 and February 2016 and identified bacterial phylotypes using 16S rRNA Illumina MiSeq sequencing. In February, the diversity and richness of the whole bacterial community and the relative abundance of environment-associated taxa were reduced, whereas human-associated taxa and genera containing opportunistic pathogens were enriched in the doormats. In summer, the relative abundances of several taxa associated previously with beneficial health effects were higher, particularly in agricultural areas. Surprisingly, the importance of vegetation on doormat microbiota was more observable in February, which may have resulted from snow cover that prevented contact with microbes in soil. Animal ownership increased the prevalence of genera Bacteroides and Acinetobacter in rural doormats. These findings underline the roles of season, living environment and lifestyle in the temporal variations in the environmental microbial community carried indoors. As reduced contact with diverse microbiota is a potential reason for immune system dysfunction, the results may have important implications in the etiology of immune-mediated, non-communicable diseases.

KW - 1172 Environmental sciences

KW - Environmental microbiota

KW - Indoor microbiota

KW - Temporal variation

KW - Vegetation

KW - MICROBIAL COMMUNITIES

KW - AIR

KW - DISEASE

KW - ALLERGY

KW - BIODIVERSITY

KW - SEASONALITY

KW - SEQUENCES

KW - EXPOSURE

KW - HYGIENE

KW - HEALTH

U2 - 10.1016/j.envint.2019.105069

DO - 10.1016/j.envint.2019.105069

M3 - Article

VL - 132

JO - Environment International

JF - Environment International

SN - 0160-4120

M1 - 105069

ER -