The Y-Box Binding Protein 1 Suppresses Alzheimer’s Disease Progression in Two Animal Models

Natalia Bobkova, Dmitry Lyabin, Natalia Medvinskaya, Alexei Samokhin, Pavel Nekrasov, Irina Nesterova, I. Y. Aleksandrova, O. G. Tatarnikova, A. G. Bobylev, I. M. Vikhlyantsev, M. S. Kukharsky, A. A. Ustyugov, Dmitry Polyakov, Irina Eliseeva, Dmitry Kretov, Sergey Guryanov, Lev Ovchinnikov

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

The Y-box binding protein 1 (YB-1) is a member of the family of DNA- and RNA binding proteins. It is involved in a wide variety of DNA/RNA-dependent events including cell proliferation and differentiation, stress response, and malignant cell transformation. Previously, YB-1 was detected in neurons of the neocortex and hippocampus, but its precise role in the brain remains undefined. Here we show that subchronic intranasal injections of recombinant YB-1, as well as its fragment YB-11−219, suppress impairment of spatial memory in olfactory bulbectomized (OBX) mice with Alzheimer’s type degeneration and improve learning in transgenic 5XFAD mice used as a model of cerebral amyloidosis. YB-1-treated OBX and 5XFAD mice showed a decreased level of brain β-amyloid. In OBX animals, an improved morphological state of neurons was revealed in the neocortex and hippocampus; in 5XFAD mice, a delay in amyloid plaque progression was observed. Intranasally administered YB-1 penetrated into the brain and could enter neurons. In vitro co-incubation of YB-1 with monomeric β-amyloid (1–42) inhibited formation of β-amyloid fibrils, as confirmed by electron microscopy. This suggests that YB-1 interaction with β-amyloid prevents formation of filaments that are responsible for neurotoxicity and neuronal death. Our data are the first evidence for a potential therapeutic benefit of YB-1 for treatment of Alzheimer’s disease.
Alkuperäiskielienglanti
Artikkelie0138867
LehtiPLoS One
Vuosikerta10
Numero9
ISSN1932-6203
TilaJulkaistu - 22 syyskuuta 2015
Julkaistu ulkoisestiKyllä
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 1182 Biokemia, solu- ja molekyylibiologia
  • 1184 Genetiikka, kehitysbiologia, fysiologia

Lainaa tätä

Bobkova, N., Lyabin, D., Medvinskaya, N., Samokhin, A., Nekrasov, P., Nesterova, I., ... Ovchinnikov, L. (2015). The Y-Box Binding Protein 1 Suppresses Alzheimer’s Disease Progression in Two Animal Models. PLoS One, 10(9), [e0138867].
Bobkova, Natalia ; Lyabin, Dmitry ; Medvinskaya, Natalia ; Samokhin, Alexei ; Nekrasov, Pavel ; Nesterova, Irina ; Aleksandrova, I. Y. ; Tatarnikova, O. G. ; Bobylev, A. G. ; Vikhlyantsev, I. M. ; Kukharsky, M. S. ; Ustyugov, A. A. ; Polyakov, Dmitry ; Eliseeva, Irina ; Kretov, Dmitry ; Guryanov, Sergey ; Ovchinnikov, Lev. / The Y-Box Binding Protein 1 Suppresses Alzheimer’s Disease Progression in Two Animal Models. Julkaisussa: PLoS One. 2015 ; Vuosikerta 10, Nro 9.
@article{c0c01f285eca4c27bbd253bb772737db,
title = "The Y-Box Binding Protein 1 Suppresses Alzheimer’s Disease Progression in Two Animal Models",
abstract = "The Y-box binding protein 1 (YB-1) is a member of the family of DNA- and RNA binding proteins. It is involved in a wide variety of DNA/RNA-dependent events including cell proliferation and differentiation, stress response, and malignant cell transformation. Previously, YB-1 was detected in neurons of the neocortex and hippocampus, but its precise role in the brain remains undefined. Here we show that subchronic intranasal injections of recombinant YB-1, as well as its fragment YB-11−219, suppress impairment of spatial memory in olfactory bulbectomized (OBX) mice with Alzheimer’s type degeneration and improve learning in transgenic 5XFAD mice used as a model of cerebral amyloidosis. YB-1-treated OBX and 5XFAD mice showed a decreased level of brain β-amyloid. In OBX animals, an improved morphological state of neurons was revealed in the neocortex and hippocampus; in 5XFAD mice, a delay in amyloid plaque progression was observed. Intranasally administered YB-1 penetrated into the brain and could enter neurons. In vitro co-incubation of YB-1 with monomeric β-amyloid (1–42) inhibited formation of β-amyloid fibrils, as confirmed by electron microscopy. This suggests that YB-1 interaction with β-amyloid prevents formation of filaments that are responsible for neurotoxicity and neuronal death. Our data are the first evidence for a potential therapeutic benefit of YB-1 for treatment of Alzheimer’s disease.",
keywords = "1182 Biochemistry, cell and molecular biology, AMYLOID BETA-PEPTIDE, Cold shock domain, 1184 Genetics, developmental biology, physiology",
author = "Natalia Bobkova and Dmitry Lyabin and Natalia Medvinskaya and Alexei Samokhin and Pavel Nekrasov and Irina Nesterova and Aleksandrova, {I. Y.} and Tatarnikova, {O. G.} and Bobylev, {A. G.} and Vikhlyantsev, {I. M.} and Kukharsky, {M. S.} and Ustyugov, {A. A.} and Dmitry Polyakov and Irina Eliseeva and Dmitry Kretov and Sergey Guryanov and Lev Ovchinnikov",
year = "2015",
month = "9",
day = "22",
language = "English",
volume = "10",
journal = "PLoS One",
issn = "1932-6203",
publisher = "PUBLIC LIBRARY OF SCIENCE",
number = "9",

}

Bobkova, N, Lyabin, D, Medvinskaya, N, Samokhin, A, Nekrasov, P, Nesterova, I, Aleksandrova, IY, Tatarnikova, OG, Bobylev, AG, Vikhlyantsev, IM, Kukharsky, MS, Ustyugov, AA, Polyakov, D, Eliseeva, I, Kretov, D, Guryanov, S & Ovchinnikov, L 2015, 'The Y-Box Binding Protein 1 Suppresses Alzheimer’s Disease Progression in Two Animal Models', PLoS One, Vuosikerta 10, Nro 9, e0138867.

The Y-Box Binding Protein 1 Suppresses Alzheimer’s Disease Progression in Two Animal Models. / Bobkova, Natalia; Lyabin, Dmitry; Medvinskaya, Natalia; Samokhin, Alexei; Nekrasov, Pavel; Nesterova, Irina; Aleksandrova, I. Y.; Tatarnikova, O. G.; Bobylev, A. G.; Vikhlyantsev, I. M.; Kukharsky, M. S.; Ustyugov, A. A.; Polyakov, Dmitry; Eliseeva, Irina; Kretov, Dmitry; Guryanov, Sergey; Ovchinnikov, Lev.

julkaisussa: PLoS One, Vuosikerta 10, Nro 9, e0138867, 22.09.2015.

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - The Y-Box Binding Protein 1 Suppresses Alzheimer’s Disease Progression in Two Animal Models

AU - Bobkova, Natalia

AU - Lyabin, Dmitry

AU - Medvinskaya, Natalia

AU - Samokhin, Alexei

AU - Nekrasov, Pavel

AU - Nesterova, Irina

AU - Aleksandrova, I. Y.

AU - Tatarnikova, O. G.

AU - Bobylev, A. G.

AU - Vikhlyantsev, I. M.

AU - Kukharsky, M. S.

AU - Ustyugov, A. A.

AU - Polyakov, Dmitry

AU - Eliseeva, Irina

AU - Kretov, Dmitry

AU - Guryanov, Sergey

AU - Ovchinnikov, Lev

PY - 2015/9/22

Y1 - 2015/9/22

N2 - The Y-box binding protein 1 (YB-1) is a member of the family of DNA- and RNA binding proteins. It is involved in a wide variety of DNA/RNA-dependent events including cell proliferation and differentiation, stress response, and malignant cell transformation. Previously, YB-1 was detected in neurons of the neocortex and hippocampus, but its precise role in the brain remains undefined. Here we show that subchronic intranasal injections of recombinant YB-1, as well as its fragment YB-11−219, suppress impairment of spatial memory in olfactory bulbectomized (OBX) mice with Alzheimer’s type degeneration and improve learning in transgenic 5XFAD mice used as a model of cerebral amyloidosis. YB-1-treated OBX and 5XFAD mice showed a decreased level of brain β-amyloid. In OBX animals, an improved morphological state of neurons was revealed in the neocortex and hippocampus; in 5XFAD mice, a delay in amyloid plaque progression was observed. Intranasally administered YB-1 penetrated into the brain and could enter neurons. In vitro co-incubation of YB-1 with monomeric β-amyloid (1–42) inhibited formation of β-amyloid fibrils, as confirmed by electron microscopy. This suggests that YB-1 interaction with β-amyloid prevents formation of filaments that are responsible for neurotoxicity and neuronal death. Our data are the first evidence for a potential therapeutic benefit of YB-1 for treatment of Alzheimer’s disease.

AB - The Y-box binding protein 1 (YB-1) is a member of the family of DNA- and RNA binding proteins. It is involved in a wide variety of DNA/RNA-dependent events including cell proliferation and differentiation, stress response, and malignant cell transformation. Previously, YB-1 was detected in neurons of the neocortex and hippocampus, but its precise role in the brain remains undefined. Here we show that subchronic intranasal injections of recombinant YB-1, as well as its fragment YB-11−219, suppress impairment of spatial memory in olfactory bulbectomized (OBX) mice with Alzheimer’s type degeneration and improve learning in transgenic 5XFAD mice used as a model of cerebral amyloidosis. YB-1-treated OBX and 5XFAD mice showed a decreased level of brain β-amyloid. In OBX animals, an improved morphological state of neurons was revealed in the neocortex and hippocampus; in 5XFAD mice, a delay in amyloid plaque progression was observed. Intranasally administered YB-1 penetrated into the brain and could enter neurons. In vitro co-incubation of YB-1 with monomeric β-amyloid (1–42) inhibited formation of β-amyloid fibrils, as confirmed by electron microscopy. This suggests that YB-1 interaction with β-amyloid prevents formation of filaments that are responsible for neurotoxicity and neuronal death. Our data are the first evidence for a potential therapeutic benefit of YB-1 for treatment of Alzheimer’s disease.

KW - 1182 Biochemistry, cell and molecular biology

KW - AMYLOID BETA-PEPTIDE

KW - Cold shock domain

KW - 1184 Genetics, developmental biology, physiology

UR - http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0138867

M3 - Article

VL - 10

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 9

M1 - e0138867

ER -

Bobkova N, Lyabin D, Medvinskaya N, Samokhin A, Nekrasov P, Nesterova I et al. The Y-Box Binding Protein 1 Suppresses Alzheimer’s Disease Progression in Two Animal Models. PLoS One. 2015 syys 22;10(9). e0138867.