Three water restriction schedules used in rodent behavioral tasks transiently impair growth and differentially evoke a stress hormone response without causing dehydration

Dmitrii Vasilev, Daniel Havel, Simone Liebscher, Silvia Slesiona-Kuenzel, Nikos K. Logothetis, Katja Schenke-Layland, Nelson Totah

Tutkimustuotos: ArtikkelijulkaisuArtikkelivertaisarvioitu

Abstrakti

Water restriction is commonly used to motivate rodents to perform behavioral tasks; however, its effects on hydration and stress hormone levels are unknown. Here, we report daily body weight and bi-weekly packed red blood cell volume and corticosterone in adult male rats across 80 days for three commonly used water restriction schedules. We also assessed renal adaptation to water restriction using post-mortem histological evaluation of renal medulla. A control group received ad libitum water. After one week of water restriction, rats on all restriction schedules resumed similar levels of growth relative to the control group. Normal hydration was observed, and water restriction did not drive renal adaptation. An intermittent restriction schedule was associated with an increase in corticosterone relative to the control group. However, intermittent restriction evokes a stress response which could affect behavioral and neurobiological results. Our results also suggest that stable motivation in behavioral tasks may only be achieved after one week of restriction.
Alkuperäiskielienglanti
ArtikkeliARTN 0424-21.2021
LehtieNeuro
Vuosikerta8
Numero6
Sivumäärä11
ISSN2373-2822
DOI - pysyväislinkit
TilaJulkaistu - marraskuuta 2021
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 3112 Neurotieteet

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