Transcriptional analysis of petal organogenesis in Gerbera hybrida

Roosa Laitinen, Eija Pöllänen, Teemu Teeri, Paula Elomaa, Mika Kotilainen

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

Kuvaus

Understanding of the molecular interplay, which determines early steps of flower formation has grown considerably during last years. In contrast, genetic actions responsible for how flower organs acquire their size and shape at later phases of organogenesis are still poorly understood. We have exploited the large and anatomically simple Gerbera (Gerbera hybrida var. Terra regina) ray flower petals to describe transcriptional changes during organogenesis. Gerbera 9 K cDNA microarray was utilized to profile gene expression at six different developmental stages of petal organogenesis, at the earliest stage expansion of petals is starting and at the latest stage petals have reached their final size and shape. Genes potentially participating in petal opening were identified based on the similarity in expression with a known marker gene. Our results showed characteristic sets of genes expressed during the cell division and cell expansion phases of petal development. Interestingly, there was a transition stage during which neither cell division nor cell expansion marker genes were abundantly expressed. Moreover, constitutive expression of late petal specific genes indicates that they participate in petal organogenesis throughout the development and they are not involved in stage specific switch points.
Alkuperäiskielienglanti
LehtiPlanta
Vuosikerta226
Sivut347-360
Sivumäärä14
ISSN0032-0935
DOI - pysyväislinkit
TilaJulkaistu - 2007
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Tieteenalat

  • 414 Maatalouden bioteknologia
  • 411 Maatalous ja metsätieteet

Lainaa tätä

Laitinen, Roosa ; Pöllänen, Eija ; Teeri, Teemu ; Elomaa, Paula ; Kotilainen, Mika. / Transcriptional analysis of petal organogenesis in Gerbera hybrida. Julkaisussa: Planta. 2007 ; Vuosikerta 226. Sivut 347-360.
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title = "Transcriptional analysis of petal organogenesis in Gerbera hybrida",
abstract = "Understanding of the molecular interplay, which determines early steps of flower formation has grown considerably during last years. In contrast, genetic actions responsible for how flower organs acquire their size and shape at later phases of organogenesis are still poorly understood. We have exploited the large and anatomically simple Gerbera (Gerbera hybrida var. Terra regina) ray flower petals to describe transcriptional changes during organogenesis. Gerbera 9 K cDNA microarray was utilized to profile gene expression at six different developmental stages of petal organogenesis, at the earliest stage expansion of petals is starting and at the latest stage petals have reached their final size and shape. Genes potentially participating in petal opening were identified based on the similarity in expression with a known marker gene. Our results showed characteristic sets of genes expressed during the cell division and cell expansion phases of petal development. Interestingly, there was a transition stage during which neither cell division nor cell expansion marker genes were abundantly expressed. Moreover, constitutive expression of late petal specific genes indicates that they participate in petal organogenesis throughout the development and they are not involved in stage specific switch points.",
keywords = "414 Agricultural biotechnology, 411 Agriculture and forestry",
author = "Roosa Laitinen and Eija P{\"o}ll{\"a}nen and Teemu Teeri and Paula Elomaa and Mika Kotilainen",
year = "2007",
doi = "10.1007/s00425-007-0486-2",
language = "English",
volume = "226",
pages = "347--360",
journal = "Planta",
issn = "0032-0935",
publisher = "Springer",

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Transcriptional analysis of petal organogenesis in Gerbera hybrida. / Laitinen, Roosa; Pöllänen, Eija; Teeri, Teemu; Elomaa, Paula; Kotilainen, Mika.

julkaisussa: Planta, Vuosikerta 226, 2007, s. 347-360.

Tutkimustuotos: ArtikkelijulkaisuArtikkeliTieteellinenvertaisarvioitu

TY - JOUR

T1 - Transcriptional analysis of petal organogenesis in Gerbera hybrida

AU - Laitinen, Roosa

AU - Pöllänen, Eija

AU - Teeri, Teemu

AU - Elomaa, Paula

AU - Kotilainen, Mika

PY - 2007

Y1 - 2007

N2 - Understanding of the molecular interplay, which determines early steps of flower formation has grown considerably during last years. In contrast, genetic actions responsible for how flower organs acquire their size and shape at later phases of organogenesis are still poorly understood. We have exploited the large and anatomically simple Gerbera (Gerbera hybrida var. Terra regina) ray flower petals to describe transcriptional changes during organogenesis. Gerbera 9 K cDNA microarray was utilized to profile gene expression at six different developmental stages of petal organogenesis, at the earliest stage expansion of petals is starting and at the latest stage petals have reached their final size and shape. Genes potentially participating in petal opening were identified based on the similarity in expression with a known marker gene. Our results showed characteristic sets of genes expressed during the cell division and cell expansion phases of petal development. Interestingly, there was a transition stage during which neither cell division nor cell expansion marker genes were abundantly expressed. Moreover, constitutive expression of late petal specific genes indicates that they participate in petal organogenesis throughout the development and they are not involved in stage specific switch points.

AB - Understanding of the molecular interplay, which determines early steps of flower formation has grown considerably during last years. In contrast, genetic actions responsible for how flower organs acquire their size and shape at later phases of organogenesis are still poorly understood. We have exploited the large and anatomically simple Gerbera (Gerbera hybrida var. Terra regina) ray flower petals to describe transcriptional changes during organogenesis. Gerbera 9 K cDNA microarray was utilized to profile gene expression at six different developmental stages of petal organogenesis, at the earliest stage expansion of petals is starting and at the latest stage petals have reached their final size and shape. Genes potentially participating in petal opening were identified based on the similarity in expression with a known marker gene. Our results showed characteristic sets of genes expressed during the cell division and cell expansion phases of petal development. Interestingly, there was a transition stage during which neither cell division nor cell expansion marker genes were abundantly expressed. Moreover, constitutive expression of late petal specific genes indicates that they participate in petal organogenesis throughout the development and they are not involved in stage specific switch points.

KW - 414 Agricultural biotechnology

KW - 411 Agriculture and forestry

U2 - 10.1007/s00425-007-0486-2

DO - 10.1007/s00425-007-0486-2

M3 - Article

VL - 226

SP - 347

EP - 360

JO - Planta

JF - Planta

SN - 0032-0935

ER -