VEGF-C adenovirus gene transfer reduces intima formation in rabbits

Michael Jeltsch, Mikko O Hiltunen, Marja Laitinen, Mikko P Turunen, Juha Hartikainen, Tuomas T Rissanen, Johanna Laukkanen, Mari Niemi, Maija Kossila, Tomi P Häkkinen, Antti Kivelä, Berndt Christian Enholm, Hannu Mansukoski, Anna-Mari Turunen, Kari Alitalo, Seppo Ylä-Herttala

Tutkimustuotos: Kirja/raporttiKokoomateos tai erikoisnumeroTieteellinenvertaisarvioitu

Kuvaus

Background: Gene transfer may provide new possibilities for the treat- ment of postangioplasty restenosis. In this study we analyzed the effects of adenovirus-mediated VEGF-C gene transfer on neointima formation after endothelial denudation in rabbits. For comparison, a second group was treated with VEGF-A adenovirus and a third group with lacZ adenovirus. Methods and Results: Aortas of cholesterol-fed New Zealand White rab- bits were balloon denuded and gene transfer was performed three days later. Animals were sacrificed 2 and 4 weeks after the gene transfer and intima/media ratio (I/M), histology and cell proliferation were analyzed. Two weeks after the gene transfer I/M in the lacZ-transfected control group was 0.57 4- 0.04. VEGF-C gene transfer reduced I/M to 0.38 4- 0.02 (P < 0.05 vs. /acZ group). I/M in VEGF-A treated animals was 0.49 =t=0.17 (ns). Expression of VEGF receptors 1, 2 and 3 were detected in the vessel wall by using immunocytochemistry and in situ hybridization. Conclusions: VEGF-C adenovirus gene transfer is effective in reducing in- timal thickening. VEGF-C may be useful for the treatment of postangioplasty restenosis and vessel wall thickening after vascular manipulations.
Alkuperäiskielienglanti
Vuosikerta151
Painos1
Sivumäärä1
DOI - pysyväislinkit
TilaJulkaistu - heinäkuuta 2000
OKM-julkaisutyyppiC2 Toimitettu teos

Julkaisusarja

NimiAtherosclerosis
Numero1
Vuosikerta151

Lainaa tätä

Jeltsch, M., Hiltunen, M. O., Laitinen, M., Turunen, M. P., Hartikainen, J., Rissanen, T. T., ... Ylä-Herttala, S. (2000). VEGF-C adenovirus gene transfer reduces intima formation in rabbits. (1 toim.) (Atherosclerosis; Vuosikerta 151, Nro 1). https://doi.org/10.1016/S0021-9150(00)80366-4
Jeltsch, Michael ; Hiltunen, Mikko O ; Laitinen, Marja ; Turunen, Mikko P ; Hartikainen, Juha ; Rissanen, Tuomas T ; Laukkanen, Johanna ; Niemi, Mari ; Kossila, Maija ; Häkkinen, Tomi P ; Kivelä, Antti ; Enholm, Berndt Christian ; Mansukoski, Hannu ; Turunen, Anna-Mari ; Alitalo, Kari ; Ylä-Herttala, Seppo. / VEGF-C adenovirus gene transfer reduces intima formation in rabbits. 1 toim. 2000. 1 Sivumäärä (Atherosclerosis; 1).
@book{a1f6a42b17c34732bc7de32786856178,
title = "VEGF-C adenovirus gene transfer reduces intima formation in rabbits",
abstract = "Background: Gene transfer may provide new possibilities for the treat- ment of postangioplasty restenosis. In this study we analyzed the effects of adenovirus-mediated VEGF-C gene transfer on neointima formation after endothelial denudation in rabbits. For comparison, a second group was treated with VEGF-A adenovirus and a third group with lacZ adenovirus. Methods and Results: Aortas of cholesterol-fed New Zealand White rab- bits were balloon denuded and gene transfer was performed three days later. Animals were sacrificed 2 and 4 weeks after the gene transfer and intima/media ratio (I/M), histology and cell proliferation were analyzed. Two weeks after the gene transfer I/M in the lacZ-transfected control group was 0.57 4- 0.04. VEGF-C gene transfer reduced I/M to 0.38 4- 0.02 (P < 0.05 vs. /acZ group). I/M in VEGF-A treated animals was 0.49 =t=0.17 (ns). Expression of VEGF receptors 1, 2 and 3 were detected in the vessel wall by using immunocytochemistry and in situ hybridization. Conclusions: VEGF-C adenovirus gene transfer is effective in reducing in- timal thickening. VEGF-C may be useful for the treatment of postangioplasty restenosis and vessel wall thickening after vascular manipulations.",
author = "Michael Jeltsch and Hiltunen, {Mikko O} and Marja Laitinen and Turunen, {Mikko P} and Juha Hartikainen and Rissanen, {Tuomas T} and Johanna Laukkanen and Mari Niemi and Maija Kossila and H{\"a}kkinen, {Tomi P} and Antti Kivel{\"a} and Enholm, {Berndt Christian} and Hannu Mansukoski and Anna-Mari Turunen and Kari Alitalo and Seppo Yl{\"a}-Herttala",
year = "2000",
month = "7",
doi = "10.1016/S0021-9150(00)80366-4",
language = "English",
volume = "151",
series = "Atherosclerosis",
number = "1",
edition = "1",

}

Jeltsch, M, Hiltunen, MO, Laitinen, M, Turunen, MP, Hartikainen, J, Rissanen, TT, Laukkanen, J, Niemi, M, Kossila, M, Häkkinen, TP, Kivelä, A, Enholm, BC, Mansukoski, H, Turunen, A-M, Alitalo, K & Ylä-Herttala, S 2000, VEGF-C adenovirus gene transfer reduces intima formation in rabbits. Atherosclerosis, Nro 1, Vuosikerta 151, Vuosikerta 151, 1 toim. https://doi.org/10.1016/S0021-9150(00)80366-4

VEGF-C adenovirus gene transfer reduces intima formation in rabbits. / Jeltsch, Michael; Hiltunen, Mikko O; Laitinen, Marja; Turunen, Mikko P; Hartikainen, Juha; Rissanen, Tuomas T; Laukkanen, Johanna; Niemi, Mari; Kossila, Maija; Häkkinen, Tomi P; Kivelä, Antti; Enholm, Berndt Christian; Mansukoski, Hannu; Turunen, Anna-Mari; Alitalo, Kari; Ylä-Herttala, Seppo.

1 toim. 2000. 1 s. (Atherosclerosis; Vuosikerta 151, Nro 1).

Tutkimustuotos: Kirja/raporttiKokoomateos tai erikoisnumeroTieteellinenvertaisarvioitu

TY - BOOK

T1 - VEGF-C adenovirus gene transfer reduces intima formation in rabbits

AU - Jeltsch, Michael

AU - Hiltunen, Mikko O

AU - Laitinen, Marja

AU - Turunen, Mikko P

AU - Hartikainen, Juha

AU - Rissanen, Tuomas T

AU - Laukkanen, Johanna

AU - Niemi, Mari

AU - Kossila, Maija

AU - Häkkinen, Tomi P

AU - Kivelä, Antti

AU - Enholm, Berndt Christian

AU - Mansukoski, Hannu

AU - Turunen, Anna-Mari

AU - Alitalo, Kari

AU - Ylä-Herttala, Seppo

PY - 2000/7

Y1 - 2000/7

N2 - Background: Gene transfer may provide new possibilities for the treat- ment of postangioplasty restenosis. In this study we analyzed the effects of adenovirus-mediated VEGF-C gene transfer on neointima formation after endothelial denudation in rabbits. For comparison, a second group was treated with VEGF-A adenovirus and a third group with lacZ adenovirus. Methods and Results: Aortas of cholesterol-fed New Zealand White rab- bits were balloon denuded and gene transfer was performed three days later. Animals were sacrificed 2 and 4 weeks after the gene transfer and intima/media ratio (I/M), histology and cell proliferation were analyzed. Two weeks after the gene transfer I/M in the lacZ-transfected control group was 0.57 4- 0.04. VEGF-C gene transfer reduced I/M to 0.38 4- 0.02 (P < 0.05 vs. /acZ group). I/M in VEGF-A treated animals was 0.49 =t=0.17 (ns). Expression of VEGF receptors 1, 2 and 3 were detected in the vessel wall by using immunocytochemistry and in situ hybridization. Conclusions: VEGF-C adenovirus gene transfer is effective in reducing in- timal thickening. VEGF-C may be useful for the treatment of postangioplasty restenosis and vessel wall thickening after vascular manipulations.

AB - Background: Gene transfer may provide new possibilities for the treat- ment of postangioplasty restenosis. In this study we analyzed the effects of adenovirus-mediated VEGF-C gene transfer on neointima formation after endothelial denudation in rabbits. For comparison, a second group was treated with VEGF-A adenovirus and a third group with lacZ adenovirus. Methods and Results: Aortas of cholesterol-fed New Zealand White rab- bits were balloon denuded and gene transfer was performed three days later. Animals were sacrificed 2 and 4 weeks after the gene transfer and intima/media ratio (I/M), histology and cell proliferation were analyzed. Two weeks after the gene transfer I/M in the lacZ-transfected control group was 0.57 4- 0.04. VEGF-C gene transfer reduced I/M to 0.38 4- 0.02 (P < 0.05 vs. /acZ group). I/M in VEGF-A treated animals was 0.49 =t=0.17 (ns). Expression of VEGF receptors 1, 2 and 3 were detected in the vessel wall by using immunocytochemistry and in situ hybridization. Conclusions: VEGF-C adenovirus gene transfer is effective in reducing in- timal thickening. VEGF-C may be useful for the treatment of postangioplasty restenosis and vessel wall thickening after vascular manipulations.

U2 - 10.1016/S0021-9150(00)80366-4

DO - 10.1016/S0021-9150(00)80366-4

M3 - Anthology or special issue

VL - 151

T3 - Atherosclerosis

BT - VEGF-C adenovirus gene transfer reduces intima formation in rabbits

ER -

Jeltsch M, Hiltunen MO, Laitinen M, Turunen MP, Hartikainen J, Rissanen TT et al. VEGF-C adenovirus gene transfer reduces intima formation in rabbits. 1 toim. 2000. 1 s. (Atherosclerosis; 1). https://doi.org/10.1016/S0021-9150(00)80366-4