Instruct-HiLIFE CryoEM core facility

CryoEM unit provides high-resolution imaging of nanoparticles, such as virus and protein complexes in the order of 200 KDa upwards (~10 to 300 nm in diameter). CryoEM is particularly useful for objects that are too large, unstable or variable to be studied by X-ray crystallography or NMR. It can be used to understand the structure, assembly and function of various biological macromolecule complexes. The cryoEM data can be used with image processing software to make 3D-models.Training in three-dimensional image reconstruction is given through fortnightly to monthly visits for data processing. Correlative light microscopy and cryoEM is a unique national service.
CryoEM core facility together with Biocomplex, NMR, and protein crystallization core facilities form Instruct-HiLIFE platform of Integrated Structural Cell Biology, which operates both at the Faculty of Biological and Environmental Sciences and Institute of Biotechnology (HiLIFE).