Foto på Katri Eskelin
  • Viikinkaari 9, Biocenter 1, 6th floor, room 6215

    00014 University of Helsinki

    Finland

20002019
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Personlig profil

Information om forskning och undervisning

Novel purification and analysis methods for macromolecular complexes.

Asymmetric flow field-flow fractionation (AF4) is a subtechnique of field-flow fractionation. It separates molecules according to differences in their diffusion coefficients, which in turn reflects their size and shape. AF4 is applicable to a broad range of molecular masses and sizes and molecules with sizes that range from 2 nm to 50 μm can be analyzed.

My research focuses on the development of optimized methods to purify large macromolecular complexes such as ribosomes (a complex of 80 ribosomal proteins and three ribosomal RNAs), viral assemblies as well as intact viruses of various sizes and morphologies. Furthermore, my research aims to use AF4 for detailed analysis of large macromolecular complexes. This can be achieved by analyzing complexes that have been treated with various chemicals, enzymes, temperatures etc.

I am combining the AF4 purification to other methods that include monolithic anion chromatography and centrifugation methods to enable large-scale purification of macromolecular complexes to detailed biochemical and structural analysis.

My previous research included studies on two different plant viruses (Cocksfoot mottle virus and Potato virus A) and applied projects on heterologous protein production in plants.  

Vetenskapsgrenar

  • 1183 Växtbiologi, mikrobiologi, virologi

Internationellt och inhemskt samarbete Publikationer och projekt inom de senaste fem åren.

Publikationer 2000 2019

Öppen tillgång
Fil

Asymmetrical flow field-flow fractionation in purification of an enveloped bacteriophage φ 6

Lampi, M., Oksanen, H. M., Meier, F., Moldenhauer, E., Poranen, M. M., Bamford, D. H. & Eskelin, K., 15 sep 2018, I : Journal of Chromatography. B. 1095, s. 251-257 7 s.

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

Controlled Disassembly and Purification of Functional Viral Subassemblies Using Asymmetrical Flow Field-Flow Fractionation (AF4)

Eskelin, K. J. & Poranen, M. M., nov 2018, I : Viruses. 10, 11, 13 s., 579.

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

Öppen tillgång
Fil

Halophilic viruses with varying biochemical and biophysical properties are amenable to purification with asymmetrical flow field-flow fractionation

Eskelin, K., Lampi, M., Meier, F., Moldenhauer, E., Bamford, D. H. & Oksanen, H. M., nov 2017, I : Extremophiles. 21, 6, s. 1119-1132 14 s.

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

Öppen tillgång
Fil

Asymmetric flow field flow fractionation methods for virus purification

Eskelin, K. J., Lampi, M., Meier, F., Moldenhauer, E., Bamford, D. H. & Oksanen, H. M., 2016, I : Journal of Chromatography. A. 1469, s. 108–119

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

Öppen tillgång
Fil

Projekter 2009 2016

Aktiviteter 2012 2019

Analytica Chimica Acta (Tidskrift)

Katri Eskelin (Referentgranskare)
20 jun 2019

Aktivitet: Typer för peer review av output och redaktionsarbeteReferentgranskning av manuskript

Bioturvakurssi

Katri Eskelin (Närvarande)
19 jun 201820 jun 2018

Aktivitet: Typer för deltagande i eller organisering av evenemangArrangemang av och deltagande i konferens/workshop/kurs/seminarium

Analytical and Bioanalytical Chemistry (Tidskrift)

Katri Eskelin (Referentgranskare)
9 apr 2018

Aktivitet: Typer för peer review av output och redaktionsarbeteReferentgranskning av manuskript

Analytical Chemistry (Tidskrift)

Katri Eskelin (Referentgranskare)
1 okt 2018

Aktivitet: Typer för peer review av output och redaktionsarbeteReferentgranskning av manuskript

Asymmetrical flow field flow fractionation services provided by Instruct-FI Biocomplex unit

Katri Eskelin (!!Speaker)
15 nov 2018

Aktivitet: Typer för tal eller presentation!!Invited talk