Sammanfattning
Copper radical alcohol oxidases belonging to auxiliary activity family 5, subfamily 2 (AA5_2) catalyze the oxidation of galactose and galactosides, as well as aliphatic alcohols. Despite their broad applied potential, so far very few AA5_2 members have been biochemically characterized. We report the recombinant production and biochemical characterization of an AA5_2 oxidase from Penicillium rubens Wisconsin 54-1255 (PruAA5_2A), which groups within an unmapped clade phylogenetically distant from those comprising AA5_2 members characterized to date. PruAA5_2 preferentially oxidized raffinose over galactose; however, its catalytic efficiency was 6.5 times higher on glycolaldehyde dimer compared to raffinose. Deep sequence analysis of characterized AA5_2 members highlighted amino acid pairs correlated to substrate range and conserved within the family. Moreover, PruAA5_2 activity spans substrate preferences previously reported for AA5 subfamily 1 and 2 members, identifying possible functional overlap across the AA5 family.
| Originalspråk | engelska |
|---|---|
| Artikelnummer | 0216546 |
| Tidskrift | PLoS One |
| Volym | 14 |
| Utgåva | 5 |
| Sidor (från-till) | 1-19 |
| Antal sidor | 19 |
| ISSN | 1932-6203 |
| DOI | |
| Status | Publicerad - 15 maj 2019 |
| MoE-publikationstyp | A1 Tidskriftsartikel-refererad |
Vetenskapsgrenar
- 414 Jordbruksbioteknologi
- 1183 Växtbiologi, mikrobiologi, virologi
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A family AA5_2 carbohydrate oxidase from Penicillium rubens displays functional overlap across the AA5 family. / Mollerup, Filip; Aumala, Ville; Parikka, Kirsti Maria; Mathieu, Yan; Brumer, Harry; Tenkanen, Tiina Maija; Master, Emma.
I: PLoS One, Vol. 14, Nr. 5, 0216546, 15.05.2019, s. 1-19.Forskningsoutput: Tidskriftsbidrag › Artikel › Vetenskaplig › Peer review
TY - JOUR
T1 - A family AA5_2 carbohydrate oxidase from Penicillium rubens displays functional overlap across the AA5 family
AU - Mollerup, Filip
AU - Aumala, Ville
AU - Parikka, Kirsti Maria
AU - Mathieu, Yan
AU - Brumer, Harry
AU - Tenkanen, Tiina Maija
AU - Master, Emma
PY - 2019/5/15
Y1 - 2019/5/15
N2 - Copper radical alcohol oxidases belonging to auxiliary activity family 5, subfamily 2 (AA5_2) catalyze the oxidation of galactose and galactosides, as well as aliphatic alcohols. Despite their broad applied potential, so far very few AA5_2 members have been biochemically characterized. We report the recombinant production and biochemical characterization of an AA5_2 oxidase from Penicillium rubens Wisconsin 54-1255 (PruAA5_2A), which groups within an unmapped clade phylogenetically distant from those comprising AA5_2 members characterized to date. PruAA5_2 preferentially oxidized raffinose over galactose; however, its catalytic efficiency was 6.5 times higher on glycolaldehyde dimer compared to raffinose. Deep sequence analysis of characterized AA5_2 members highlighted amino acid pairs correlated to substrate range and conserved within the family. Moreover, PruAA5_2 activity spans substrate preferences previously reported for AA5 subfamily 1 and 2 members, identifying possible functional overlap across the AA5 family.
AB - Copper radical alcohol oxidases belonging to auxiliary activity family 5, subfamily 2 (AA5_2) catalyze the oxidation of galactose and galactosides, as well as aliphatic alcohols. Despite their broad applied potential, so far very few AA5_2 members have been biochemically characterized. We report the recombinant production and biochemical characterization of an AA5_2 oxidase from Penicillium rubens Wisconsin 54-1255 (PruAA5_2A), which groups within an unmapped clade phylogenetically distant from those comprising AA5_2 members characterized to date. PruAA5_2 preferentially oxidized raffinose over galactose; however, its catalytic efficiency was 6.5 times higher on glycolaldehyde dimer compared to raffinose. Deep sequence analysis of characterized AA5_2 members highlighted amino acid pairs correlated to substrate range and conserved within the family. Moreover, PruAA5_2 activity spans substrate preferences previously reported for AA5 subfamily 1 and 2 members, identifying possible functional overlap across the AA5 family.
KW - 414 Agricultural biotechnology
KW - 1183 Plant biology, microbiology, virology
KW - GALACTOSE-OXIDASE
KW - GLYOXAL OXIDASE
KW - BINDING MODULE
KW - ENZYME
KW - EXPRESSION
KW - MECHANISM
KW - OXIDATION
KW - AEROGELS
KW - GENE
U2 - 10.1371/journal.pone.0216546
DO - 10.1371/journal.pone.0216546
M3 - Article
VL - 14
SP - 1
EP - 19
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 5
M1 - 0216546
ER -