A family AA5_2 carbohydrate oxidase from Penicillium rubens displays functional overlap across the AA5 family

Filip Mollerup, Ville Aumala, Kirsti Maria Parikka, Yan Mathieu, Harry Brumer, Tiina Maija Tenkanen, Emma Master

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

Sammanfattning

Copper radical alcohol oxidases belonging to auxiliary activity family 5, subfamily 2 (AA5_2) catalyze the oxidation of galactose and galactosides, as well as aliphatic alcohols. Despite their broad applied potential, so far very few AA5_2 members have been biochemically characterized. We report the recombinant production and biochemical characterization of an AA5_2 oxidase from Penicillium rubens Wisconsin 54-1255 (PruAA5_2A), which groups within an unmapped clade phylogenetically distant from those comprising AA5_2 members characterized to date. PruAA5_2 preferentially oxidized raffinose over galactose; however, its catalytic efficiency was 6.5 times higher on glycolaldehyde dimer compared to raffinose. Deep sequence analysis of characterized AA5_2 members highlighted amino acid pairs correlated to substrate range and conserved within the family. Moreover, PruAA5_2 activity spans substrate preferences previously reported for AA5 subfamily 1 and 2 members, identifying possible functional overlap across the AA5 family.

Originalspråkengelska
Artikelnummer0216546
TidskriftPLoS One
Volym14
Utgåva5
Sidor (från-till)1-19
Antal sidor19
ISSN1932-6203
DOI
StatusPublicerad - 15 maj 2019
MoE-publikationstypA1 Tidskriftsartikel-refererad

Vetenskapsgrenar

  • 414 Jordbruksbioteknologi
  • 1183 Växtbiologi, mikrobiologi, virologi

Citera det här

Mollerup, Filip ; Aumala, Ville ; Parikka, Kirsti Maria ; Mathieu, Yan ; Brumer, Harry ; Tenkanen, Tiina Maija ; Master, Emma. / A family AA5_2 carbohydrate oxidase from Penicillium rubens displays functional overlap across the AA5 family. I: PLoS One. 2019 ; Vol. 14, Nr. 5. s. 1-19.
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title = "A family AA5_2 carbohydrate oxidase from Penicillium rubens displays functional overlap across the AA5 family",
abstract = "Copper radical alcohol oxidases belonging to auxiliary activity family 5, subfamily 2 (AA5_2) catalyze the oxidation of galactose and galactosides, as well as aliphatic alcohols. Despite their broad applied potential, so far very few AA5_2 members have been biochemically characterized. We report the recombinant production and biochemical characterization of an AA5_2 oxidase from Penicillium rubens Wisconsin 54-1255 (PruAA5_2A), which groups within an unmapped clade phylogenetically distant from those comprising AA5_2 members characterized to date. PruAA5_2 preferentially oxidized raffinose over galactose; however, its catalytic efficiency was 6.5 times higher on glycolaldehyde dimer compared to raffinose. Deep sequence analysis of characterized AA5_2 members highlighted amino acid pairs correlated to substrate range and conserved within the family. Moreover, PruAA5_2 activity spans substrate preferences previously reported for AA5 subfamily 1 and 2 members, identifying possible functional overlap across the AA5 family.",
keywords = "414 Agricultural biotechnology, 1183 Plant biology, microbiology, virology, GALACTOSE-OXIDASE, GLYOXAL OXIDASE, BINDING MODULE, ENZYME, EXPRESSION, MECHANISM, OXIDATION, AEROGELS, GENE",
author = "Filip Mollerup and Ville Aumala and Parikka, {Kirsti Maria} and Yan Mathieu and Harry Brumer and Tenkanen, {Tiina Maija} and Emma Master",
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A family AA5_2 carbohydrate oxidase from Penicillium rubens displays functional overlap across the AA5 family. / Mollerup, Filip; Aumala, Ville; Parikka, Kirsti Maria; Mathieu, Yan; Brumer, Harry; Tenkanen, Tiina Maija; Master, Emma.

I: PLoS One, Vol. 14, Nr. 5, 0216546, 15.05.2019, s. 1-19.

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

TY - JOUR

T1 - A family AA5_2 carbohydrate oxidase from Penicillium rubens displays functional overlap across the AA5 family

AU - Mollerup, Filip

AU - Aumala, Ville

AU - Parikka, Kirsti Maria

AU - Mathieu, Yan

AU - Brumer, Harry

AU - Tenkanen, Tiina Maija

AU - Master, Emma

PY - 2019/5/15

Y1 - 2019/5/15

N2 - Copper radical alcohol oxidases belonging to auxiliary activity family 5, subfamily 2 (AA5_2) catalyze the oxidation of galactose and galactosides, as well as aliphatic alcohols. Despite their broad applied potential, so far very few AA5_2 members have been biochemically characterized. We report the recombinant production and biochemical characterization of an AA5_2 oxidase from Penicillium rubens Wisconsin 54-1255 (PruAA5_2A), which groups within an unmapped clade phylogenetically distant from those comprising AA5_2 members characterized to date. PruAA5_2 preferentially oxidized raffinose over galactose; however, its catalytic efficiency was 6.5 times higher on glycolaldehyde dimer compared to raffinose. Deep sequence analysis of characterized AA5_2 members highlighted amino acid pairs correlated to substrate range and conserved within the family. Moreover, PruAA5_2 activity spans substrate preferences previously reported for AA5 subfamily 1 and 2 members, identifying possible functional overlap across the AA5 family.

AB - Copper radical alcohol oxidases belonging to auxiliary activity family 5, subfamily 2 (AA5_2) catalyze the oxidation of galactose and galactosides, as well as aliphatic alcohols. Despite their broad applied potential, so far very few AA5_2 members have been biochemically characterized. We report the recombinant production and biochemical characterization of an AA5_2 oxidase from Penicillium rubens Wisconsin 54-1255 (PruAA5_2A), which groups within an unmapped clade phylogenetically distant from those comprising AA5_2 members characterized to date. PruAA5_2 preferentially oxidized raffinose over galactose; however, its catalytic efficiency was 6.5 times higher on glycolaldehyde dimer compared to raffinose. Deep sequence analysis of characterized AA5_2 members highlighted amino acid pairs correlated to substrate range and conserved within the family. Moreover, PruAA5_2 activity spans substrate preferences previously reported for AA5 subfamily 1 and 2 members, identifying possible functional overlap across the AA5 family.

KW - 414 Agricultural biotechnology

KW - 1183 Plant biology, microbiology, virology

KW - GALACTOSE-OXIDASE

KW - GLYOXAL OXIDASE

KW - BINDING MODULE

KW - ENZYME

KW - EXPRESSION

KW - MECHANISM

KW - OXIDATION

KW - AEROGELS

KW - GENE

U2 - 10.1371/journal.pone.0216546

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