A microfluidic chip for axonal isolation and electrophysiological measurements

Ville Jokinen, Prasanna Sakha, Pia Suvanto, Claudio Rivera Baeza, Sami Franssila, Sari Lauri, Henri Juhani Huttunen

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

Sammanfattning

A microfluidic chip for culturing neurons and spatially isolating axons from somas is presented for use with visually guided whole-cell electrophysiological measurements. A modular design consisting of detachable and re-sealable layers is used to satisfy the requirements of both long-term neuron culturing as well as electrophysiological measurements. Whole cell patch clamp recordings indicate functional viability of neurons with isolated axons. Fluidic isolation was used to achieve asymmetric lentiviral infection of neurons on a single side reservoir. Neurons were asymmetrically infected with lentiviruses expressing the light-activated cationic channel channelrhodopsin-2. Light-evoked excitatory postsynaptic responses were detected by whole cell recordings of neurons on the uninfected side showing functional synaptic connectivity between the two isolated but axonally connected sides of the device.
Originalspråknederländska
Artikelnummer10.1186/1477-3155-11-11
TidskriftJournal of Neuroscience Methods
Volym212
Utgåva2
Sidor (från-till)276-282
Antal sidor7
ISSN0165-0270
DOI
StatusPublicerad - 30 jan 2013
MoE-publikationstypA1 Tidskriftsartikel-refererad

Vetenskapsgrenar

  • 3112 Neurovetenskaper
  • 216 Materialteknik

Citera det här

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abstract = "A microfluidic chip for culturing neurons and spatially isolating axons from somas is presented for use with visually guided whole-cell electrophysiological measurements. A modular design consisting of detachable and re-sealable layers is used to satisfy the requirements of both long-term neuron culturing as well as electrophysiological measurements. Whole cell patch clamp recordings indicate functional viability of neurons with isolated axons. Fluidic isolation was used to achieve asymmetric lentiviral infection of neurons on a single side reservoir. Neurons were asymmetrically infected with lentiviruses expressing the light-activated cationic channel channelrhodopsin-2. Light-evoked excitatory postsynaptic responses were detected by whole cell recordings of neurons on the uninfected side showing functional synaptic connectivity between the two isolated but axonally connected sides of the device.",
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author = "Ville Jokinen and Prasanna Sakha and Pia Suvanto and {Rivera Baeza}, Claudio and Sami Franssila and Sari Lauri and Huttunen, {Henri Juhani}",
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A microfluidic chip for axonal isolation and electrophysiological measurements. / Jokinen, Ville; Sakha, Prasanna; Suvanto, Pia; Rivera Baeza, Claudio; Franssila, Sami; Lauri, Sari; Huttunen, Henri Juhani.

I: Journal of Neuroscience Methods, Vol. 212, Nr. 2, 10.1186/1477-3155-11-11, 30.01.2013, s. 276-282.

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

TY - JOUR

T1 - A microfluidic chip for axonal isolation and electrophysiological measurements

AU - Jokinen, Ville

AU - Sakha, Prasanna

AU - Suvanto, Pia

AU - Rivera Baeza, Claudio

AU - Franssila, Sami

AU - Lauri, Sari

AU - Huttunen, Henri Juhani

PY - 2013/1/30

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N2 - A microfluidic chip for culturing neurons and spatially isolating axons from somas is presented for use with visually guided whole-cell electrophysiological measurements. A modular design consisting of detachable and re-sealable layers is used to satisfy the requirements of both long-term neuron culturing as well as electrophysiological measurements. Whole cell patch clamp recordings indicate functional viability of neurons with isolated axons. Fluidic isolation was used to achieve asymmetric lentiviral infection of neurons on a single side reservoir. Neurons were asymmetrically infected with lentiviruses expressing the light-activated cationic channel channelrhodopsin-2. Light-evoked excitatory postsynaptic responses were detected by whole cell recordings of neurons on the uninfected side showing functional synaptic connectivity between the two isolated but axonally connected sides of the device.

AB - A microfluidic chip for culturing neurons and spatially isolating axons from somas is presented for use with visually guided whole-cell electrophysiological measurements. A modular design consisting of detachable and re-sealable layers is used to satisfy the requirements of both long-term neuron culturing as well as electrophysiological measurements. Whole cell patch clamp recordings indicate functional viability of neurons with isolated axons. Fluidic isolation was used to achieve asymmetric lentiviral infection of neurons on a single side reservoir. Neurons were asymmetrically infected with lentiviruses expressing the light-activated cationic channel channelrhodopsin-2. Light-evoked excitatory postsynaptic responses were detected by whole cell recordings of neurons on the uninfected side showing functional synaptic connectivity between the two isolated but axonally connected sides of the device.

KW - 3112 Neurovetenskaper

KW - 216 Materialteknik

U2 - 10.1016/j.jneumeth.2012.10.013

DO - 10.1016/j.jneumeth.2012.10.013

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