A non-targeted LC-MS metabolic profiling of pregnancy: longitudinal evidence from healthy and pre-eclamptic pregnancies

Tiina Jääskeläinen, Olli Kärkkäinen, Jenna Jokkala, Anton Klåvus, Seppo Heinonen, Seppo Auriola, Marko Lehtonen, FINNPEC Core Invest Grp, Kati Hanhineva, Hannele Laivuori

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Sammanfattning

IntroductionMaternal metabolism changes substantially during pregnancy. However, few studies have used metabolomics technologies to characterize changes across gestation.Objectives and methodsWe applied liquid chromatography-mass spectrometry (LC-MS) based non-targeted metabolomics to determine whether the metabolic profile of serum differs throughout the pregnancy between pre-eclamptic and healthy women in the FINNPEC (Finnish Genetics of Preeclampsia Consortium) Study. Serum samples were available from early and late pregnancy.ResultsProgression of pregnancy had large-scale effects to the serum metabolite profile. Altogether 50 identified metabolites increased and 49 metabolites decreased when samples of early pregnancy were compared to samples of late pregnancy. The metabolic signatures of pregnancy were largely shared in pre-eclamptic and healthy women, only urea, monoacylglyceride 18:1 and glycerophosphocholine were identified to be increased in the pre-eclamptic women when compared to healthy controls.ConclusionsOur study highlights the need of large-scale longitudinal metabolomic studies in non-complicated pregnancies before more detailed understanding of metabolism in adverse outcomes could be provided. Our findings are one of the first steps for a broader metabolic understanding of the physiological changes caused by pregnancy per se.

Originalspråkengelska
Artikelnummer20
TidskriftMetabolomics
Volym17
Antal sidor12
ISSN1573-3882
DOI
StatusPublicerad - 29 jan 2021
MoE-publikationstypA1 Tidskriftsartikel-refererad

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