A simple phenol-free isolation method for high-quality RNA from bilberry

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Sammanfattning

High-quality RNA is required for accurate gene expression and transcriptome analysis. The current methods of RNA extraction from berry fruits are either time-consuming or expensive. To simplify the conventional phenol chloroform based RNA extraction method, we modified the protocol with less steps as well as the removal of the use of phenol. In this protocol, the extraction buffer is composed of hexadecyltrimethyl ammonium bromide (CTAB), polyvinylpyrrolidone (PVP), and Dithiothreitol (DTT). The method facilitates efficient removal of polysaccharides and phenolic compounds from both fruit pulp and fruit peel. Additionally, the protocol is phenol free and less toxic than traditional phenol-containing method. High-quality RNA, with RNA Integrity Number value > 8, isolated by this method is applicable for RNA sequencing and qPCR. Only 3-4 working hours are required for one batch of RNA isolation.

Our method replaces the use of phenol-chloroform with chloroform, making the extraction less toxic.

The bilberry friut RNA is of high-quality and purity with less time input. (C) 2021 The Authors. Published by Elsevier B.V.

Originalspråkengelska
Artikelnummer101481
TidskriftMethodsX
Volym8
Antal sidor5
ISSN2215-0161
DOI
StatusPublicerad - 9 aug. 2021
MoE-publikationstypA1 Tidskriftsartikel-refererad

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  • 11831 Växtbiologi

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