Abnormal cervical cytology is associated with increased nitric oxide release in the uterine cervix

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    Objective. The human uterine cervix is capable of producing nitric oxide (NO). We studied the impact of cytological changes on the release of cervical NO. Design. Population-based case-control study. Setting. City of Helsinki, Finland. Population. Cervical cytology tests and cervical fluid samples were collected in 297 women. Methods. Cervical cytology tests, classified according to Bethesda criteria, were specifically analyzed for changes typically seen in human papillomavirus (HPV) infection, and the level of NO metabolites (NOx) in cervical fluid was assessed by Griess reaction. Main outcome measures. The difference in cervical fluid NOx between normal and abnormal cytology. Results. Cervical cytology was normal in 219 women and abnormal in 78 women. Among women with abnormal cytology there was both a higher detection rate (89% vs. 71%) and a higher concentration of NOx (median 22.5 mol/l, 95% CI 14.6-31.9 vs. 11.0 mol/l, 95% CI 8.0-16.7) compared to women with normal cytology. Age, parity, use of oral contraceptives, phase of the menstrual cycle, or history of miscarriage or termination of early pregnancy were not linked to an increased cervical NOx level. Conclusions. Cervical cell changes (suggestive of HPV infection) are accompanied by an increased release of NO in the human cervix. The significance of this finding remains uncertain, but in theory, increased release of NO could modify the outcome of cervical infection.
    Originalspråkengelska
    TidskriftActa Obstetricia et Gynecologica Scandinavica
    Volym88
    Utgåva9
    Sidor (från-till)417-421
    Antal sidor5
    ISSN0001-6349
    DOI
    StatusPublicerad - 2009
    MoE-publikationstypA1 Tidskriftsartikel-refererad

    Vetenskapsgrenar

    • 312 Klinisk medicin

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    @article{eb020e84290746e3aa4ac16d72031d46,
    title = "Abnormal cervical cytology is associated with increased nitric oxide release in the uterine cervix",
    abstract = "Objective. The human uterine cervix is capable of producing nitric oxide (NO). We studied the impact of cytological changes on the release of cervical NO. Design. Population-based case-control study. Setting. City of Helsinki, Finland. Population. Cervical cytology tests and cervical fluid samples were collected in 297 women. Methods. Cervical cytology tests, classified according to Bethesda criteria, were specifically analyzed for changes typically seen in human papillomavirus (HPV) infection, and the level of NO metabolites (NOx) in cervical fluid was assessed by Griess reaction. Main outcome measures. The difference in cervical fluid NOx between normal and abnormal cytology. Results. Cervical cytology was normal in 219 women and abnormal in 78 women. Among women with abnormal cytology there was both a higher detection rate (89{\%} vs. 71{\%}) and a higher concentration of NOx (median 22.5 mol/l, 95{\%} CI 14.6-31.9 vs. 11.0 mol/l, 95{\%} CI 8.0-16.7) compared to women with normal cytology. Age, parity, use of oral contraceptives, phase of the menstrual cycle, or history of miscarriage or termination of early pregnancy were not linked to an increased cervical NOx level. Conclusions. Cervical cell changes (suggestive of HPV infection) are accompanied by an increased release of NO in the human cervix. The significance of this finding remains uncertain, but in theory, increased release of NO could modify the outcome of cervical infection.",
    keywords = "312 Clinical medicine, Obstetrics and gynecology",
    author = "P{\"a}ivi Rahkola and Tomi Mikkola and Pekka Nieminen and Olavi Ylikorkala and Mervi V{\"a}is{\"a}nen-Tommiska",
    year = "2009",
    doi = "10.1080/00016340902807181PII 909259351",
    language = "English",
    volume = "88",
    pages = "417--421",
    journal = "Acta Obstetricia et Gynecologica Scandinavica",
    issn = "0001-6349",
    publisher = "Wiley",
    number = "9",

    }

    TY - JOUR

    T1 - Abnormal cervical cytology is associated with increased nitric oxide release in the uterine cervix

    AU - Rahkola, Päivi

    AU - Mikkola, Tomi

    AU - Nieminen, Pekka

    AU - Ylikorkala, Olavi

    AU - Väisänen-Tommiska, Mervi

    PY - 2009

    Y1 - 2009

    N2 - Objective. The human uterine cervix is capable of producing nitric oxide (NO). We studied the impact of cytological changes on the release of cervical NO. Design. Population-based case-control study. Setting. City of Helsinki, Finland. Population. Cervical cytology tests and cervical fluid samples were collected in 297 women. Methods. Cervical cytology tests, classified according to Bethesda criteria, were specifically analyzed for changes typically seen in human papillomavirus (HPV) infection, and the level of NO metabolites (NOx) in cervical fluid was assessed by Griess reaction. Main outcome measures. The difference in cervical fluid NOx between normal and abnormal cytology. Results. Cervical cytology was normal in 219 women and abnormal in 78 women. Among women with abnormal cytology there was both a higher detection rate (89% vs. 71%) and a higher concentration of NOx (median 22.5 mol/l, 95% CI 14.6-31.9 vs. 11.0 mol/l, 95% CI 8.0-16.7) compared to women with normal cytology. Age, parity, use of oral contraceptives, phase of the menstrual cycle, or history of miscarriage or termination of early pregnancy were not linked to an increased cervical NOx level. Conclusions. Cervical cell changes (suggestive of HPV infection) are accompanied by an increased release of NO in the human cervix. The significance of this finding remains uncertain, but in theory, increased release of NO could modify the outcome of cervical infection.

    AB - Objective. The human uterine cervix is capable of producing nitric oxide (NO). We studied the impact of cytological changes on the release of cervical NO. Design. Population-based case-control study. Setting. City of Helsinki, Finland. Population. Cervical cytology tests and cervical fluid samples were collected in 297 women. Methods. Cervical cytology tests, classified according to Bethesda criteria, were specifically analyzed for changes typically seen in human papillomavirus (HPV) infection, and the level of NO metabolites (NOx) in cervical fluid was assessed by Griess reaction. Main outcome measures. The difference in cervical fluid NOx between normal and abnormal cytology. Results. Cervical cytology was normal in 219 women and abnormal in 78 women. Among women with abnormal cytology there was both a higher detection rate (89% vs. 71%) and a higher concentration of NOx (median 22.5 mol/l, 95% CI 14.6-31.9 vs. 11.0 mol/l, 95% CI 8.0-16.7) compared to women with normal cytology. Age, parity, use of oral contraceptives, phase of the menstrual cycle, or history of miscarriage or termination of early pregnancy were not linked to an increased cervical NOx level. Conclusions. Cervical cell changes (suggestive of HPV infection) are accompanied by an increased release of NO in the human cervix. The significance of this finding remains uncertain, but in theory, increased release of NO could modify the outcome of cervical infection.

    KW - 312 Clinical medicine

    KW - Obstetrics and gynecology

    U2 - 10.1080/00016340902807181PII 909259351

    DO - 10.1080/00016340902807181PII 909259351

    M3 - Article

    VL - 88

    SP - 417

    EP - 421

    JO - Acta Obstetricia et Gynecologica Scandinavica

    JF - Acta Obstetricia et Gynecologica Scandinavica

    SN - 0001-6349

    IS - 9

    ER -