Absolute and Relative Quantification of Single-Stranded Positive-Sense RNA Viruses from Plant Tissue

Pinky Dutta; Kristiina Mäkinen

doi:10.1007/978-1-0716-3485-1_7

Absolute and Relative Quantification of Single-Stranded Positive-Sense RNA Viruses from Plant Tissue

Forskningsoutput: Kapitel i bok/rapport/konferenshandling › Kapitel › Vetenskaplig › Peer review

Sammanfattning

Reverse transcription quantitative PCR (RT-qPCR) allows sensitive and specific measurement of mRNA transcripts from a given sample in a short period of time. Relative and absolute RT-qPCR are two strategies that could be used to quantify mRNA transcripts, based on the goal of the experiment. Here, we describe the protocol for the quantification of plant viral RNA transcripts from an infected sample using both strategies.

Originalspråk	engelska
Titel på värdpublikation	Plant-Virus Interactions
Redaktörer	Elizabeth P.B. Fontes, Kristiina Mäkinen
Antal sidor	11
Utgivningsort	New York
Förlag	Humana press
Utgivningsdatum	2024
Sidor	81-91
ISBN (tryckt)	978-1-0716-3484-4, 978-1-0716-3487-5
ISBN (elektroniskt)	978-1-0716-3485-1
DOI	https://doi.org/10.1007/978-1-0716-3485-1_7
Status	Publicerad - 2024
MoE-publikationstyp	A3 Del av bok eller annan forskningsbok

Publikationsserier

Namn	Methods in Molecular Biology
Volym	2724
ISSN (tryckt)	1064-3745
ISSN (elektroniskt)	1940-6029

Bibliografisk information

Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.

Vetenskapsgrenar

11831 Växtbiologi
4111 Jordbruksvetenskap

Åtkomst av dokument

10.1007/978-1-0716-3485-1_7Licens: !!Unspecified

Final_draft_Absolute and relative quantification of ssRNA virus-1Accepterat manuskript från författare, 165 KBLicens: !!Unspecified

Citera det här

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Absolute and Relative Quantification of Single-Stranded Positive-Sense RNA Viruses from Plant Tissue. / Dutta, Pinky ; Mäkinen, Kristiina.
Plant-Virus Interactions. red. / Elizabeth P.B. Fontes; Kristiina Mäkinen. New York: Humana press, 2024. s. 81-91 (Methods in Molecular Biology; Vol. 2724).

Forskningsoutput: Kapitel i bok/rapport/konferenshandling › Kapitel › Vetenskaplig › Peer review

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