Acetylated nanocellulose for single-component bioinks and cell proliferation on 3D-printed scaffolds

Rubina Ajdary, Siqi Huan, Nazanin Zanjanizadeh Ezazi, Wenchao Xiang, Rafael Grande, Hélder A. Santos, Orlando J. Rojas

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

Sammanfattning

Nanocellulose has been demonstrated as a suitable material for cell culturing, given its similarity to extracellular matrices. Taking advantage of the shear thinning behavior, nanocellulose suits three-dimensional (3D) printing into scaffolds that support cell attachment and proliferation. Here, we propose aqueous suspensions of acetylated nanocellulose of a low degree of substitution for direct ink writing (DM). This benefits from the heterogeneous acetylation of precursor cellulosic fibers, which eases their deconstruction and confers the characteristics required for extrusion in DIW. Accordingly, the morphology of related 3D printed architectures and their performance during drying and rewetting as well as interactions with living cells are compared with those produced from typical unmodified and TEMPO-oxidized nanocelluloses. We find that a significantly lower concentration of acetylated nanofibrils is needed to obtain bioinks of similar performance, affording more porous structures. Together with their high surface charge and axial aspect, acetylated nanocellulose produces dimensionally stable monolithic scaffolds that support drying and rewetting, required for packaging and sterilization. Considering their potential uses in cardiac devices, we discuss the interactions of the scaffolds with cardiac myoblast cells. Attachment, proliferation, and viability for 21 days are demonstrated. Overall, the performance of acetylated nanocellulose bioinks opens the possibility for reliable and scaleup fabrication of scaffolds appropriate for studies on cellular processes and for tissue engineering.

Originalspråkengelska
TidskriftBiomacromolecules
Volym20
Utgåva7
Sidor (från-till)2770-2778
Antal sidor9
ISSN1525-7797
DOI
StatusPublicerad - jul 2019
MoE-publikationstypA1 Tidskriftsartikel-refererad

Vetenskapsgrenar

  • 1182 Biokemi, cell- och molekylärbiologi
  • 116 Kemi
  • 216 Materialteknik

Citera det här

Ajdary, Rubina ; Huan, Siqi ; Zanjanizadeh Ezazi, Nazanin ; Xiang, Wenchao ; Grande, Rafael ; Santos, Hélder A. ; Rojas, Orlando J. / Acetylated nanocellulose for single-component bioinks and cell proliferation on 3D-printed scaffolds. I: Biomacromolecules. 2019 ; Vol. 20, Nr. 7. s. 2770-2778 .
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abstract = "Nanocellulose has been demonstrated as a suitable material for cell culturing, given its similarity to extracellular matrices. Taking advantage of the shear thinning behavior, nanocellulose suits three-dimensional (3D) printing into scaffolds that support cell attachment and proliferation. Here, we propose aqueous suspensions of acetylated nanocellulose of a low degree of substitution for direct ink writing (DM). This benefits from the heterogeneous acetylation of precursor cellulosic fibers, which eases their deconstruction and confers the characteristics required for extrusion in DIW. Accordingly, the morphology of related 3D printed architectures and their performance during drying and rewetting as well as interactions with living cells are compared with those produced from typical unmodified and TEMPO-oxidized nanocelluloses. We find that a significantly lower concentration of acetylated nanofibrils is needed to obtain bioinks of similar performance, affording more porous structures. Together with their high surface charge and axial aspect, acetylated nanocellulose produces dimensionally stable monolithic scaffolds that support drying and rewetting, required for packaging and sterilization. Considering their potential uses in cardiac devices, we discuss the interactions of the scaffolds with cardiac myoblast cells. Attachment, proliferation, and viability for 21 days are demonstrated. Overall, the performance of acetylated nanocellulose bioinks opens the possibility for reliable and scaleup fabrication of scaffolds appropriate for studies on cellular processes and for tissue engineering.",
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year = "2019",
month = "7",
doi = "10.1021/acs.biomac.9b00527",
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Acetylated nanocellulose for single-component bioinks and cell proliferation on 3D-printed scaffolds. / Ajdary, Rubina; Huan, Siqi; Zanjanizadeh Ezazi, Nazanin; Xiang, Wenchao; Grande, Rafael ; Santos, Hélder A.; Rojas, Orlando J.

I: Biomacromolecules, Vol. 20, Nr. 7, 07.2019, s. 2770-2778 .

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

TY - JOUR

T1 - Acetylated nanocellulose for single-component bioinks and cell proliferation on 3D-printed scaffolds

AU - Ajdary, Rubina

AU - Huan, Siqi

AU - Zanjanizadeh Ezazi, Nazanin

AU - Xiang, Wenchao

AU - Grande, Rafael

AU - Santos, Hélder A.

AU - Rojas, Orlando J.

PY - 2019/7

Y1 - 2019/7

N2 - Nanocellulose has been demonstrated as a suitable material for cell culturing, given its similarity to extracellular matrices. Taking advantage of the shear thinning behavior, nanocellulose suits three-dimensional (3D) printing into scaffolds that support cell attachment and proliferation. Here, we propose aqueous suspensions of acetylated nanocellulose of a low degree of substitution for direct ink writing (DM). This benefits from the heterogeneous acetylation of precursor cellulosic fibers, which eases their deconstruction and confers the characteristics required for extrusion in DIW. Accordingly, the morphology of related 3D printed architectures and their performance during drying and rewetting as well as interactions with living cells are compared with those produced from typical unmodified and TEMPO-oxidized nanocelluloses. We find that a significantly lower concentration of acetylated nanofibrils is needed to obtain bioinks of similar performance, affording more porous structures. Together with their high surface charge and axial aspect, acetylated nanocellulose produces dimensionally stable monolithic scaffolds that support drying and rewetting, required for packaging and sterilization. Considering their potential uses in cardiac devices, we discuss the interactions of the scaffolds with cardiac myoblast cells. Attachment, proliferation, and viability for 21 days are demonstrated. Overall, the performance of acetylated nanocellulose bioinks opens the possibility for reliable and scaleup fabrication of scaffolds appropriate for studies on cellular processes and for tissue engineering.

AB - Nanocellulose has been demonstrated as a suitable material for cell culturing, given its similarity to extracellular matrices. Taking advantage of the shear thinning behavior, nanocellulose suits three-dimensional (3D) printing into scaffolds that support cell attachment and proliferation. Here, we propose aqueous suspensions of acetylated nanocellulose of a low degree of substitution for direct ink writing (DM). This benefits from the heterogeneous acetylation of precursor cellulosic fibers, which eases their deconstruction and confers the characteristics required for extrusion in DIW. Accordingly, the morphology of related 3D printed architectures and their performance during drying and rewetting as well as interactions with living cells are compared with those produced from typical unmodified and TEMPO-oxidized nanocelluloses. We find that a significantly lower concentration of acetylated nanofibrils is needed to obtain bioinks of similar performance, affording more porous structures. Together with their high surface charge and axial aspect, acetylated nanocellulose produces dimensionally stable monolithic scaffolds that support drying and rewetting, required for packaging and sterilization. Considering their potential uses in cardiac devices, we discuss the interactions of the scaffolds with cardiac myoblast cells. Attachment, proliferation, and viability for 21 days are demonstrated. Overall, the performance of acetylated nanocellulose bioinks opens the possibility for reliable and scaleup fabrication of scaffolds appropriate for studies on cellular processes and for tissue engineering.

KW - CELLULOSE NANOFIBRILS

KW - DIFFERENTIATION

KW - HYDROGEL

KW - NANOCRYSTALS

KW - STRENGTH

KW - 1182 Biochemistry, cell and molecular biology

KW - 116 Chemical sciences

KW - 216 Materials engineering

U2 - 10.1021/acs.biomac.9b00527

DO - 10.1021/acs.biomac.9b00527

M3 - Article

VL - 20

SP - 2770

EP - 2778

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 7

ER -