Acidipropionibacterium virtanenii sp. nov., isolated from malted barley

Paulina Deptula, Olli-Pekka Aukusti Smolander, Pia Kati Sofia Laine, Richard J. Roberts, Johanna Minnamari Edelmann, Petri Peltola, Vieno Irene Piironen, Lars Göran Paulin, Erna Storgårds, Kirsi Savijoki, Arja Laitila, Petri Olli Viljami Auvinen, Pekka Kristian Varmanen

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

Sammanfattning

A Gram-stain-positive, catalase-positive and pleomorphic rod organism was isolated from malted barley in Finland, classified initially by partial 16S rRNA gene sequencing and originally deposited in the VTT Culture Collection as a strain of Propionibacterium acidipropionici (currently Acidipropionibacterium acidipropionici). The subsequent comparison of the whole 16S rRNA gene with other representatives of the genus Acidipropionibacterium revealed that the strain belongs to a novel species, most closely related to Acidipropionibacterium microaerophilum and Acidipropionibacterium acidipropionici, with similarity values of 98.46 and 98.31 %, respectively. The whole genome sequencing using PacBio RS II platform allowed further comparison of the genome with all of the other DNA sequences available for the type strains of the Acidipropionibacterium species. Those comparisons revealed the highest similarity of strain JS278(T) to A. acidipropionici, which was confirmed by the average nucleotide identity analysis. The genome of strain JS278(T) is intermediate in size compared to the A. acidipropionici and Acidipropionibacterium jensenii at 3 432 872 bp, the G+C content is 68.4 mol%. The strain fermented a wide range of carbon sources, and produced propionic acid as the major fermentation product. Besides its poor ability to grow at 37 degrees C and positive catalase reaction, the observed phenotype was almost indistinguishable from those of A. acidipropionici and A. jensenii. Based on our findings, we conclude that the organism represents a novel member of the genus Acidipropionibacterium, for which we propose the name Acidipropionibacterium virtanenii sp. nov. The type strain is JS278(T) (=VTT E-113202(T)=DSM 106790(T)).

Originalspråkengelska
TidskriftInternational Journal of Systematic and Evolutionary Microbiology
Volym68
Nummer10
Sidor (från-till)3175-3183
Antal sidor9
ISSN1466-5026
DOI
StatusPublicerad - okt. 2018
MoE-publikationstypA1 Tidskriftsartikel-refererad

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  • 1183 Växtbiologi, mikrobiologi, virologi

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