Anticancer and immunomodulatory activities of a novel water-soluble derivative of Ellipticine

Regiane Costa de Oliveira, Gemilson Soares Pontes, Aleksandr Kostyuk, Gabriel B. Coutinho Camargo, Anamika Dhyani, Tetiana Shvydenko, Kostiantyn Shvydenko, Andriy Grafov

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

Sammanfattning

Cancer still remains a major public health concern around the world and the search for new potential antitumor molecules is essential for fighting the disease. This study evaluated the anticancer and immunomodulatory potential of the newly synthetized ellipticine derivate: sodium bromo-5,11-dimethyl-6H-pyrido[4,3-b]carbazole-7-sulfonate (Br-Ell-SO3Na). It was prepared by the
chlorosulfonation of 9-bromoellipticine. The ellipticine-7-sulfonic acid itself is not soluble, but its saponification with sodium hydroxide afforded a water-soluble sodium salt. The cytotoxicity of Br-Ell-SO3Na was tested against cancerous (K562 cell line) and non-cancerous cells (Vero cell line and
human peripheral blood mononuclear cells (PBMC)) using a Methylthiazoletetrazolium (MTT) assay. Cell cycle arrest was assessed by flow cytometry and the immunomodulatory activity was analyzed through an enzyme-linked immunosorbent assay (ELISA). The results showed that the Br-Ell-SO3Na molecule has specific anticancer activity (IC50 = 35 µM) against the K562 cell line, once no cytotoxicity effect was verified against non-cancerous cells. Cell cycle analysis demonstrated that K562 cells treated with Br-Ell-SO3Na were arrested in the phase S. Moreover, the production of IL-6 increased and the expression of IL-8 was inhibited in the human PBMC treated with Br-Ell-SO3Na. The results demonstrated that Br-Ell-SO3Na is a promising anticancer molecule attested by its noteworthy activity against the K562 tumor cell line and immunomodulatory activity in human PBMC cells.
Originalspråkengelska
Artikelnummer2130
TidskriftMolecules
Volym25
Utgåva9
Antal sidor12
ISSN1420-3049
DOI
StatusPublicerad - maj 2020
MoE-publikationstypA1 Tidskriftsartikel-refererad

Vetenskapsgrenar

  • 1182 Biokemi, cell- och molekylärbiologi
  • 116 Kemi

Citera det här

Costa de Oliveira, R., Soares Pontes, G., Kostyuk, A., Coutinho Camargo, G. B., Dhyani, A., Shvydenko, T., Shvydenko, K., & Grafov, A. (2020). Anticancer and immunomodulatory activities of a novel water-soluble derivative of Ellipticine. Molecules, 25(9), [2130]. https://doi.org/10.3390/molecules25092130