B lymphopoiesis is characterized by pre-B cell marker gene expression in fetal cattle and declines in adults

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Sammanfattning

Fetal cattle B-cell development proceeds via a pre-B cell stage that is
characterized by the expression of surrogate light chain and recombination
activation genes. In this paper, we identify a new member of bovine pre-B
lymphocyte genes, VPREB2. Using RT-qPCR, we assess the expression of VPREB2 and
three other surrogate light chain genes as well as RAG1 and RAG2 in fetal and
adult cattle tissues. The absence of VPREB1, IGLL1, RAG1 and RAG2 expression in
adult tissues and the lack of B-lymphoid differentiation in adult bone marrow -
OP9 stromal cell co-culture, suggest a decline of B lymphopoiesis in adult
cattle. The marked differences in the expression profiles of VPREB2 and VPREB3 in
comparison to those of VPREB1, IGLL1 and RAGs suggest that the biological roles
of VPREB2 and VPREB3 are unrelated to the pre-B cells.
Originalspråkengelska
TidskriftDevelopmental & Comparative Immunology
Volym37
Utgåva1
Sidor (från-till)39-49
Antal sidor11
ISSN0145-305X
DOI
StatusPublicerad - 2012
MoE-publikationstypA1 Tidskriftsartikel-refererad

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  • 1184 Genetik, utvecklingsbiologi, fysiologi
  • 413 Veterinärvetenskap

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