Biomimetic engineering using cancer cell membranes for designing compartmentalized nanoreactors with organelle-like functions

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Sammanfattning

Here, we have created a new biomimetic nanoreactor consisting of porous silicon
nanoparticles providing the favourable microenvironment for entrapped horseradish peroxidase enzyme and isolated cancer cell membrane materials featuring enclosed membrane, in order to mimic the cellular compartmentalization strategy. The enzyme activities and kinetics analyses showed enhanced substrate affinities and reaction rates compared to free enzymes, suggesting a high catalytic activity of the nanoreactors. The in vitro cell experiments demonstrated that the nanoreactors are cytocompatible, readily integrated with cells, while maintaining the intactness of the structure and being intracellularly stable. The intracellular activity of the nanoreactors in the stimulated oxidative stress conditions demonstrated the fact that the nanoreactors could significantly reduce the intracellular reactive oxygen species levels and supplement the sub-cellular organelles functions. Therefore, the developed biomimetic nanoreactors can function as artificial organelles inside cells to counteract the oxidative stress that is involved in an array of human diseases. Overall, this cellular nanoreactor featuring a biocompartment enclosed by a cellular membrane closely resembles nature's biocompartmentalization opens a new avenue for biomimetic nanoreactor design for the further development of customized biomimetic nanomachineries using different cell types.
Originalspråkengelska
Artikelnummer1605375
TidskriftAdvanced Materials
Volym29
Utgåva11
Antal sidor8
ISSN0935-9648
DOI
StatusPublicerad - 21 mar 2017
MoE-publikationstypA1 Tidskriftsartikel-refererad

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  • 116 Kemi
  • 221 Nanoteknologi
  • 317 Farmaci

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title = "Biomimetic engineering using cancer cell membranes for designing compartmentalized nanoreactors with organelle-like functions",
abstract = "Here, we have created a new biomimetic nanoreactor consisting of porous siliconnanoparticles providing the favourable microenvironment for entrapped horseradish peroxidase enzyme and isolated cancer cell membrane materials featuring enclosed membrane, in order to mimic the cellular compartmentalization strategy. The enzyme activities and kinetics analyses showed enhanced substrate affinities and reaction rates compared to free enzymes, suggesting a high catalytic activity of the nanoreactors. The in vitro cell experiments demonstrated that the nanoreactors are cytocompatible, readily integrated with cells, while maintaining the intactness of the structure and being intracellularly stable. The intracellular activity of the nanoreactors in the stimulated oxidative stress conditions demonstrated the fact that the nanoreactors could significantly reduce the intracellular reactive oxygen species levels and supplement the sub-cellular organelles functions. Therefore, the developed biomimetic nanoreactors can function as artificial organelles inside cells to counteract the oxidative stress that is involved in an array of human diseases. Overall, this cellular nanoreactor featuring a biocompartment enclosed by a cellular membrane closely resembles nature's biocompartmentalization opens a new avenue for biomimetic nanoreactor design for the further development of customized biomimetic nanomachineries using different cell types.",
keywords = "116 Chemical sciences, 221 Nano-technology, 317 Pharmacy, POROUS SILICON NANOPARTICLES , BIOMEDICAL APPLICATIONS , SURFACE-CHEMISTRY , HORSERADISH-PEROXIDASE , COATED NANOPARTICLES , ENZYMATIC-REACTIONS , MESOPOROUS SILICA , DRUG-DELIVERY , IN-VITRO , MICROPARTICLES",
author = "Vimalkumar Balasubramanian and {Rebelo Correia}, {Alexandra Maria} and Hongbo Zhang and Flavia Fontana and Ermei M{\"a}kil{\"a} and Jarno Salonen and Hirvonen, {Jouni Tapio} and {Almeida Santos}, Helder",
year = "2017",
month = "3",
day = "21",
doi = "10.1002/adma.201605375",
language = "English",
volume = "29",
journal = "Advanced Materials",
issn = "0935-9648",
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number = "11",

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TY - JOUR

T1 - Biomimetic engineering using cancer cell membranes for designing compartmentalized nanoreactors with organelle-like functions

AU - Balasubramanian, Vimalkumar

AU - Rebelo Correia, Alexandra Maria

AU - Zhang, Hongbo

AU - Fontana, Flavia

AU - Mäkilä, Ermei

AU - Salonen, Jarno

AU - Hirvonen, Jouni Tapio

AU - Almeida Santos, Helder

PY - 2017/3/21

Y1 - 2017/3/21

N2 - Here, we have created a new biomimetic nanoreactor consisting of porous siliconnanoparticles providing the favourable microenvironment for entrapped horseradish peroxidase enzyme and isolated cancer cell membrane materials featuring enclosed membrane, in order to mimic the cellular compartmentalization strategy. The enzyme activities and kinetics analyses showed enhanced substrate affinities and reaction rates compared to free enzymes, suggesting a high catalytic activity of the nanoreactors. The in vitro cell experiments demonstrated that the nanoreactors are cytocompatible, readily integrated with cells, while maintaining the intactness of the structure and being intracellularly stable. The intracellular activity of the nanoreactors in the stimulated oxidative stress conditions demonstrated the fact that the nanoreactors could significantly reduce the intracellular reactive oxygen species levels and supplement the sub-cellular organelles functions. Therefore, the developed biomimetic nanoreactors can function as artificial organelles inside cells to counteract the oxidative stress that is involved in an array of human diseases. Overall, this cellular nanoreactor featuring a biocompartment enclosed by a cellular membrane closely resembles nature's biocompartmentalization opens a new avenue for biomimetic nanoreactor design for the further development of customized biomimetic nanomachineries using different cell types.

AB - Here, we have created a new biomimetic nanoreactor consisting of porous siliconnanoparticles providing the favourable microenvironment for entrapped horseradish peroxidase enzyme and isolated cancer cell membrane materials featuring enclosed membrane, in order to mimic the cellular compartmentalization strategy. The enzyme activities and kinetics analyses showed enhanced substrate affinities and reaction rates compared to free enzymes, suggesting a high catalytic activity of the nanoreactors. The in vitro cell experiments demonstrated that the nanoreactors are cytocompatible, readily integrated with cells, while maintaining the intactness of the structure and being intracellularly stable. The intracellular activity of the nanoreactors in the stimulated oxidative stress conditions demonstrated the fact that the nanoreactors could significantly reduce the intracellular reactive oxygen species levels and supplement the sub-cellular organelles functions. Therefore, the developed biomimetic nanoreactors can function as artificial organelles inside cells to counteract the oxidative stress that is involved in an array of human diseases. Overall, this cellular nanoreactor featuring a biocompartment enclosed by a cellular membrane closely resembles nature's biocompartmentalization opens a new avenue for biomimetic nanoreactor design for the further development of customized biomimetic nanomachineries using different cell types.

KW - 116 Chemical sciences

KW - 221 Nano-technology

KW - 317 Pharmacy

KW - POROUS SILICON NANOPARTICLES

KW - BIOMEDICAL APPLICATIONS

KW - SURFACE-CHEMISTRY

KW - HORSERADISH-PEROXIDASE

KW - COATED NANOPARTICLES

KW - ENZYMATIC-REACTIONS

KW - MESOPOROUS SILICA

KW - DRUG-DELIVERY

KW - IN-VITRO

KW - MICROPARTICLES

UR - http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1521-4095

U2 - 10.1002/adma.201605375

DO - 10.1002/adma.201605375

M3 - Article

VL - 29

JO - Advanced Materials

JF - Advanced Materials

SN - 0935-9648

IS - 11

M1 - 1605375

ER -