Chapter 3.1 – Cell-based in vitro models for buccal permeability studies (Book Chapter)

Neha Shrestha, Francisca Araújo, Bruno Sarmento, Jouni Tapio Hirvonen, Helder Almeida Santos

Forskningsoutput: Kapitel i bok/rapport/konferenshandlingKapitelVetenskapligPeer review

Sammanfattning

The buccal route for drug administration has gained importance in the past years due to its accessibility and rapid onset of action. It has been described as a reliable alternative to the oral route, both for local and for systemic delivery of drugs, overcoming some of the drawbacks existent in oral administration, such as the harsh milieu along the gastrointestinal tract. To successfully develop active drugs, the prediction of their absorption profile across the buccal mucosa is of utmost importance. Due to the high costs, time-consuming nature, and ethical considerations of ex vivo and in vivo studies, in vitro models have been the first choice for the screening of the drugs' permeability. Among the several cell lines originating in the buccal mucosa of humans or animals, hamster cheek pouch cells, oral keratinocytes, and TR146 cells have been the most used for permeability studies. However, due to their primary nature, hamster cheek pouch cells can only be used for a low number of tests, which leads to a high variability in the results. Also, the cancerous origin of TR146 makes it less close to human buccal epithelium, as compared to oral keratinocytes derived from human sources, for permeability studies.
Originalspråkengelska
Titel på gästpublikationConcepts and Models for Drug Permeability Studies: Cell and Tissue Based in Vitro Culture Models
RedaktörerBruno Sarmento
Antal sidor10
UtgivningsortAmsterdam
FörlagElsevier Scientific Publ. Co
Utgivningsdatumjan 2016
Sidor31-40
ArtikelnummerChapter 3
ISBN (tryckt)978-0-08-100094-6
ISBN (elektroniskt)978-0-08-100114-1
StatusPublicerad - jan 2016
MoE-publikationstypA3 Del av bok eller annan forskningsbok

Publikationsserier

NamnWoodhead Publishing series in biomedicine
FörlagElsevier
Nummer79

Vetenskapsgrenar

  • 317 Farmaci

Citera det här

Shrestha, N., Araújo, F., Sarmento, B., Hirvonen, J. T., & Almeida Santos, H. (2016). Chapter 3.1 – Cell-based in vitro models for buccal permeability studies (Book Chapter). I B. Sarmento (Red.), Concepts and Models for Drug Permeability Studies: Cell and Tissue Based in Vitro Culture Models (s. 31-40). [Chapter 3] (Woodhead Publishing series in biomedicine; Nr. 79). Amsterdam: Elsevier Scientific Publ. Co.
Shrestha, Neha ; Araújo, Francisca ; Sarmento, Bruno ; Hirvonen, Jouni Tapio ; Almeida Santos, Helder. / Chapter 3.1 – Cell-based in vitro models for buccal permeability studies (Book Chapter). Concepts and Models for Drug Permeability Studies: Cell and Tissue Based in Vitro Culture Models. redaktör / Bruno Sarmento. Amsterdam : Elsevier Scientific Publ. Co, 2016. s. 31-40 (Woodhead Publishing series in biomedicine; 79).
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Shrestha, N, Araújo, F, Sarmento, B, Hirvonen, JT & Almeida Santos, H 2016, Chapter 3.1 – Cell-based in vitro models for buccal permeability studies (Book Chapter). i B Sarmento (red.), Concepts and Models for Drug Permeability Studies: Cell and Tissue Based in Vitro Culture Models., Chapter 3, Woodhead Publishing series in biomedicine, nr. 79, Elsevier Scientific Publ. Co, Amsterdam, s. 31-40.

Chapter 3.1 – Cell-based in vitro models for buccal permeability studies (Book Chapter). / Shrestha, Neha; Araújo, Francisca; Sarmento, Bruno; Hirvonen, Jouni Tapio; Almeida Santos, Helder.

Concepts and Models for Drug Permeability Studies: Cell and Tissue Based in Vitro Culture Models. red. / Bruno Sarmento. Amsterdam : Elsevier Scientific Publ. Co, 2016. s. 31-40 Chapter 3 (Woodhead Publishing series in biomedicine; Nr. 79).

Forskningsoutput: Kapitel i bok/rapport/konferenshandlingKapitelVetenskapligPeer review

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AU - Shrestha, Neha

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AU - Sarmento, Bruno

AU - Hirvonen, Jouni Tapio

AU - Almeida Santos, Helder

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N2 - The buccal route for drug administration has gained importance in the past years due to its accessibility and rapid onset of action. It has been described as a reliable alternative to the oral route, both for local and for systemic delivery of drugs, overcoming some of the drawbacks existent in oral administration, such as the harsh milieu along the gastrointestinal tract. To successfully develop active drugs, the prediction of their absorption profile across the buccal mucosa is of utmost importance. Due to the high costs, time-consuming nature, and ethical considerations of ex vivo and in vivo studies, in vitro models have been the first choice for the screening of the drugs' permeability. Among the several cell lines originating in the buccal mucosa of humans or animals, hamster cheek pouch cells, oral keratinocytes, and TR146 cells have been the most used for permeability studies. However, due to their primary nature, hamster cheek pouch cells can only be used for a low number of tests, which leads to a high variability in the results. Also, the cancerous origin of TR146 makes it less close to human buccal epithelium, as compared to oral keratinocytes derived from human sources, for permeability studies.

AB - The buccal route for drug administration has gained importance in the past years due to its accessibility and rapid onset of action. It has been described as a reliable alternative to the oral route, both for local and for systemic delivery of drugs, overcoming some of the drawbacks existent in oral administration, such as the harsh milieu along the gastrointestinal tract. To successfully develop active drugs, the prediction of their absorption profile across the buccal mucosa is of utmost importance. Due to the high costs, time-consuming nature, and ethical considerations of ex vivo and in vivo studies, in vitro models have been the first choice for the screening of the drugs' permeability. Among the several cell lines originating in the buccal mucosa of humans or animals, hamster cheek pouch cells, oral keratinocytes, and TR146 cells have been the most used for permeability studies. However, due to their primary nature, hamster cheek pouch cells can only be used for a low number of tests, which leads to a high variability in the results. Also, the cancerous origin of TR146 makes it less close to human buccal epithelium, as compared to oral keratinocytes derived from human sources, for permeability studies.

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PB - Elsevier Scientific Publ. Co

CY - Amsterdam

ER -

Shrestha N, Araújo F, Sarmento B, Hirvonen JT, Almeida Santos H. Chapter 3.1 – Cell-based in vitro models for buccal permeability studies (Book Chapter). I Sarmento B, redaktör, Concepts and Models for Drug Permeability Studies: Cell and Tissue Based in Vitro Culture Models. Amsterdam: Elsevier Scientific Publ. Co. 2016. s. 31-40. Chapter 3. (Woodhead Publishing series in biomedicine; 79).