Sammanfattning

Anogeissus leiocarpus occurs in savannas in tropical and subtropical regions of Africa and is used among traditional medicinal practitioners for treatment of various diseases, among them bacterial infections [1]. Eight flavonoids [2], methyl ellagic acid, ellagic acid [4, 5] and the ellagitannins castalagin and flagallonic acid [6] have been reported from the leaves and bark of A. leiocarpus. There are just a few investigations on the phytochemistry of the roots [1], however. This study has aimed on in depth investigations on the phytochemistry of the root part. Ten microliters of 50 mg/ml methanol extract of the root of A. leiocarpus was applied on RP18 TLC reversed phase. Methanol, water and orthophosphoric acid were used as mobile phase. DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent was used for detecting antioxidative compounds. In order to identify these compounds HPLC-DAD and UHPLC/MS/QTOF methods [3] were used. Aromadendrin, taxifolin, methyltaxifolin, ampelopsin and eriodictyol as well as pinosylvin and methylpinosylvin and 3,3´-Di-O-methyl ellagic acid glucoside were identified for the first time in the roots of A. leiocarpus. In addition pentagalloyglucose and digalloylglucose were found in the root. We have demonstrated that a methanolic root extract of A. leiocarpus gives promising antibacterial effects. These effects might be connected to antioxidative flavonoids and ellagic acid derivatives in this extract
Originalspråkengelska
Titel på gästpublikationPlanta Medica
Volym81 - PM_77
FörlagGeorg Thieme Verlag
Utgivningsdatum2015
DOI
StatusPublicerad - 2015
MoE-publikationstypA4 Artikel i en konferenspublikation

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@inproceedings{55eab7f3dee54c42994368c911017114,
title = "Characterization of antibacterial flavonoids and stilbenes of the root extract of Anogeissus leiocarpus by UHPLC-MS-QTOF",
abstract = "Anogeissus leiocarpus occurs in savannas in tropical and subtropical regions of Africa and is used among traditional medicinal practitioners for treatment of various diseases, among them bacterial infections [1]. Eight flavonoids [2], methyl ellagic acid, ellagic acid [4, 5] and the ellagitannins castalagin and flagallonic acid [6] have been reported from the leaves and bark of A. leiocarpus. There are just a few investigations on the phytochemistry of the roots [1], however. This study has aimed on in depth investigations on the phytochemistry of the root part. Ten microliters of 50 mg/ml methanol extract of the root of A. leiocarpus was applied on RP18 TLC reversed phase. Methanol, water and orthophosphoric acid were used as mobile phase. DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent was used for detecting antioxidative compounds. In order to identify these compounds HPLC-DAD and UHPLC/MS/QTOF methods [3] were used. Aromadendrin, taxifolin, methyltaxifolin, ampelopsin and eriodictyol as well as pinosylvin and methylpinosylvin and 3,3´-Di-O-methyl ellagic acid glucoside were identified for the first time in the roots of A. leiocarpus. In addition pentagalloyglucose and digalloylglucose were found in the root. We have demonstrated that a methanolic root extract of A. leiocarpus gives promising antibacterial effects. These effects might be connected to antioxidative flavonoids and ellagic acid derivatives in this extract",
author = "Salih, {Enass Yousif Abdelkarium} and Kanninen, {Markku Tapani} and Riitta Julkunen-Tiitto and Sipi, {Marketta Hannele} and Luukkanen, {Martti Olavi} and Vuorela, {Heikki Juhani} and Fyhrqvist, {Pia Johanna}",
note = "Volume: Proceeding volume: 81 - PM_77",
year = "2015",
doi = "10.1055/s-0035-1565454",
language = "English",
volume = "81 - PM_77",
booktitle = "Planta Medica",
publisher = "Georg Thieme Verlag",
address = "Germany",

}

Characterization of antibacterial flavonoids and stilbenes of the root extract of Anogeissus leiocarpus by UHPLC-MS-QTOF. / Salih, Enass Yousif Abdelkarium; Kanninen, Markku Tapani; Julkunen-Tiitto, Riitta; Sipi, Marketta Hannele; Luukkanen, Martti Olavi; Vuorela, Heikki Juhani; Fyhrqvist, Pia Johanna.

Planta Medica. Vol. 81 - PM_77 Georg Thieme Verlag, 2015.

Forskningsoutput: Kapitel i bok/rapport/konferenshandlingKonferensbidragVetenskapligPeer review

TY - GEN

T1 - Characterization of antibacterial flavonoids and stilbenes of the root extract of Anogeissus leiocarpus by UHPLC-MS-QTOF

AU - Salih, Enass Yousif Abdelkarium

AU - Kanninen, Markku Tapani

AU - Julkunen-Tiitto, Riitta

AU - Sipi, Marketta Hannele

AU - Luukkanen, Martti Olavi

AU - Vuorela, Heikki Juhani

AU - Fyhrqvist, Pia Johanna

N1 - Volume: Proceeding volume: 81 - PM_77

PY - 2015

Y1 - 2015

N2 - Anogeissus leiocarpus occurs in savannas in tropical and subtropical regions of Africa and is used among traditional medicinal practitioners for treatment of various diseases, among them bacterial infections [1]. Eight flavonoids [2], methyl ellagic acid, ellagic acid [4, 5] and the ellagitannins castalagin and flagallonic acid [6] have been reported from the leaves and bark of A. leiocarpus. There are just a few investigations on the phytochemistry of the roots [1], however. This study has aimed on in depth investigations on the phytochemistry of the root part. Ten microliters of 50 mg/ml methanol extract of the root of A. leiocarpus was applied on RP18 TLC reversed phase. Methanol, water and orthophosphoric acid were used as mobile phase. DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent was used for detecting antioxidative compounds. In order to identify these compounds HPLC-DAD and UHPLC/MS/QTOF methods [3] were used. Aromadendrin, taxifolin, methyltaxifolin, ampelopsin and eriodictyol as well as pinosylvin and methylpinosylvin and 3,3´-Di-O-methyl ellagic acid glucoside were identified for the first time in the roots of A. leiocarpus. In addition pentagalloyglucose and digalloylglucose were found in the root. We have demonstrated that a methanolic root extract of A. leiocarpus gives promising antibacterial effects. These effects might be connected to antioxidative flavonoids and ellagic acid derivatives in this extract

AB - Anogeissus leiocarpus occurs in savannas in tropical and subtropical regions of Africa and is used among traditional medicinal practitioners for treatment of various diseases, among them bacterial infections [1]. Eight flavonoids [2], methyl ellagic acid, ellagic acid [4, 5] and the ellagitannins castalagin and flagallonic acid [6] have been reported from the leaves and bark of A. leiocarpus. There are just a few investigations on the phytochemistry of the roots [1], however. This study has aimed on in depth investigations on the phytochemistry of the root part. Ten microliters of 50 mg/ml methanol extract of the root of A. leiocarpus was applied on RP18 TLC reversed phase. Methanol, water and orthophosphoric acid were used as mobile phase. DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent was used for detecting antioxidative compounds. In order to identify these compounds HPLC-DAD and UHPLC/MS/QTOF methods [3] were used. Aromadendrin, taxifolin, methyltaxifolin, ampelopsin and eriodictyol as well as pinosylvin and methylpinosylvin and 3,3´-Di-O-methyl ellagic acid glucoside were identified for the first time in the roots of A. leiocarpus. In addition pentagalloyglucose and digalloylglucose were found in the root. We have demonstrated that a methanolic root extract of A. leiocarpus gives promising antibacterial effects. These effects might be connected to antioxidative flavonoids and ellagic acid derivatives in this extract

UR - https://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-0035-1565454

U2 - 10.1055/s-0035-1565454

DO - 10.1055/s-0035-1565454

M3 - Conference contribution

VL - 81 - PM_77

BT - Planta Medica

PB - Georg Thieme Verlag

ER -