Chondrogenic potential of mouse calvarial mesenchyme

    Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

    Sammanfattning

    "Facial and calvarial bones form intramembranously without a cartilagenous model; however, cultured chick calvarial mesenchyme cells may differentiate into both osteololasts and chondroblasts and, in rodents, small cartilages occasionally form at the sutures in vivo. Therefore, we wanted to investigate what factors regulate normal differentiation of calvarial mesenchymal cells directly into osteoblasts. In embryonic mouse heads and in cultured tissue explants, we analyzed the expression of selected transcription factors and extracellular matrix molecules associated with bone and cartilage development. Cartilage markers Sox9 and type II collagen were expressed in all craniofacial cartilages. In addition, Msx2 and type I collagen were expressed in sense capsule cartilages. We also observed that the undifferentiated calvarial mesenchyme and the osteogenic fronts in the jaw expressed Col2A1. Moreover, we found that cultured mouse calvarial mesenchyme could develop into cartilage. Of the 49 explants that contained mesenchyme, intramembranous ossification occurred in 35%. Only cartilage formed in 4%, and both cartilage and bone formed in 4%. Our study confirms that calvarial mesenchyme, which normally gives rise to intramembranous bone, also has chondrogenic potential."
    Originalspråkengelska
    TidskriftJournal of Histochemistry and Cytochemistry
    Volym53
    Utgåva5
    Sidor (från-till)653-663
    Antal sidor11
    ISSN0022-1554
    DOI
    StatusPublicerad - 2005
    MoE-publikationstypA1 Tidskriftsartikel-refererad

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    @article{a96760b7493547e8879c33b0d230c51b,
    title = "Chondrogenic potential of mouse calvarial mesenchyme",
    abstract = "{"}Facial and calvarial bones form intramembranously without a cartilagenous model; however, cultured chick calvarial mesenchyme cells may differentiate into both osteololasts and chondroblasts and, in rodents, small cartilages occasionally form at the sutures in vivo. Therefore, we wanted to investigate what factors regulate normal differentiation of calvarial mesenchymal cells directly into osteoblasts. In embryonic mouse heads and in cultured tissue explants, we analyzed the expression of selected transcription factors and extracellular matrix molecules associated with bone and cartilage development. Cartilage markers Sox9 and type II collagen were expressed in all craniofacial cartilages. In addition, Msx2 and type I collagen were expressed in sense capsule cartilages. We also observed that the undifferentiated calvarial mesenchyme and the osteogenic fronts in the jaw expressed Col2A1. Moreover, we found that cultured mouse calvarial mesenchyme could develop into cartilage. Of the 49 explants that contained mesenchyme, intramembranous ossification occurred in 35{\%}. Only cartilage formed in 4{\%}, and both cartilage and bone formed in 4{\%}. Our study confirms that calvarial mesenchyme, which normally gives rise to intramembranous bone, also has chondrogenic potential.{"}",
    author = "Thomas {\AA}berg and Ritva Rice and Rice, {David Paul Cracroft} and Irma Thesleff and Waltimo-Siren, {Janna C}",
    year = "2005",
    doi = "10.1369/jhc.4A6518.2005",
    language = "English",
    volume = "53",
    pages = "653--663",
    journal = "Journal of Histochemistry and Cytochemistry",
    issn = "0022-1554",
    publisher = "HISTOCHEMICAL SOC INC",
    number = "5",

    }

    Chondrogenic potential of mouse calvarial mesenchyme. / Åberg, Thomas; Rice, Ritva; Rice, David Paul Cracroft; Thesleff, Irma; Waltimo-Siren, Janna C.

    I: Journal of Histochemistry and Cytochemistry, Vol. 53, Nr. 5, 2005, s. 653-663.

    Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

    TY - JOUR

    T1 - Chondrogenic potential of mouse calvarial mesenchyme

    AU - Åberg, Thomas

    AU - Rice, Ritva

    AU - Rice, David Paul Cracroft

    AU - Thesleff, Irma

    AU - Waltimo-Siren, Janna C

    PY - 2005

    Y1 - 2005

    N2 - "Facial and calvarial bones form intramembranously without a cartilagenous model; however, cultured chick calvarial mesenchyme cells may differentiate into both osteololasts and chondroblasts and, in rodents, small cartilages occasionally form at the sutures in vivo. Therefore, we wanted to investigate what factors regulate normal differentiation of calvarial mesenchymal cells directly into osteoblasts. In embryonic mouse heads and in cultured tissue explants, we analyzed the expression of selected transcription factors and extracellular matrix molecules associated with bone and cartilage development. Cartilage markers Sox9 and type II collagen were expressed in all craniofacial cartilages. In addition, Msx2 and type I collagen were expressed in sense capsule cartilages. We also observed that the undifferentiated calvarial mesenchyme and the osteogenic fronts in the jaw expressed Col2A1. Moreover, we found that cultured mouse calvarial mesenchyme could develop into cartilage. Of the 49 explants that contained mesenchyme, intramembranous ossification occurred in 35%. Only cartilage formed in 4%, and both cartilage and bone formed in 4%. Our study confirms that calvarial mesenchyme, which normally gives rise to intramembranous bone, also has chondrogenic potential."

    AB - "Facial and calvarial bones form intramembranously without a cartilagenous model; however, cultured chick calvarial mesenchyme cells may differentiate into both osteololasts and chondroblasts and, in rodents, small cartilages occasionally form at the sutures in vivo. Therefore, we wanted to investigate what factors regulate normal differentiation of calvarial mesenchymal cells directly into osteoblasts. In embryonic mouse heads and in cultured tissue explants, we analyzed the expression of selected transcription factors and extracellular matrix molecules associated with bone and cartilage development. Cartilage markers Sox9 and type II collagen were expressed in all craniofacial cartilages. In addition, Msx2 and type I collagen were expressed in sense capsule cartilages. We also observed that the undifferentiated calvarial mesenchyme and the osteogenic fronts in the jaw expressed Col2A1. Moreover, we found that cultured mouse calvarial mesenchyme could develop into cartilage. Of the 49 explants that contained mesenchyme, intramembranous ossification occurred in 35%. Only cartilage formed in 4%, and both cartilage and bone formed in 4%. Our study confirms that calvarial mesenchyme, which normally gives rise to intramembranous bone, also has chondrogenic potential."

    U2 - 10.1369/jhc.4A6518.2005

    DO - 10.1369/jhc.4A6518.2005

    M3 - Article

    VL - 53

    SP - 653

    EP - 663

    JO - Journal of Histochemistry and Cytochemistry

    JF - Journal of Histochemistry and Cytochemistry

    SN - 0022-1554

    IS - 5

    ER -