Stripe rust (Pucinia striformis f.sp. tritici) is one of the most important fungal diseases of wheat, found on all continents and in over 60 countries. Wild emmer wheat, Triticum dicoccoides, which is the tetraploid progenitor of durum wheat, is a valuable source of novel stripe rust resistance genes for wheat breeding. T. dicoccoides G25 accession carries Yr15, a gene on chromosome arm 1BS. Yr15 confers resistance to all known stripe rust isolates; it is also effective in introgressed durum and bread wheat. Retrotransposons generate polymorphic insertions, which can be scored as Mendelian markers with techniques including REMAP and IRAP. Six REMAP and IRAP-derived SCAR markers were developed using 1256 F2 plants derived from crosses of the susceptible T. durum accession D447 with its resistant BC3F9 and BC3F10 (B9 and B10) near isogenic lines, which carried Yr15 introgressed from G25. The nearest markers segregated 0.1 cM proximally and 1.1 cM distally to Yr15. These markers were also mapped and validated at the same position in another independent 500 F2 plants derived from crosses of B9 and B10 with the susceptible cultivar Langdon. SCAR270 and SCAR790, surrounding Yr15 at an interval of 1.2 cM, were found to be reliable and robust co-dominant markers in a wide range of wheat lines and cultivars with and without Yr15. These markers are useful tags in marker-assisted wheat breeding programs aiming to incorporate Yr15 into elite wheat lines and cultivars for durable and broad-spectrum resistance against stripe rust.
- 4111 Jordbruksvetenskap
- 1183 Växtbiologi, mikrobiologi, virologi