Distribution and characterization of endophytic and rhizosphere bacteriome of below-ground tissues in Chinese fir plantation

Kai Wang, Qingao Sang, Liang Hong, Yuxin Liu, Jiyun Yang, Fred Asiegbu, Pengfei Wu, Lin Huang,, Xiangqing Ma

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

Sammanfattning

Plantations of Chinese fir, a popular woody tree species, face sustainable issues, such as nutrient deficiency and increasing disease threat. Rhizosphere and endophytic bacteria play important roles in plants' nutrient absorption and stress alleviation. Our understanding of the microbiome structure and functions is proceeding rapidly in model plants and some crop species. Yet, the spatial distribution and functional patterns of the bacteriome for the woody trees remain largely unexplored. In this study, we collected rhizosphere soil, non-rhizosphere soil, fine root, thick root and primary root samples of Chinese fir and investigated the structure and distribution of bacteriome, as well as the beneficial effects of endophytic bacterial isolates. We discovered that Burkholderia and Paraburkholderia genera were overwhelmingly enriched in rhizosphere soil, and the abundance of Pseudomonas genus was significantly enhanced in fine root. By isolating and testing the nutrient absorption and pathogen antagonism functions of representative endophytic bacteria species in Pseudomonas and Burkholderia, we noticed that phosphorus-solubilizing functional isolates were enriched in fine root, while pathogen antagonism isolates were enriched in thick root. As a conclusion, our study revealed that the endophytic and rhizosphere environments of Chinese fir hold distinct structure and abundance of bacteriomes, with potential specific functional enrichment of some bacterial clades. These findings assist us to further study the potential regulation mechanism of endophytic functional bacteria by the host tree, which will contribute to beneficial microbe application in forestry plantations and sustainable development
Originalspråkengelska
Artikelnummertpae137
TidskriftTree Physiology
Volym44
Nummer11
Antal sidor11
ISSN0829-318X
DOI
StatusPublicerad - 25 nov. 2024
MoE-publikationstypA1 Tidskriftsartikel-refererad

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