Sammanfattning

We studied how plants acclimated to growing conditions that included combinations of blue light (BL) and ultraviolet (UV)-A radiation, and whether their growing environment affected their photosynthetic capacity during and after a brief period of acute high light (as might happen during an under-canopy sunfleck). Arabidopsis thaliana Landsberg erecta wild-type were compared with mutants lacking functional blue light and UV photoreceptors: phototropin 1, cryptochromes (CRY1 and CRY2) and UV RESISTANT LOCUS 8 (uvr8). This was achieved using light-emitting-diode (LED) lamps in a controlled environment to create treatments with or without BL, in a split-plot design with or without UV-A radiation. We compared the accumulation of phenolic compounds under growth conditions and after exposure to 30 min of high light at the end of the experiment (46 days), and likewise measured the operational efficiency of photosystem II (phi PSII, a proxy for photosynthetic performance) and dark-adapted maximum quantum yield (F-v/F-m to assess PSII damage). Our results indicate that cryptochromes are the main photoreceptors regulating phenolic compound accumulation in response to BL and UV-A radiation, and a lack of functional cryptochromes impairs photosynthetic performance under high light. Our findings also reveal a role for UVR8 in accumulating flavonoids in response to a low UV-A dose. Interestingly, phototropin 1 partially mediated constitutive accumulation of phenolic compounds in the absence of BL. Low-irradiance BL and UV-A did not improve phi PSII and F-v/F-m upon our acute high-light treatment; however, CRYs played an important role in ameliorating high-light stress.

Originalspråkengelska
TidskriftPhysiologia Plantarum
Volym165
Utgåva3
Sidor (från-till)537-554
Antal sidor18
ISSN0031-9317
DOI
StatusPublicerad - mar 2019
MoE-publikationstypA1 Tidskriftsartikel-refererad

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  • 1183 Växtbiologi, mikrobiologi, virologi

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@article{6c3e0f5c0d5449549e6d6cae45ca6f96,
title = "Do UV-A radiation and blue light during growth prime leaves to cope with acute high-light in photoreceptor mutants of Arabidopsis thaliana?",
abstract = "We studied how plants acclimated to growing conditions that included combinations of blue light (BL) and ultraviolet (UV)-A radiation, and whether their growing environment affected their photosynthetic capacity during and after a brief period of acute high light (as might happen during an under-canopy sunfleck). Arabidopsis thaliana Landsberg erecta wild-type were compared with mutants lacking functional blue light and UV photoreceptors: phototropin 1, cryptochromes (CRY1 and CRY2) and UV RESISTANT LOCUS 8 (uvr8). This was achieved using light-emitting-diode (LED) lamps in a controlled environment to create treatments with or without BL, in a split-plot design with or without UV-A radiation. We compared the accumulation of phenolic compounds under growth conditions and after exposure to 30 min of high light at the end of the experiment (46 days), and likewise measured the operational efficiency of photosystem II (phi PSII, a proxy for photosynthetic performance) and dark-adapted maximum quantum yield (F-v/F-m to assess PSII damage). Our results indicate that cryptochromes are the main photoreceptors regulating phenolic compound accumulation in response to BL and UV-A radiation, and a lack of functional cryptochromes impairs photosynthetic performance under high light. Our findings also reveal a role for UVR8 in accumulating flavonoids in response to a low UV-A dose. Interestingly, phototropin 1 partially mediated constitutive accumulation of phenolic compounds in the absence of BL. Low-irradiance BL and UV-A did not improve phi PSII and F-v/F-m upon our acute high-light treatment; however, CRYs played an important role in ameliorating high-light stress.",
keywords = "1183 Plant biology, microbiology, virology, SYNTHASE GENE-EXPRESSION, PHOTOSYNTHETICALLY ACTIVE RADIATION, ULTRAVIOLET-B RADIATION, SIGNAL-TRANSDUCTION, PHOTOSYSTEM-II, HIGHER-PLANTS, CHLOROPHYLL FLUORESCENCE, ELECTRON-TRANSPORT, LEAF CHLOROPHYLL, RED-LIGHT",
author = "Craig Brelsford and {Morales Suarez}, {Luis Orlando} and Jakub Nezval and Kotilainen, {Titta Katariina} and Hartikainen, {Saara Maria} and Aphalo, {Pedro J.} and Robson, {Thomas Matthew}",
year = "2019",
month = "3",
doi = "10.1111/ppl.12749",
language = "English",
volume = "165",
pages = "537--554",
journal = "Physiologia Plantarum",
issn = "0031-9317",
publisher = "Wiley",
number = "3",

}

TY - JOUR

T1 - Do UV-A radiation and blue light during growth prime leaves to cope with acute high-light in photoreceptor mutants of Arabidopsis thaliana?

AU - Brelsford, Craig

AU - Morales Suarez, Luis Orlando

AU - Nezval, Jakub

AU - Kotilainen, Titta Katariina

AU - Hartikainen, Saara Maria

AU - Aphalo, Pedro J.

AU - Robson, Thomas Matthew

PY - 2019/3

Y1 - 2019/3

N2 - We studied how plants acclimated to growing conditions that included combinations of blue light (BL) and ultraviolet (UV)-A radiation, and whether their growing environment affected their photosynthetic capacity during and after a brief period of acute high light (as might happen during an under-canopy sunfleck). Arabidopsis thaliana Landsberg erecta wild-type were compared with mutants lacking functional blue light and UV photoreceptors: phototropin 1, cryptochromes (CRY1 and CRY2) and UV RESISTANT LOCUS 8 (uvr8). This was achieved using light-emitting-diode (LED) lamps in a controlled environment to create treatments with or without BL, in a split-plot design with or without UV-A radiation. We compared the accumulation of phenolic compounds under growth conditions and after exposure to 30 min of high light at the end of the experiment (46 days), and likewise measured the operational efficiency of photosystem II (phi PSII, a proxy for photosynthetic performance) and dark-adapted maximum quantum yield (F-v/F-m to assess PSII damage). Our results indicate that cryptochromes are the main photoreceptors regulating phenolic compound accumulation in response to BL and UV-A radiation, and a lack of functional cryptochromes impairs photosynthetic performance under high light. Our findings also reveal a role for UVR8 in accumulating flavonoids in response to a low UV-A dose. Interestingly, phototropin 1 partially mediated constitutive accumulation of phenolic compounds in the absence of BL. Low-irradiance BL and UV-A did not improve phi PSII and F-v/F-m upon our acute high-light treatment; however, CRYs played an important role in ameliorating high-light stress.

AB - We studied how plants acclimated to growing conditions that included combinations of blue light (BL) and ultraviolet (UV)-A radiation, and whether their growing environment affected their photosynthetic capacity during and after a brief period of acute high light (as might happen during an under-canopy sunfleck). Arabidopsis thaliana Landsberg erecta wild-type were compared with mutants lacking functional blue light and UV photoreceptors: phototropin 1, cryptochromes (CRY1 and CRY2) and UV RESISTANT LOCUS 8 (uvr8). This was achieved using light-emitting-diode (LED) lamps in a controlled environment to create treatments with or without BL, in a split-plot design with or without UV-A radiation. We compared the accumulation of phenolic compounds under growth conditions and after exposure to 30 min of high light at the end of the experiment (46 days), and likewise measured the operational efficiency of photosystem II (phi PSII, a proxy for photosynthetic performance) and dark-adapted maximum quantum yield (F-v/F-m to assess PSII damage). Our results indicate that cryptochromes are the main photoreceptors regulating phenolic compound accumulation in response to BL and UV-A radiation, and a lack of functional cryptochromes impairs photosynthetic performance under high light. Our findings also reveal a role for UVR8 in accumulating flavonoids in response to a low UV-A dose. Interestingly, phototropin 1 partially mediated constitutive accumulation of phenolic compounds in the absence of BL. Low-irradiance BL and UV-A did not improve phi PSII and F-v/F-m upon our acute high-light treatment; however, CRYs played an important role in ameliorating high-light stress.

KW - 1183 Plant biology, microbiology, virology

KW - SYNTHASE GENE-EXPRESSION

KW - PHOTOSYNTHETICALLY ACTIVE RADIATION

KW - ULTRAVIOLET-B RADIATION

KW - SIGNAL-TRANSDUCTION

KW - PHOTOSYSTEM-II

KW - HIGHER-PLANTS

KW - CHLOROPHYLL FLUORESCENCE

KW - ELECTRON-TRANSPORT

KW - LEAF CHLOROPHYLL

KW - RED-LIGHT

U2 - 10.1111/ppl.12749

DO - 10.1111/ppl.12749

M3 - Article

VL - 165

SP - 537

EP - 554

JO - Physiologia Plantarum

JF - Physiologia Plantarum

SN - 0031-9317

IS - 3

ER -