Effects of Intra-Articular Botulinum Toxin A on Joint Cartilage and Synovium in the Canine Stifle Joint

Forskningsoutput: KonferensbidragPosterForskningPeer review

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Introduction: Intra-articular botulinum toxin A (IA BoNT A) has produced pain relief in osteoarthritic dogs and human patients without clinical adverse effects. However, its effects on intra-articular tissues have not been studied. Our purpose was to investigate the effects of IA BoNT A on joint cartilage and synovium. The hypothesis was that the toxin would not cause adverse effects on the intra-articular tissues. Material and Methods: Six healthy laboratory beagle dogs assigned for euthanasia due to other reasons were studied in a placebo-controlled, blinded, randomized trial approved by the Committee of Experimental Animals (ESAVI-2010-04178/Ym-23). Dogs were randomized to receive an injection of 30IU BoNT A (Botox®; Allergan Pharmaceuticals Ireland) into the right or left stifle joint. Placebo (0.9% saline) was injected into the contralateral joint. Synovial fluid (SF) was analyzed before and 2,4,8, and 12 weeks after the IA injections. Dogs were euthanized after 12 weeks. The stifle joints were macroscopically evaluated. Biopsies were taken from the articular cartilage and synovium. The histopathological evaluation of the cartilage and synovium biopsies was performed according to the OARSI Histopathology Initiative by two independent, blinded reviewers. The cartilage was evaluated by scoring its structural integrity and chondrocyte pathology. The synovium was evaluated by scoring the number of lining cell layers and the presence of villous hyperplasia and inflammatory cell infiltrates. The obtained scores were used for statistical analysis. Repeated measures ANOVA, logistic regression analysis, and cumulative logit-model were used in the statistical analysis. Statistical significance level was set to P < 0.05. Results: SF cell count was in the reference range in all samples analyzed. There was no statistically significant difference in the SF neutrophil percentage (P = 0.960) between the BoNT A and placebo injected joints during the study. No pathological changes were detected in the macroscopical evaluation of the stifle joints. No statistically significant difference was detected in the scores for cartilage and synovial structure and synovial inflammatory cell infiltrates between the BoNT A and placebo injected joints. Conclusion: Our results suggest that IA BoNT A does not have degenerative or inflammatory effects on joint cartilage or synovium.
Originalspråkengelska
StatusPublicerad - 13 jul 2017
EvenemangECVS Annual Scientific Meeting - Edinburgh, Storbritannien
Varaktighet: 13 jul 201715 jul 2017

Konferens

KonferensECVS Annual Scientific Meeting
LandStorbritannien
OrtEdinburgh
Period13/07/201715/07/2017

Vetenskapsgrenar

  • 413 Veterinärvetenskap

Citera det här

Heikkilä, H. M., Syrjä, P. E. S., Junnila, J., Hielm-Bjorkman, A., & Vapaavuori, O. M. I. (2017). Effects of Intra-Articular Botulinum Toxin A on Joint Cartilage and Synovium in the Canine Stifle Joint. Poster presenterad vid ECVS Annual Scientific Meeting, Edinburgh, Storbritannien.
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title = "Effects of Intra-Articular Botulinum Toxin A on Joint Cartilage and Synovium in the Canine Stifle Joint",
abstract = "Introduction: Intra-articular botulinum toxin A (IA BoNT A) has produced pain relief in osteoarthritic dogs and human patients without clinical adverse effects. However, its effects on intra-articular tissues have not been studied. Our purpose was to investigate the effects of IA BoNT A on joint cartilage and synovium. The hypothesis was that the toxin would not cause adverse effects on the intra-articular tissues. Material and Methods: Six healthy laboratory beagle dogs assigned for euthanasia due to other reasons were studied in a placebo-controlled, blinded, randomized trial approved by the Committee of Experimental Animals (ESAVI-2010-04178/Ym-23). Dogs were randomized to receive an injection of 30IU BoNT A (Botox{\circledR}; Allergan Pharmaceuticals Ireland) into the right or left stifle joint. Placebo (0.9{\%} saline) was injected into the contralateral joint. Synovial fluid (SF) was analyzed before and 2,4,8, and 12 weeks after the IA injections. Dogs were euthanized after 12 weeks. The stifle joints were macroscopically evaluated. Biopsies were taken from the articular cartilage and synovium. The histopathological evaluation of the cartilage and synovium biopsies was performed according to the OARSI Histopathology Initiative by two independent, blinded reviewers. The cartilage was evaluated by scoring its structural integrity and chondrocyte pathology. The synovium was evaluated by scoring the number of lining cell layers and the presence of villous hyperplasia and inflammatory cell infiltrates. The obtained scores were used for statistical analysis. Repeated measures ANOVA, logistic regression analysis, and cumulative logit-model were used in the statistical analysis. Statistical significance level was set to P < 0.05. Results: SF cell count was in the reference range in all samples analyzed. There was no statistically significant difference in the SF neutrophil percentage (P = 0.960) between the BoNT A and placebo injected joints during the study. No pathological changes were detected in the macroscopical evaluation of the stifle joints. No statistically significant difference was detected in the scores for cartilage and synovial structure and synovial inflammatory cell infiltrates between the BoNT A and placebo injected joints. Conclusion: Our results suggest that IA BoNT A does not have degenerative or inflammatory effects on joint cartilage or synovium.",
keywords = "413 Veterinary science",
author = "Heikkil{\"a}, {Helka Maaria} and Syrj{\"a}, {Pernilla Elisabet Sofia} and Jouni Junnila and Anna Hielm-Bjorkman and Vapaavuori, {Outi Maria Inkeri}",
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Heikkilä, HM, Syrjä, PES, Junnila, J, Hielm-Bjorkman, A & Vapaavuori, OMI 2017, 'Effects of Intra-Articular Botulinum Toxin A on Joint Cartilage and Synovium in the Canine Stifle Joint' ECVS Annual Scientific Meeting, Edinburgh, Storbritannien, 13/07/2017 - 15/07/2017, .

Effects of Intra-Articular Botulinum Toxin A on Joint Cartilage and Synovium in the Canine Stifle Joint. / Heikkilä, Helka Maaria; Syrjä, Pernilla Elisabet Sofia; Junnila, Jouni; Hielm-Bjorkman, Anna; Vapaavuori, Outi Maria Inkeri.

2017. Poster presenterad vid ECVS Annual Scientific Meeting, Edinburgh, Storbritannien.

Forskningsoutput: KonferensbidragPosterForskningPeer review

TY - CONF

T1 - Effects of Intra-Articular Botulinum Toxin A on Joint Cartilage and Synovium in the Canine Stifle Joint

AU - Heikkilä, Helka Maaria

AU - Syrjä, Pernilla Elisabet Sofia

AU - Junnila, Jouni

AU - Hielm-Bjorkman, Anna

AU - Vapaavuori, Outi Maria Inkeri

PY - 2017/7/13

Y1 - 2017/7/13

N2 - Introduction: Intra-articular botulinum toxin A (IA BoNT A) has produced pain relief in osteoarthritic dogs and human patients without clinical adverse effects. However, its effects on intra-articular tissues have not been studied. Our purpose was to investigate the effects of IA BoNT A on joint cartilage and synovium. The hypothesis was that the toxin would not cause adverse effects on the intra-articular tissues. Material and Methods: Six healthy laboratory beagle dogs assigned for euthanasia due to other reasons were studied in a placebo-controlled, blinded, randomized trial approved by the Committee of Experimental Animals (ESAVI-2010-04178/Ym-23). Dogs were randomized to receive an injection of 30IU BoNT A (Botox®; Allergan Pharmaceuticals Ireland) into the right or left stifle joint. Placebo (0.9% saline) was injected into the contralateral joint. Synovial fluid (SF) was analyzed before and 2,4,8, and 12 weeks after the IA injections. Dogs were euthanized after 12 weeks. The stifle joints were macroscopically evaluated. Biopsies were taken from the articular cartilage and synovium. The histopathological evaluation of the cartilage and synovium biopsies was performed according to the OARSI Histopathology Initiative by two independent, blinded reviewers. The cartilage was evaluated by scoring its structural integrity and chondrocyte pathology. The synovium was evaluated by scoring the number of lining cell layers and the presence of villous hyperplasia and inflammatory cell infiltrates. The obtained scores were used for statistical analysis. Repeated measures ANOVA, logistic regression analysis, and cumulative logit-model were used in the statistical analysis. Statistical significance level was set to P < 0.05. Results: SF cell count was in the reference range in all samples analyzed. There was no statistically significant difference in the SF neutrophil percentage (P = 0.960) between the BoNT A and placebo injected joints during the study. No pathological changes were detected in the macroscopical evaluation of the stifle joints. No statistically significant difference was detected in the scores for cartilage and synovial structure and synovial inflammatory cell infiltrates between the BoNT A and placebo injected joints. Conclusion: Our results suggest that IA BoNT A does not have degenerative or inflammatory effects on joint cartilage or synovium.

AB - Introduction: Intra-articular botulinum toxin A (IA BoNT A) has produced pain relief in osteoarthritic dogs and human patients without clinical adverse effects. However, its effects on intra-articular tissues have not been studied. Our purpose was to investigate the effects of IA BoNT A on joint cartilage and synovium. The hypothesis was that the toxin would not cause adverse effects on the intra-articular tissues. Material and Methods: Six healthy laboratory beagle dogs assigned for euthanasia due to other reasons were studied in a placebo-controlled, blinded, randomized trial approved by the Committee of Experimental Animals (ESAVI-2010-04178/Ym-23). Dogs were randomized to receive an injection of 30IU BoNT A (Botox®; Allergan Pharmaceuticals Ireland) into the right or left stifle joint. Placebo (0.9% saline) was injected into the contralateral joint. Synovial fluid (SF) was analyzed before and 2,4,8, and 12 weeks after the IA injections. Dogs were euthanized after 12 weeks. The stifle joints were macroscopically evaluated. Biopsies were taken from the articular cartilage and synovium. The histopathological evaluation of the cartilage and synovium biopsies was performed according to the OARSI Histopathology Initiative by two independent, blinded reviewers. The cartilage was evaluated by scoring its structural integrity and chondrocyte pathology. The synovium was evaluated by scoring the number of lining cell layers and the presence of villous hyperplasia and inflammatory cell infiltrates. The obtained scores were used for statistical analysis. Repeated measures ANOVA, logistic regression analysis, and cumulative logit-model were used in the statistical analysis. Statistical significance level was set to P < 0.05. Results: SF cell count was in the reference range in all samples analyzed. There was no statistically significant difference in the SF neutrophil percentage (P = 0.960) between the BoNT A and placebo injected joints during the study. No pathological changes were detected in the macroscopical evaluation of the stifle joints. No statistically significant difference was detected in the scores for cartilage and synovial structure and synovial inflammatory cell infiltrates between the BoNT A and placebo injected joints. Conclusion: Our results suggest that IA BoNT A does not have degenerative or inflammatory effects on joint cartilage or synovium.

KW - 413 Veterinary science

M3 - Poster

ER -

Heikkilä HM, Syrjä PES, Junnila J, Hielm-Bjorkman A, Vapaavuori OMI. Effects of Intra-Articular Botulinum Toxin A on Joint Cartilage and Synovium in the Canine Stifle Joint. 2017. Poster presenterad vid ECVS Annual Scientific Meeting, Edinburgh, Storbritannien.