Establishing a community-wide DNA barcode library as a new tool for arctic research

Helena Kristiina Wirta, Gergely Várkonyi, Claus Rasmussen, Riikka Maria Kaartinen, N. M. Schmidt, P. D. N. Hebert, M Barták, G. Blagoev, H. Disney, S. Ertl, P. Gjelstrup, D. J. Gwiazdowicz, L. Huldén, J. Ilmonen, J. Jakovlev, M. Jaschhof, Jere Veikko Kahanpää, Tuomas Tapio Kankaanpää, P. H. Krogh, C. Labbee & 12 andra C. Lettner, V. Michelsen, S. A. Nielsen, T. R. Nielsen, L. Paasivirta, S. Pedersen, J. Pohjoismäki, Jukka Antti Matias Salmela, Pekka Antero Vilkamaa, Henry Uolevi Väre, M. von Tschirnhaus, Tomas Valter Roslin

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

Sammanfattning

DNA sequences offer powerful tools for describing the members and interactions of natural communities. In this study, we establish the to-date most comprehensive library of DNA barcodes for a terrestrial site, including all known macroscopic animals and vascular plants of an intensively studied area of the High Arctic, the Zackenberg Valley in Northeast Greenland. To demonstrate its utility, we apply the library to identify nearly 20 000 arthropod individuals from two Malaise traps, each operated for two summers. Drawing on this material, we estimate the coverage of previous morphology-based species inventories, derive a snapshot of faunal turnover in space and time and describe the abundance and phenology of species in the rapidly changing arctic environment. Overall, 403 terrestrial animal and 160 vascular plant species were recorded by morphology-based techniques. DNA barcodes (CO1) offered high resolution in discriminating among the local animal taxa, with 92% of morphologically distinguishable taxa assigned to unique Barcode Index Numbers (BINs) and 93% to monophyletic clusters. For vascular plants, resolution was lower, with 54% of species forming monophyletic clusters based on barcode regions rbcLa and ITS2. Malaise catches revealed 122 BINs not detected by previous sampling and DNA barcoding. The insect community was dominated by a few highly abundant taxa. Even closely related taxa differed in phenology, emphasizing the need for species-level resolution when describing ongoing shifts in arctic communities and ecosystems. The DNA barcode library now established for Zackenberg offers new scope for such explorations, and for the detailed dissection of interspecific interactions throughout the community.
Originalspråkengelska
TidskriftMolecular Ecology Resources
Volym16
Utgåva3
Sidor (från-till)809-822
Antal sidor14
ISSN1755-098X
DOI
StatusPublicerad - 2016
MoE-publikationstypA1 Tidskriftsartikel-refererad

Vetenskapsgrenar

  • 1181 Ekologi, evolutionsbiologi
  • 1182 Biokemi, cell- och molekylärbiologi

Citera det här

Wirta, Helena Kristiina ; Várkonyi, Gergely ; Rasmussen, Claus ; Kaartinen, Riikka Maria ; Schmidt, N. M. ; Hebert, P. D. N. ; Barták, M ; Blagoev, G. ; Disney, H. ; Ertl, S. ; Gjelstrup, P. ; Gwiazdowicz, D. J. ; Huldén, L. ; Ilmonen, J. ; Jakovlev, J. ; Jaschhof, M. ; Kahanpää, Jere Veikko ; Kankaanpää, Tuomas Tapio ; Krogh, P. H. ; Labbee, C. ; Lettner, C. ; Michelsen, V. ; Nielsen, S. A. ; Nielsen, T. R. ; Paasivirta, L. ; Pedersen, S. ; Pohjoismäki, J. ; Salmela, Jukka Antti Matias ; Vilkamaa, Pekka Antero ; Väre, Henry Uolevi ; von Tschirnhaus, M. ; Roslin, Tomas Valter. / Establishing a community-wide DNA barcode library as a new tool for arctic research. I: Molecular Ecology Resources. 2016 ; Vol. 16, Nr. 3. s. 809-822.
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title = "Establishing a community-wide DNA barcode library as a new tool for arctic research",
abstract = "DNA sequences offer powerful tools for describing the members and interactions of natural communities. In this study, we establish the to-date most comprehensive library of DNA barcodes for a terrestrial site, including all known macroscopic animals and vascular plants of an intensively studied area of the High Arctic, the Zackenberg Valley in Northeast Greenland. To demonstrate its utility, we apply the library to identify nearly 20 000 arthropod individuals from two Malaise traps, each operated for two summers. Drawing on this material, we estimate the coverage of previous morphology-based species inventories, derive a snapshot of faunal turnover in space and time and describe the abundance and phenology of species in the rapidly changing arctic environment. Overall, 403 terrestrial animal and 160 vascular plant species were recorded by morphology-based techniques. DNA barcodes (CO1) offered high resolution in discriminating among the local animal taxa, with 92{\%} of morphologically distinguishable taxa assigned to unique Barcode Index Numbers (BINs) and 93{\%} to monophyletic clusters. For vascular plants, resolution was lower, with 54{\%} of species forming monophyletic clusters based on barcode regions rbcLa and ITS2. Malaise catches revealed 122 BINs not detected by previous sampling and DNA barcoding. The insect community was dominated by a few highly abundant taxa. Even closely related taxa differed in phenology, emphasizing the need for species-level resolution when describing ongoing shifts in arctic communities and ecosystems. The DNA barcode library now established for Zackenberg offers new scope for such explorations, and for the detailed dissection of interspecific interactions throughout the community.",
keywords = "1181 Ecology, evolutionary biology, 1182 Biochemistry, cell and molecular biology",
author = "Wirta, {Helena Kristiina} and Gergely V{\'a}rkonyi and Claus Rasmussen and Kaartinen, {Riikka Maria} and Schmidt, {N. M.} and Hebert, {P. D. N.} and M Bart{\'a}k and G. Blagoev and H. Disney and S. Ertl and P. Gjelstrup and Gwiazdowicz, {D. J.} and L. Huld{\'e}n and J. Ilmonen and J. Jakovlev and M. Jaschhof and Kahanp{\"a}{\"a}, {Jere Veikko} and Kankaanp{\"a}{\"a}, {Tuomas Tapio} and Krogh, {P. H.} and C. Labbee and C. Lettner and V. Michelsen and Nielsen, {S. A.} and Nielsen, {T. R.} and L. Paasivirta and S. Pedersen and J. Pohjoism{\"a}ki and Salmela, {Jukka Antti Matias} and Vilkamaa, {Pekka Antero} and V{\"a}re, {Henry Uolevi} and {von Tschirnhaus}, M. and Roslin, {Tomas Valter}",
year = "2016",
doi = "10.1111/1755-0998.12489",
language = "English",
volume = "16",
pages = "809--822",
journal = "Molecular Ecology Resources",
issn = "1755-098X",
publisher = "Wiley",
number = "3",

}

Wirta, HK, Várkonyi, G, Rasmussen, C, Kaartinen, RM, Schmidt, NM, Hebert, PDN, Barták, M, Blagoev, G, Disney, H, Ertl, S, Gjelstrup, P, Gwiazdowicz, DJ, Huldén, L, Ilmonen, J, Jakovlev, J, Jaschhof, M, Kahanpää, JV, Kankaanpää, TT, Krogh, PH, Labbee, C, Lettner, C, Michelsen, V, Nielsen, SA, Nielsen, TR, Paasivirta, L, Pedersen, S, Pohjoismäki, J, Salmela, JAM, Vilkamaa, PA, Väre, HU, von Tschirnhaus, M & Roslin, TV 2016, 'Establishing a community-wide DNA barcode library as a new tool for arctic research', Molecular Ecology Resources, vol. 16, nr. 3, s. 809-822. https://doi.org/10.1111/1755-0998.12489

Establishing a community-wide DNA barcode library as a new tool for arctic research. / Wirta, Helena Kristiina; Várkonyi, Gergely; Rasmussen, Claus; Kaartinen, Riikka Maria; Schmidt, N. M.; Hebert, P. D. N.; Barták, M; Blagoev, G.; Disney, H.; Ertl, S.; Gjelstrup, P.; Gwiazdowicz, D. J.; Huldén, L.; Ilmonen, J.; Jakovlev, J.; Jaschhof, M.; Kahanpää, Jere Veikko; Kankaanpää, Tuomas Tapio; Krogh, P. H.; Labbee, C.; Lettner, C.; Michelsen, V.; Nielsen, S. A.; Nielsen, T. R.; Paasivirta, L.; Pedersen, S.; Pohjoismäki, J.; Salmela, Jukka Antti Matias; Vilkamaa, Pekka Antero; Väre, Henry Uolevi; von Tschirnhaus, M.; Roslin, Tomas Valter.

I: Molecular Ecology Resources, Vol. 16, Nr. 3, 2016, s. 809-822.

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

TY - JOUR

T1 - Establishing a community-wide DNA barcode library as a new tool for arctic research

AU - Wirta, Helena Kristiina

AU - Várkonyi, Gergely

AU - Rasmussen, Claus

AU - Kaartinen, Riikka Maria

AU - Schmidt, N. M.

AU - Hebert, P. D. N.

AU - Barták, M

AU - Blagoev, G.

AU - Disney, H.

AU - Ertl, S.

AU - Gjelstrup, P.

AU - Gwiazdowicz, D. J.

AU - Huldén, L.

AU - Ilmonen, J.

AU - Jakovlev, J.

AU - Jaschhof, M.

AU - Kahanpää, Jere Veikko

AU - Kankaanpää, Tuomas Tapio

AU - Krogh, P. H.

AU - Labbee, C.

AU - Lettner, C.

AU - Michelsen, V.

AU - Nielsen, S. A.

AU - Nielsen, T. R.

AU - Paasivirta, L.

AU - Pedersen, S.

AU - Pohjoismäki, J.

AU - Salmela, Jukka Antti Matias

AU - Vilkamaa, Pekka Antero

AU - Väre, Henry Uolevi

AU - von Tschirnhaus, M.

AU - Roslin, Tomas Valter

PY - 2016

Y1 - 2016

N2 - DNA sequences offer powerful tools for describing the members and interactions of natural communities. In this study, we establish the to-date most comprehensive library of DNA barcodes for a terrestrial site, including all known macroscopic animals and vascular plants of an intensively studied area of the High Arctic, the Zackenberg Valley in Northeast Greenland. To demonstrate its utility, we apply the library to identify nearly 20 000 arthropod individuals from two Malaise traps, each operated for two summers. Drawing on this material, we estimate the coverage of previous morphology-based species inventories, derive a snapshot of faunal turnover in space and time and describe the abundance and phenology of species in the rapidly changing arctic environment. Overall, 403 terrestrial animal and 160 vascular plant species were recorded by morphology-based techniques. DNA barcodes (CO1) offered high resolution in discriminating among the local animal taxa, with 92% of morphologically distinguishable taxa assigned to unique Barcode Index Numbers (BINs) and 93% to monophyletic clusters. For vascular plants, resolution was lower, with 54% of species forming monophyletic clusters based on barcode regions rbcLa and ITS2. Malaise catches revealed 122 BINs not detected by previous sampling and DNA barcoding. The insect community was dominated by a few highly abundant taxa. Even closely related taxa differed in phenology, emphasizing the need for species-level resolution when describing ongoing shifts in arctic communities and ecosystems. The DNA barcode library now established for Zackenberg offers new scope for such explorations, and for the detailed dissection of interspecific interactions throughout the community.

AB - DNA sequences offer powerful tools for describing the members and interactions of natural communities. In this study, we establish the to-date most comprehensive library of DNA barcodes for a terrestrial site, including all known macroscopic animals and vascular plants of an intensively studied area of the High Arctic, the Zackenberg Valley in Northeast Greenland. To demonstrate its utility, we apply the library to identify nearly 20 000 arthropod individuals from two Malaise traps, each operated for two summers. Drawing on this material, we estimate the coverage of previous morphology-based species inventories, derive a snapshot of faunal turnover in space and time and describe the abundance and phenology of species in the rapidly changing arctic environment. Overall, 403 terrestrial animal and 160 vascular plant species were recorded by morphology-based techniques. DNA barcodes (CO1) offered high resolution in discriminating among the local animal taxa, with 92% of morphologically distinguishable taxa assigned to unique Barcode Index Numbers (BINs) and 93% to monophyletic clusters. For vascular plants, resolution was lower, with 54% of species forming monophyletic clusters based on barcode regions rbcLa and ITS2. Malaise catches revealed 122 BINs not detected by previous sampling and DNA barcoding. The insect community was dominated by a few highly abundant taxa. Even closely related taxa differed in phenology, emphasizing the need for species-level resolution when describing ongoing shifts in arctic communities and ecosystems. The DNA barcode library now established for Zackenberg offers new scope for such explorations, and for the detailed dissection of interspecific interactions throughout the community.

KW - 1181 Ecology, evolutionary biology

KW - 1182 Biochemistry, cell and molecular biology

U2 - 10.1111/1755-0998.12489

DO - 10.1111/1755-0998.12489

M3 - Article

VL - 16

SP - 809

EP - 822

JO - Molecular Ecology Resources

JF - Molecular Ecology Resources

SN - 1755-098X

IS - 3

ER -