Establishment of a reverse genetics system for studying human bocavirus in human airway epithelia

Qinfeng Huang, Xuefeng Deng, Ziying Yan, Fang Cheng, Yong Luo, Weiran Shen, Diana C.M. Lei-Butters, Aaron Yun Chen, Yi Li, Liang Tang, Maria Söderlund-Venermo, John F Engelhardt, Jianming Qiu

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Sammanfattning

Human bocavirus 1 (HBoV1) has been identified as one of the etiological agents of wheezing in young children with acute
respiratory-tract infections. In this study, we have obtained the sequence of a full-length HBoV1 genome (including both
termini) using viral DNA extracted from a nasopharyngeal aspirate of an infected patient, cloned the full-length HBoV1
genome, and demonstrated DNA replication, encapsidation of the ssDNA genome, and release of the HBoV1 virions from
human embryonic kidney 293 cells. The HBoV1 virions generated from this cell line-based production system exhibits a
typical icosahedral structure of approximately 26 nm in diameter, and is capable of productively infecting polarized primary
human airway epithelia (HAE) from the apical surface. Infected HAE showed hallmarks of lung airway-tract injury, including
disruption of the tight junction barrier, loss of cilia and epithelial cell hypertrophy. Notably, polarized HAE cultured from an
immortalized airway epithelial cell line, CuFi-8 (originally derived from a cystic fibrosis patient), also supported productive
infection of HBoV1. Thus, we have established a reverse genetics system and generated the first cell line-based culture
system for the study of HBoV1 infection, which will significantly advance the study of HBoV1
Originalspråkengelska
TidskriftPLoS Pathogens
Volym8
Utgåva8
Sidor (från-till)e1002899
Antal sidor14
ISSN1553-7366
DOI
StatusPublicerad - 2012
MoE-publikationstypA1 Tidskriftsartikel-refererad

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