Evaluation of a diagnostic microarray for the detection of major bacterial pathogens of potato from tuber samples

Yeshitila Degefu, Panu Juhani Somervuo, Marja Aittamaa, Elina Virtanen, Jari Pekka Tapani Valkonen

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

Sammanfattning

Potato can be infected with many bacterial pathogens, the detection of which is necessary in seed certification. In this study, a diagnostic microarray previously tested for specificity of probes for detecting the potato bacteria causing blackleg and soft rot (Pectobacterium atrosepticum, Pectobacterium carotovorum, and Dickeya spp.), ring rot (Clavibacter michiganensis subsp. sepedonicus), scab (Streptomyces scabies and Streptomyces turgidiscabies) and brown rot (Ralstonia solanacearum) from pure culture was evaluated for analytical sensitivity when testing directly from tuber samples. The microarray readily detected all the bacterial species when 100 ng of the target bacterial DNA from pure culture was mixed with DNA from soil microbes and potato. However, detection was inconsistent when total DNA isolated directly from infected tubers or enriched bacterial culture was used. While the high specificity of the probes could be confirmed from the results of the DNA cocktail experiment used as a control, the study demonstrated that the level of analytical sensitivity of the microarray under the tested condition was not sufficient to detect bacteria directly from tubers. Therefore, in addition to the cost and organizational complexities, the low analytical sensitivity and limited reproducibility of the microarray are constraints for establishing the platform for routine detection of potato bacterial pathogens from tuber samples.
Originalspråkengelska
TidskriftEPPO Bulletin
Volym46
Utgåva1
Sidor (från-till)103-111
Antal sidor9
ISSN0250-8052
DOI
StatusPublicerad - 8 mar 2016
MoE-publikationstypA1 Tidskriftsartikel-refererad

Vetenskapsgrenar

  • 4111 Jordbruksvetenskap

Citera det här