Extracellular vesicles derived from hypoxic colorectal cancer cells confer metastatic phenotype to non-metastatic cancer cells

Edgars Endzelins, Arturs Abols, Arturs Buss, Elina Zandberga, Mari Johanna Palviainen, Pia Riitta-Maria Siljander, Aija Line

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

Sammanfattning

Background/Aim: Tumor-secreted extracellular
vesicles (EVs) play an important role as mediators of
intercellular communication. Hypoxia is a common feature
of solid tumors frequently associated with an aggressive
clinical behavior. This study aimed to gain a deeper
understanding into the functions of EVs in intercellular
communication between primary and metastatic cancer cells
under hypoxic conditions. Materials and Methods: EVs were
isolated from two isogenic colorectal cancer (CRC) cell lines
SW480 and SW620 cultured under normoxic and hypoxic
conditions. Their uptake and effects in SW480 and SW620
cells were studied using EV uptake, proliferation, spheroidformation,
wound healing and invasion assays. Results: Our
data showed that hypoxia enhanced the release of EVs from
CRC cells in a Hypoxia Induced Factor (HIF)-1-dependent
manner. Hypoxic EVs were taken up by CRC cells more
efficiently than normoxic EVs. Hypoxic EVs stimulated
motility, invasiveness and stemness of primary tumourderived
SW480 cells, whereas they had a little effect on
metastasis-derived SW620 cells. Conclusion: Hypoxic
colorectal cancer-derived EVs confer aggressiveness and
invasiveness to hypoxia-naïve cancer cells.
Originalspråkengelska
TidskriftAnticancer Research
Volym38
Utgåva9
Sidor (från-till)5169-5147
Antal sidor9
ISSN0250-7005
DOI
StatusPublicerad - 28 aug 2018
MoE-publikationstypA1 Tidskriftsartikel-refererad

Vetenskapsgrenar

  • 317 Farmaci

Citera det här

@article{2ee7835cd234424baa58f8f1c2dd30cc,
title = "Extracellular vesicles derived from hypoxic colorectal cancer cells confer metastatic phenotype to non-metastatic cancer cells",
abstract = "Background/Aim: Tumor-secreted extracellularvesicles (EVs) play an important role as mediators ofintercellular communication. Hypoxia is a common featureof solid tumors frequently associated with an aggressiveclinical behavior. This study aimed to gain a deeperunderstanding into the functions of EVs in intercellularcommunication between primary and metastatic cancer cellsunder hypoxic conditions. Materials and Methods: EVs wereisolated from two isogenic colorectal cancer (CRC) cell linesSW480 and SW620 cultured under normoxic and hypoxicconditions. Their uptake and effects in SW480 and SW620cells were studied using EV uptake, proliferation, spheroidformation,wound healing and invasion assays. Results: Ourdata showed that hypoxia enhanced the release of EVs fromCRC cells in a Hypoxia Induced Factor (HIF)-1-dependentmanner. Hypoxic EVs were taken up by CRC cells moreefficiently than normoxic EVs. Hypoxic EVs stimulatedmotility, invasiveness and stemness of primary tumourderivedSW480 cells, whereas they had a little effect onmetastasis-derived SW620 cells. Conclusion: Hypoxiccolorectal cancer-derived EVs confer aggressiveness andinvasiveness to hypoxia-na{\"i}ve cancer cells.",
keywords = "317 Pharmacy, Extracellular vesicles, colorectal cancer, hypoxia, PROMOTE ANGIOGENESIS, CARCINOMA-CELLS, EXOSOMES, EXPRESSION, STEMNESS",
author = "Edgars Endzelins and Arturs Abols and Arturs Buss and Elina Zandberga and Palviainen, {Mari Johanna} and Siljander, {Pia Riitta-Maria} and Aija Line",
year = "2018",
month = "8",
day = "28",
doi = "10.21873/anticanres.12836",
language = "English",
volume = "38",
pages = "5169--5147",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "INTERNATIONAL INSTITUTE OF ANTICANCER RESEARCH",
number = "9",

}

Extracellular vesicles derived from hypoxic colorectal cancer cells confer metastatic phenotype to non-metastatic cancer cells. / Endzelins, Edgars; Abols, Arturs; Buss, Arturs ; Zandberga, Elina ; Palviainen, Mari Johanna; Siljander, Pia Riitta-Maria; Line, Aija.

I: Anticancer Research, Vol. 38, Nr. 9, 28.08.2018, s. 5169-5147.

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

TY - JOUR

T1 - Extracellular vesicles derived from hypoxic colorectal cancer cells confer metastatic phenotype to non-metastatic cancer cells

AU - Endzelins, Edgars

AU - Abols, Arturs

AU - Buss, Arturs

AU - Zandberga, Elina

AU - Palviainen, Mari Johanna

AU - Siljander, Pia Riitta-Maria

AU - Line, Aija

PY - 2018/8/28

Y1 - 2018/8/28

N2 - Background/Aim: Tumor-secreted extracellularvesicles (EVs) play an important role as mediators ofintercellular communication. Hypoxia is a common featureof solid tumors frequently associated with an aggressiveclinical behavior. This study aimed to gain a deeperunderstanding into the functions of EVs in intercellularcommunication between primary and metastatic cancer cellsunder hypoxic conditions. Materials and Methods: EVs wereisolated from two isogenic colorectal cancer (CRC) cell linesSW480 and SW620 cultured under normoxic and hypoxicconditions. Their uptake and effects in SW480 and SW620cells were studied using EV uptake, proliferation, spheroidformation,wound healing and invasion assays. Results: Ourdata showed that hypoxia enhanced the release of EVs fromCRC cells in a Hypoxia Induced Factor (HIF)-1-dependentmanner. Hypoxic EVs were taken up by CRC cells moreefficiently than normoxic EVs. Hypoxic EVs stimulatedmotility, invasiveness and stemness of primary tumourderivedSW480 cells, whereas they had a little effect onmetastasis-derived SW620 cells. Conclusion: Hypoxiccolorectal cancer-derived EVs confer aggressiveness andinvasiveness to hypoxia-naïve cancer cells.

AB - Background/Aim: Tumor-secreted extracellularvesicles (EVs) play an important role as mediators ofintercellular communication. Hypoxia is a common featureof solid tumors frequently associated with an aggressiveclinical behavior. This study aimed to gain a deeperunderstanding into the functions of EVs in intercellularcommunication between primary and metastatic cancer cellsunder hypoxic conditions. Materials and Methods: EVs wereisolated from two isogenic colorectal cancer (CRC) cell linesSW480 and SW620 cultured under normoxic and hypoxicconditions. Their uptake and effects in SW480 and SW620cells were studied using EV uptake, proliferation, spheroidformation,wound healing and invasion assays. Results: Ourdata showed that hypoxia enhanced the release of EVs fromCRC cells in a Hypoxia Induced Factor (HIF)-1-dependentmanner. Hypoxic EVs were taken up by CRC cells moreefficiently than normoxic EVs. Hypoxic EVs stimulatedmotility, invasiveness and stemness of primary tumourderivedSW480 cells, whereas they had a little effect onmetastasis-derived SW620 cells. Conclusion: Hypoxiccolorectal cancer-derived EVs confer aggressiveness andinvasiveness to hypoxia-naïve cancer cells.

KW - 317 Pharmacy

KW - Extracellular vesicles

KW - colorectal cancer

KW - hypoxia

KW - PROMOTE ANGIOGENESIS

KW - CARCINOMA-CELLS

KW - EXOSOMES

KW - EXPRESSION

KW - STEMNESS

U2 - 10.21873/anticanres.12836

DO - 10.21873/anticanres.12836

M3 - Article

VL - 38

SP - 5169

EP - 5147

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 9

ER -