F4 (K88) fimbrial adhesin FaeG expressed in alfalfa reduces F4+ enterotoxigenic Escherichia coli excretion in weaned piglets

J. J Joensuu, F Verdonck, Andrea Ehrström, M Peltola, H Siljander-Rasi, A. M Nuutila, K.-M Oksman-Caldentey, T. H Teeri, E Cox, B. M Goddeeris, V Niklander-Teeri, Jussi Joensuu, Teemu Teeri, Viola Niklander-Teeri

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

Sammanfattning

Transgenic plants are attractive bioreactors to large-scale production of recombinant proteins because of their relatively low cost. This study reports for the first time the use of transgenic plants to reduce enterotoxigenic Escherichia coli (ETEC) excretion in its natural host species. The DNA sequence encoding the major subunit and adhesin FaeG of F4+ ETEC was transformed into edible alfalfa plants. Targeting of FaeG production to chloroplasts led to FaeG levels of up to 1% of the total soluble protein fraction of the transgenic alfalfa. Recombinant plant-produced FaeG (pFaeG) remained stable for 2 years when the plant material was dried and stored at room temperature. Intragastric immunization of piglets with pFaeG induced a weak F4-specific humoral response. Co-administration of pFaeG and the mucosal adjuvant cholera toxin (CT) enhanced the immune response against FaeG, reflected a better induction of an F4-specifie immune response. In addition, the intragastric co-administration of CT with pFaeG significantly reduced F4+ E. coli excretion following F4+ ETEC challenge as compared with pigs that had received nontransgenic plant material. In conclusion, transgenic plants producing the FaeG subunit protein could be used for production and delivery of oral vaccines against F4+ ETEC infections. (c) 2005 Elsevier Ltd. All rights reserved.
Originalspråkengelska
TidskriftVaccine
Volym24
Sidor (från-till)2387-2394
Antal sidor8
ISSN0264-410X
DOI
StatusPublicerad - 2006
MoE-publikationstypA1 Tidskriftsartikel-refererad

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  • 412 Djursvetenskap, mjölkproduktlära
  • 414 Jordbruksbioteknologi
  • 411 Jordbruks- och skogsvetenskaper

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