Heterologous Expression of the Leuconostoc Bacteriocin Leucocin C in Probiotic Yeast Saccharomyces boulardii

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Sammanfattning

The yeastSaccharomyces boulardiiis well known for its probiotic effects such as treating or preventing gastrointestinal diseases. Due to its ability to survive in stomach and intestine,S. boulardiicould be applied as a vehicle for producing and delivering bioactive substances of interest to human gut. In this study, we cloned the genelecCencoding the antilisterial peptide leucocin C from lactic acid bacteriumLeuconostoc carnosuminS. boulardii. The constructedS. boulardiistrain secreted a peptide, which had molecular weight corresponding to leucocin C in SDS-PAGE. The peptide band inhibitedListeria monocytogenesin gel overlay assay. Likewise, concentratedS. boulardiiculture supernatant inhibited the growth ofL. monocytogenes. The growth profile and acid tolerance of the leucocin C secretingS. boulardiiwere similar as those of the strain carrying the empty vector. We further demonstrated that the cells of the leucocin C producingS. boulardiiefficiently killedL. monocytogenes, also without antibiotic selection pressure. These results showed that antilisterial activity could be added to the arsenal of probiotic activities ofS. boulardii, demonstrating its potential as a carrier for therapeutics delivery.

Originalspråkengelska
TidskriftProbiotics and Antimicrobial Proteins
Antal sidor9
ISSN1867-1306
DOI
Status!!E-pub ahead of print - 21 jun 2020
MoE-publikationstypA1 Tidskriftsartikel-refererad

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  • 416 Livsmedelsvetenskap
  • 11832 Mikrobiologi och virologi

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