High genetic relatedness among Mycobacterium avium strains isolated from pigs and humans revealed by comparative IS1245 RFLP analysis

Taneli Tirkkonen, Jaakko Pakarinen, Anna-Maria Moisander, J Mäkinen, H Soini, Terhi Ali-Vehmas

    Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

    Sammanfattning

    "Members of the Mycobacterium avium complex cause pig mycobacteriosis and opportunistic human infections. Infections due to environmental mycobacteria are increasing in both industrial and developing countries. Mycobacterium-infected pig carcasses can pass for human consumption due to the poor specificity of meat control by visual detection at the slaughter houses. The genetic relatedness of porcine and human MAC isolates in Finland has been unknown. M. avium isolates isolated from pig organs (n = 16) and clinical samples (n = 13) were compared by IS/245 RFLP analysis to evaluate the similarity of the isolates obtained from human and porcine samples. Nearly identical multicopy M. avium subsp. hominissuis IS 1245 RFLP fingerprints were obtained for isolates of porcine and human origin. IS/245 RFLP patterns of 38% of the porcine and human M. a. hominissuis isolates were >90% similar. The RFLP patterns of two porcine and two human isolates showed >95% similarity. The high similarity of the IS1245 RFLP patterns of the human and porcine M. a. hominissuis isolates indicates close genetic relatedness, suggesting that M. a. hominissuis is transmitted between pigs and humans, or that pigs and humans share common environmental sources of infection. Porcine and human isolates with RFLP patterns differing by only one or two bands were found, which shows that the same M. a. hominissuis strains may infect both humans and pigs. (C) 2007 Elsevier B.V. All rights reserved."
    Originalspråkengelska
    TidskriftVeterinary Microbiology
    Volym125
    Utgåva1-2
    Sidor (från-till)175-181
    Antal sidor7
    ISSN0378-1135
    DOI
    StatusPublicerad - 2007
    MoE-publikationstypA1 Tidskriftsartikel-refererad

    Vetenskapsgrenar

    • 413 Veterinärvetenskap
    • bakteerit
    • Mycobacterium avium
    • ihminen
    • sika
    • zoonoosit
    • elintarviketurvallisuus
    • molekyyliepidemiologia

    Citera det här

    Tirkkonen, Taneli ; Pakarinen, Jaakko ; Moisander, Anna-Maria ; Mäkinen, J ; Soini, H ; Ali-Vehmas, Terhi. / High genetic relatedness among Mycobacterium avium strains isolated from pigs and humans revealed by comparative IS1245 RFLP analysis. I: Veterinary Microbiology. 2007 ; Vol. 125, Nr. 1-2. s. 175-181.
    @article{4b7960e50c4844baaad9a67baf87467e,
    title = "High genetic relatedness among Mycobacterium avium strains isolated from pigs and humans revealed by comparative IS1245 RFLP analysis",
    abstract = "{"}Members of the Mycobacterium avium complex cause pig mycobacteriosis and opportunistic human infections. Infections due to environmental mycobacteria are increasing in both industrial and developing countries. Mycobacterium-infected pig carcasses can pass for human consumption due to the poor specificity of meat control by visual detection at the slaughter houses. The genetic relatedness of porcine and human MAC isolates in Finland has been unknown. M. avium isolates isolated from pig organs (n = 16) and clinical samples (n = 13) were compared by IS/245 RFLP analysis to evaluate the similarity of the isolates obtained from human and porcine samples. Nearly identical multicopy M. avium subsp. hominissuis IS 1245 RFLP fingerprints were obtained for isolates of porcine and human origin. IS/245 RFLP patterns of 38{\%} of the porcine and human M. a. hominissuis isolates were >90{\%} similar. The RFLP patterns of two porcine and two human isolates showed >95{\%} similarity. The high similarity of the IS1245 RFLP patterns of the human and porcine M. a. hominissuis isolates indicates close genetic relatedness, suggesting that M. a. hominissuis is transmitted between pigs and humans, or that pigs and humans share common environmental sources of infection. Porcine and human isolates with RFLP patterns differing by only one or two bands were found, which shows that the same M. a. hominissuis strains may infect both humans and pigs. (C) 2007 Elsevier B.V. All rights reserved.{"}",
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    author = "Taneli Tirkkonen and Jaakko Pakarinen and Anna-Maria Moisander and J M{\"a}kinen and H Soini and Terhi Ali-Vehmas",
    year = "2007",
    doi = "10.1016/j.vetmic.2007.05.005",
    language = "English",
    volume = "125",
    pages = "175--181",
    journal = "Veterinary Microbiology",
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    High genetic relatedness among Mycobacterium avium strains isolated from pigs and humans revealed by comparative IS1245 RFLP analysis. / Tirkkonen, Taneli; Pakarinen, Jaakko; Moisander, Anna-Maria; Mäkinen, J; Soini, H; Ali-Vehmas, Terhi.

    I: Veterinary Microbiology, Vol. 125, Nr. 1-2, 2007, s. 175-181.

    Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

    TY - JOUR

    T1 - High genetic relatedness among Mycobacterium avium strains isolated from pigs and humans revealed by comparative IS1245 RFLP analysis

    AU - Tirkkonen, Taneli

    AU - Pakarinen, Jaakko

    AU - Moisander, Anna-Maria

    AU - Mäkinen, J

    AU - Soini, H

    AU - Ali-Vehmas, Terhi

    PY - 2007

    Y1 - 2007

    N2 - "Members of the Mycobacterium avium complex cause pig mycobacteriosis and opportunistic human infections. Infections due to environmental mycobacteria are increasing in both industrial and developing countries. Mycobacterium-infected pig carcasses can pass for human consumption due to the poor specificity of meat control by visual detection at the slaughter houses. The genetic relatedness of porcine and human MAC isolates in Finland has been unknown. M. avium isolates isolated from pig organs (n = 16) and clinical samples (n = 13) were compared by IS/245 RFLP analysis to evaluate the similarity of the isolates obtained from human and porcine samples. Nearly identical multicopy M. avium subsp. hominissuis IS 1245 RFLP fingerprints were obtained for isolates of porcine and human origin. IS/245 RFLP patterns of 38% of the porcine and human M. a. hominissuis isolates were >90% similar. The RFLP patterns of two porcine and two human isolates showed >95% similarity. The high similarity of the IS1245 RFLP patterns of the human and porcine M. a. hominissuis isolates indicates close genetic relatedness, suggesting that M. a. hominissuis is transmitted between pigs and humans, or that pigs and humans share common environmental sources of infection. Porcine and human isolates with RFLP patterns differing by only one or two bands were found, which shows that the same M. a. hominissuis strains may infect both humans and pigs. (C) 2007 Elsevier B.V. All rights reserved."

    AB - "Members of the Mycobacterium avium complex cause pig mycobacteriosis and opportunistic human infections. Infections due to environmental mycobacteria are increasing in both industrial and developing countries. Mycobacterium-infected pig carcasses can pass for human consumption due to the poor specificity of meat control by visual detection at the slaughter houses. The genetic relatedness of porcine and human MAC isolates in Finland has been unknown. M. avium isolates isolated from pig organs (n = 16) and clinical samples (n = 13) were compared by IS/245 RFLP analysis to evaluate the similarity of the isolates obtained from human and porcine samples. Nearly identical multicopy M. avium subsp. hominissuis IS 1245 RFLP fingerprints were obtained for isolates of porcine and human origin. IS/245 RFLP patterns of 38% of the porcine and human M. a. hominissuis isolates were >90% similar. The RFLP patterns of two porcine and two human isolates showed >95% similarity. The high similarity of the IS1245 RFLP patterns of the human and porcine M. a. hominissuis isolates indicates close genetic relatedness, suggesting that M. a. hominissuis is transmitted between pigs and humans, or that pigs and humans share common environmental sources of infection. Porcine and human isolates with RFLP patterns differing by only one or two bands were found, which shows that the same M. a. hominissuis strains may infect both humans and pigs. (C) 2007 Elsevier B.V. All rights reserved."

    KW - 413 Veterinary science

    KW - bakteerit

    KW - Mycobacterium avium

    KW - ihminen

    KW - sika

    KW - zoonoosit

    KW - elintarviketurvallisuus

    KW - molekyyliepidemiologia

    KW - bakteerit

    KW - Mycobacterium avium

    KW - ihminen

    KW - sika

    KW - zoonoosit

    KW - elintarviketurvallisuus

    KW - molekyyliepidemiologia

    KW - bakteerit

    KW - Mycobacterium avium

    KW - ihminen

    KW - sika

    KW - zoonoosit

    KW - elintarviketurvallisuus

    KW - molekyyliepidemiologia

    U2 - 10.1016/j.vetmic.2007.05.005

    DO - 10.1016/j.vetmic.2007.05.005

    M3 - Article

    VL - 125

    SP - 175

    EP - 181

    JO - Veterinary Microbiology

    JF - Veterinary Microbiology

    SN - 0378-1135

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    ER -