Human serum paraoxonase (PON1) activity down regulates with age: A case study in south Indian population

Prasad A. J. Mahadesh, Bala Chauhan Jyoti, B. M. Kavyashree , Kumar N. Vijith, Arun Anjali, Komal Kumar Javarappa, Rao S. R. Guruprasada

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review


With immense understanding on the role of Human serum Paraoxonase 1 (PON1) as a cardio protective molecule and its anti-atherogenic property, there are significant amount of research is going on evaluating the level of PON1 in different geographical region to understand the risk of cardiovascular diseases. The present study was conducted to analyze PON1 activity in 110 Individuals age group ranging from 18-87 years of both sex from south Indians. The PON1 activity was measured as aryl esterase activity using the substrate phenyl acetate. Additionally, we have also partially purified human serum PON1 using cibacron blue affinity matrix to assess the PON1 association with its natural environment. The statistical analysis of PON1 activity from analyzed samples revealed that PON1 activity was negatively correlated with age. Further, the partial purification results also showed that PON1 enzyme was dissociated from HDL natural microenvironment and this explains possible reason for the decline in PON1 activity with increasing age.
TidskriftAsian Journal of Biochemical and Pharmaceutical Research
Sidor (från-till)170-176
Antal sidor7
StatusPublicerad - 1 jun 2016
Externt publiceradJa
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