Investigating Mitophagy and Mitochondrial Morphology In Vivo Using mito-QC: A Comprehensive Guide

Thomas McWilliams, Ian G. Ganley

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Sammanfattning

Autophagy evolved as a mechanism to sustain cellular homeostasis during instances of nutrient deprivation. Mounting evidence has also clarified that under basal and stress conditions, selective autophagy pathways can target the destruction of specific organelles. Mitochondrial autophagy, or mitophagy, has emerged as a key quality control (QC) mechanism to sustain the integrity of eukaryotic mitochondrial networks. We recently reported the development of mito-QC, a novel reporter mouse model that enables the high-resolution study of mammalian mitophagy with precision, in fixed and live preparations. This model holds significant potential to transform our understanding of mammalian mitophagy pathways in vivo, in a variety of physiological contexts. We outline a detailed protocol for use of our recently described mito-QC mouse model, including tips and troubleshooting advice for those interested in monitoring mitophagy in vitro and in vivo.
Originalspråkengelska
Titel på gästpublikationAutophagy : Methods and Protocols
RedaktörerNicholas Ktistakis, Oliver Florey
Antal sidor22
FörlagSpringer Nature
Utgivningsdatum5 jan 2019
Utgåva1st ed.
Sidor621-642
ISBN (tryckt)978-1-4939-8872-3
ISBN (elektroniskt)978-1-4939-8873-0
DOI
StatusPublicerad - 5 jan 2019
MoE-publikationstypA3 Del av bok eller annan forskningsbok

Publikationsserier

NamnMethods in Molecular Biology
FörlagHumana Press, Inc.
Nummer1880
ISSN (tryckt)1064-3745
ISSN (elektroniskt)1940-6029

Vetenskapsgrenar

  • 3112 Neurovetenskaper
  • 1182 Biokemi, cell- och molekylärbiologi
  • 3111 Biomedicinska vetenskaper
  • 1184 Genetik, utvecklingsbiologi, fysiologi

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