Isolation, characterization, and potential application of Acinetobacter baumannii phages against extensively drug-resistant strains

Sanaz Rastegar, Mikael Skurnik, Hira Niaz, Omid Tadjrobehkar, Ali Samareh, Hossein Hosseini-Nave, Salehe Sabouri

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

Sammanfattning

One of the significant issues in treating bacterial infections is the increasing prevalence of extensively drug-resistant (XDR) strains of Acinetobacter baumannii. In the face of limited or no viable treatment options for extensively drug-resistant (XDR) bacteria, there is a renewed interest in utilizing bacteriophages as a treatment option. Three Acinetobacter phages (vB_AbaS_Ftm, vB_AbaS_Eva, and vB_AbaS_Gln) were identified from hospital sewage and analyzed for their morphology, host ranges, and their genome sequences were determined and annotated. These phages and vB_AbaS_SA1 were combined to form a phage cocktail. The antibacterial effects of this cocktail and its combinations with selected antimicrobial agents were evaluated against the XDR A. baumannii strains. The phages exhibited siphovirus morphology. Out of a total of 30 XDR A. baumannii isolates, 33% were sensitive to vB_AbaS_Ftm, 30% to vB_AbaS_Gln, and 16.66% to vB_AbaS_Eva. When these phages were combined with antibiotics, they demonstrated a synergistic effect. The genome sizes of vB_AbaS_Ftm, vB_AbaS_Eva, and vB_AbaS_Gln were 48487, 50174, and 50043 base pairs (bp), respectively, and showed high similarity. Phage cocktail, when combined with antibiotics, showed synergistic effects on extensively drug-resistant (XDR) strains of A. baumannii. However, the need for further study to fully understand the mechanisms of action and potential limitations of using these phages is highlighted.

Originalspråkengelska
TidskriftVirus Genes
Antal sidor12
ISSN0920-8569
DOI
StatusPublicerad - 2024
MoE-publikationstypA1 Tidskriftsartikel-refererad

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© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024.

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  • 3111 Biomedicinska vetenskaper
  • 11832 Mikrobiologi och virologi

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