Label-free imaging of adipogenesis by coherent anti-stokes Raman scattering microscopy

A. Isomäki, T. Sillat, M. Ainola, M. Liljestrom, Y. T. Konttinen, M. Hukkanen

Forskningsoutput: Kapitel i bok/rapport/konferenshandlingKapitelVetenskaplig

Sammanfattning

Label-free imaging technologies to monitor the events associated with
early, intermediate and late adipogenic differentiation in multipotent
mesenchymal stromal cells (MSCs) offer an attractive and convenient
alternative to conventional fixative based lipid dyes such as Oil Red O
and Sudan Red, fluorescent labels such as LipidTOX, and more indirect
methods such as qRT-PCR analyses of specific adipocyte differentiation
markers such as peroxisome PPARγ and LPL. Coherent anti-Stokes
Raman scattering (CARS) microscopy of live cells is a sensitive and fast
imaging method enabling evaluation of the adipogenic differentiation
with chemical specificity. CARS microscopy is based on imaging
structures of interest by displaying the characteristic intrinsic vibrational
contrast of chemical bonds. The method is nontoxic, non-destructive, and
minimally invasive, thus presenting a promising method for longitudinal
analyses of live cells and tissues. CARS provides a coherently emitted
signal that is much stronger than the spontaneous Raman scattering.
The anti-Stokes signal is blue shifted from the incident wavelength,
thus reducing the non-vibrational background present in most biological
materials. In this chapter, we aim to provide a detailed approach on how
to induce adipogenic differentiation in MSC cultures, and present our
methods related to label-free CARS imaging of the events associated
with the adipogenesis.
Originalspråkengelska
Titel på gästpublikationArthritis Research
Antal sidor13
Volym1142
FörlagHumana Press
Utgivningsdatum2014
Sidor189-201
ISBN (tryckt)1064-3745
DOI
StatusPublicerad - 2014
MoE-publikationstypB2 Del av bok eller annan forskningsbok

Publikationsserier

NamnMethods in Molecular Biology
Nummer1142
Volym2014
ISSN (tryckt)1064-3745
ISSN (elektroniskt)1940-6029

Vetenskapsgrenar

  • 3111 Biomedicinska vetenskaper

Citera det här

Isomäki, A., Sillat, T., Ainola, M., Liljestrom, M., Konttinen, Y. T., & Hukkanen, M. (2014). Label-free imaging of adipogenesis by coherent anti-stokes Raman scattering microscopy. I Arthritis Research (Vol. 1142, s. 189-201). (Methods in Molecular Biology; Vol. 2014, Nr. 1142). Humana Press. https://doi.org/10.1007/978-1-4939-0404-4_16 [doi]
Isomäki, A. ; Sillat, T. ; Ainola, M. ; Liljestrom, M. ; Konttinen, Y. T. ; Hukkanen, M. / Label-free imaging of adipogenesis by coherent anti-stokes Raman scattering microscopy. Arthritis Research. Vol. 1142 Humana Press, 2014. s. 189-201 (Methods in Molecular Biology; 1142).
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abstract = "Label-free imaging technologies to monitor the events associated withearly, intermediate and late adipogenic differentiation in multipotentmesenchymal stromal cells (MSCs) offer an attractive and convenientalternative to conventional fixative based lipid dyes such as Oil Red Oand Sudan Red, fluorescent labels such as LipidTOX, and more indirectmethods such as qRT-PCR analyses of specific adipocyte differentiationmarkers such as peroxisome PPARγ and LPL. Coherent anti-StokesRaman scattering (CARS) microscopy of live cells is a sensitive and fastimaging method enabling evaluation of the adipogenic differentiationwith chemical specificity. CARS microscopy is based on imagingstructures of interest by displaying the characteristic intrinsic vibrationalcontrast of chemical bonds. The method is nontoxic, non-destructive, andminimally invasive, thus presenting a promising method for longitudinalanalyses of live cells and tissues. CARS provides a coherently emittedsignal that is much stronger than the spontaneous Raman scattering.The anti-Stokes signal is blue shifted from the incident wavelength,thus reducing the non-vibrational background present in most biologicalmaterials. In this chapter, we aim to provide a detailed approach on howto induce adipogenic differentiation in MSC cultures, and present ourmethods related to label-free CARS imaging of the events associatedwith the adipogenesis.",
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Isomäki, A, Sillat, T, Ainola, M, Liljestrom, M, Konttinen, YT & Hukkanen, M 2014, Label-free imaging of adipogenesis by coherent anti-stokes Raman scattering microscopy. i Arthritis Research. vol. 1142, Methods in Molecular Biology, nr. 1142, vol. 2014, Humana Press, s. 189-201. https://doi.org/10.1007/978-1-4939-0404-4_16 [doi]

Label-free imaging of adipogenesis by coherent anti-stokes Raman scattering microscopy. / Isomäki, A.; Sillat, T.; Ainola, M.; Liljestrom, M.; Konttinen, Y. T.; Hukkanen, M.

Arthritis Research. Vol. 1142 Humana Press, 2014. s. 189-201 (Methods in Molecular Biology; Vol. 2014, Nr. 1142).

Forskningsoutput: Kapitel i bok/rapport/konferenshandlingKapitelVetenskaplig

TY - CHAP

T1 - Label-free imaging of adipogenesis by coherent anti-stokes Raman scattering microscopy

AU - Isomäki, A.

AU - Sillat, T.

AU - Ainola, M.

AU - Liljestrom, M.

AU - Konttinen, Y. T.

AU - Hukkanen, M.

PY - 2014

Y1 - 2014

N2 - Label-free imaging technologies to monitor the events associated withearly, intermediate and late adipogenic differentiation in multipotentmesenchymal stromal cells (MSCs) offer an attractive and convenientalternative to conventional fixative based lipid dyes such as Oil Red Oand Sudan Red, fluorescent labels such as LipidTOX, and more indirectmethods such as qRT-PCR analyses of specific adipocyte differentiationmarkers such as peroxisome PPARγ and LPL. Coherent anti-StokesRaman scattering (CARS) microscopy of live cells is a sensitive and fastimaging method enabling evaluation of the adipogenic differentiationwith chemical specificity. CARS microscopy is based on imagingstructures of interest by displaying the characteristic intrinsic vibrationalcontrast of chemical bonds. The method is nontoxic, non-destructive, andminimally invasive, thus presenting a promising method for longitudinalanalyses of live cells and tissues. CARS provides a coherently emittedsignal that is much stronger than the spontaneous Raman scattering.The anti-Stokes signal is blue shifted from the incident wavelength,thus reducing the non-vibrational background present in most biologicalmaterials. In this chapter, we aim to provide a detailed approach on howto induce adipogenic differentiation in MSC cultures, and present ourmethods related to label-free CARS imaging of the events associatedwith the adipogenesis.

AB - Label-free imaging technologies to monitor the events associated withearly, intermediate and late adipogenic differentiation in multipotentmesenchymal stromal cells (MSCs) offer an attractive and convenientalternative to conventional fixative based lipid dyes such as Oil Red Oand Sudan Red, fluorescent labels such as LipidTOX, and more indirectmethods such as qRT-PCR analyses of specific adipocyte differentiationmarkers such as peroxisome PPARγ and LPL. Coherent anti-StokesRaman scattering (CARS) microscopy of live cells is a sensitive and fastimaging method enabling evaluation of the adipogenic differentiationwith chemical specificity. CARS microscopy is based on imagingstructures of interest by displaying the characteristic intrinsic vibrationalcontrast of chemical bonds. The method is nontoxic, non-destructive, andminimally invasive, thus presenting a promising method for longitudinalanalyses of live cells and tissues. CARS provides a coherently emittedsignal that is much stronger than the spontaneous Raman scattering.The anti-Stokes signal is blue shifted from the incident wavelength,thus reducing the non-vibrational background present in most biologicalmaterials. In this chapter, we aim to provide a detailed approach on howto induce adipogenic differentiation in MSC cultures, and present ourmethods related to label-free CARS imaging of the events associatedwith the adipogenesis.

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VL - 1142

T3 - Methods in Molecular Biology

SP - 189

EP - 201

BT - Arthritis Research

PB - Humana Press

ER -

Isomäki A, Sillat T, Ainola M, Liljestrom M, Konttinen YT, Hukkanen M. Label-free imaging of adipogenesis by coherent anti-stokes Raman scattering microscopy. I Arthritis Research. Vol. 1142. Humana Press. 2014. s. 189-201. (Methods in Molecular Biology; 1142). https://doi.org/10.1007/978-1-4939-0404-4_16 [doi]