Dextran-producing Weissella have received significant attention. However, except for maltose, the acceptor reactions of Weissella dextransucrases with different sugars have not been investigated. The action of recombinant Weissella confuse VIT E-90392 dextransucrase was tested with several potential acceptors, particularly, analogs lactose and cellobiose. The major acceptor products of both disaccharides were identified as branched trisaccharides, with a glucosyl residue alpha-(1 -> 2)-linked to the acceptor's reducing end. An additional product, isomelezitose (6(Fru)-alpha-Glcp-sucrose), was also produced when using lactose as an acceptor. This is the first report of the synthesis of isomelezitose by a dextransucrase. The NMR spectra of the three trisaccharides were fully assigned, and their structures were confirmed by selective enzymatic hydrolysis. The trisaccharides prepared from C-13(6)glc sucrose and lactose were analyzed by ESI-MSn, and the fragmentation patterns of these compounds were characterized. (C) 2015 Elsevier Ltd. All rights reserved.
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Shi, Q., Juvonen, M. K., Hou, Y., Kajala, I., Nyyssölä, A., Maina, H. N., ... Tenkanen, T. M. (2016). Lactose- and cellobiose-derived branched trisaccharides and a sucrose-containing trisaccharide produced by acceptor reactions of Weissella confusa dextransucrase. Food Chemistry, 109(January), 226-236. https://doi.org/10.1016/j.foodchem.2015.05.090