Sammanfattning
Ljungan virus (LV) is a rodent-borne virus in the genus Parechovirus of the Picornaviridae family. LV has been detected in rodents in Northern Europe, North America and Italy, suggesting a wide distribution of this virus in the world. LV antibodies have been demonstrated in humans with myocarditis and type 1 diabetes, and LV has been correlated with intrauterine death, sudden infant death and fetal central nervous system malformations in humans.
This study aimed to obtain information about the presence of LV, which has not been investigated in Turkey, in wild rodents in Zonguldak province and to determine the new LV strain(s) specific to the region, if any.
A total of 120 brain tissues from 85 Apodemus and 35 Myodes were used in the study. In these samples, the presence of LV nucleic acid was investigated by reverse transcriptase polymerase chain reaction (PCR) method targeting the VP1 region in the LV genome where genetic diversity is high. As a result of the study, the presence of LV nucleic acid was not detected in the samples.
It will not be a sufficient explanation for the absence of LV presence in Turkey according to the analysis of samples from a single region and two rodent species. Unsuitable environmental conditions for the virus may also have played a role in the lack of virus detection. We concluded that further research on rodents in different regions, with a larger sample size and on more rodent species, is needed to reveal the occurrence of LV in Turkey. This is the first study on Ljungan virus presence in Turkey.
This study aimed to obtain information about the presence of LV, which has not been investigated in Turkey, in wild rodents in Zonguldak province and to determine the new LV strain(s) specific to the region, if any.
A total of 120 brain tissues from 85 Apodemus and 35 Myodes were used in the study. In these samples, the presence of LV nucleic acid was investigated by reverse transcriptase polymerase chain reaction (PCR) method targeting the VP1 region in the LV genome where genetic diversity is high. As a result of the study, the presence of LV nucleic acid was not detected in the samples.
It will not be a sufficient explanation for the absence of LV presence in Turkey according to the analysis of samples from a single region and two rodent species. Unsuitable environmental conditions for the virus may also have played a role in the lack of virus detection. We concluded that further research on rodents in different regions, with a larger sample size and on more rodent species, is needed to reveal the occurrence of LV in Turkey. This is the first study on Ljungan virus presence in Turkey.
| Bidragets översatta titel | Investigation of Ljungan virus (Parechovirus B) in rodents by molecular methods |
|---|---|
| Originalspråk | turkiska |
| Tidskrift | ANKEM dergisi |
| Volym | 37 |
| Nummer | 3 |
| Sidor (från-till) | 68-73 |
| Antal sidor | 6 |
| ISSN | 1301-3114 |
| DOI | |
| Status | Publicerad - 31 dec. 2023 |
| MoE-publikationstyp | A1 Tidskriftsartikel-refererad |
Vetenskapsgrenar
- 3111 Biomedicinska vetenskaper