Manipulation of developmental function in turtles with notes on alligators

Jacqueline Moustakas-Verho, Rebecca McLennan, Jennifer Spengler, Paul Kulesa, Judith A. Cebra-Thomas

Forskningsoutput: Kapitel i bok/rapport/konferenshandlingKapitelVetenskaplig

Sammanfattning

Reptiles have great taxonomic diversity that is reflected in their morphology, ecology, physiology, modes of reproduction, and development. Interest in comparative and evolutionary developmental biology makes protocols for the study of reptile embryos invaluable resources. The relatively large size, seasonal breeding, and long gestation times of turtles epitomize the challenges faced by the developmental biologist. We describe protocols for the preparation of turtle embryos for ex ovo culture, electroporation, in situ hybridization, and micro-computed tomography. Because these protocols have been adapted and optimized from methods used for frog, chick, and mouse embryos, it is likely that they could be used for other reptilian species. Notes are included for alligator embryos where appropriate.
Originalspråkengelska
Titel på gästpublikationVertebrate Embryogenesis : Embryological, Cellular and Genetic Methods
Antal sidor17
FörlagSpringer
Utgivningsdatumfeb 2019
Sidor247-263
ISBN (tryckt)978-1-61779-209-0
ISBN (elektroniskt)978-1-61779-210-6
DOI
StatusPublicerad - feb 2019
MoE-publikationstypB2 Del av bok eller annan forskningsbok

Publikationsserier

NamnMethods in Molecular Biology
FörlagHumana Press
ISSN (elektroniskt)1064-3745

Vetenskapsgrenar

  • 1182 Biokemi, cell- och molekylärbiologi

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