Miniaturized whole-cell bacterial bioreporter assay for identification of quorum sensing interfering compounds

Polina Ilina, Xiaochu Ma, Herman O. Sintim, Päivi Sirpa Marjaana Tammela

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

Sammanfattning

The continuing emergence and spread of antibiotic-resistant bacteria is worrisome and new strategies to curb bacterial infections are being sought. The interference of bacterial quorum sensing (QS) signaling has been suggested as a prospective antivirulence strategy. The AI-2 QS system is present in multiple bacterial species and has been shown to be correlated with pathogenicity. To facilitate the discovery of novel compounds interfering with AI-2 QS, we established a high-throughput setup of whole-cell bioreporter assay, which can be performed in either 96- or 384-well format. Agonistic or antagonistic activities of the test compounds against Escherichia coli LsrB-type AI-2 QS system are monitored by measuring the level of beta-galactosidase expression. A control strain expressing beta-galactosidase in quorum sensing-independent manner is included into the assay for false-positive detection.

Originalspråkengelska
TidskriftJournal of Microbiological Methods
Volym154
Sidor (från-till)40-45
Antal sidor6
ISSN0167-7012
DOI
StatusPublicerad - nov 2018
MoE-publikationstypA1 Tidskriftsartikel-refererad

Vetenskapsgrenar

  • 1182 Biokemi, cell- och molekylärbiologi
  • 1183 Växtbiologi, mikrobiologi, virologi
  • 317 Farmaci

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