Miniaturized whole-cell bacterial bioreporter assay for identification of quorum sensing interfering compounds

Polina Ilina; Xiaochu Ma; Herman O. Sintim; Päivi Sirpa Marjaana Tammela

doi:10.1016/j.mimet.2018.10.005

Miniaturized whole-cell bacterial bioreporter assay for identification of quorum sensing interfering compounds

Polina Ilina, Xiaochu Ma, Herman O. Sintim, Päivi Sirpa Marjaana Tammela

Forskningsoutput: Tidskriftsbidrag › Artikel › Vetenskaplig › Peer review

Sammanfattning

The continuing emergence and spread of antibiotic-resistant bacteria is worrisome and new strategies to curb bacterial infections are being sought. The interference of bacterial quorum sensing (QS) signaling has been suggested as a prospective antivirulence strategy. The AI-2 QS system is present in multiple bacterial species and has been shown to be correlated with pathogenicity. To facilitate the discovery of novel compounds interfering with AI-2 QS, we established a high-throughput setup of whole-cell bioreporter assay, which can be performed in either 96- or 384-well format. Agonistic or antagonistic activities of the test compounds against Escherichia coli LsrB-type AI-2 QS system are monitored by measuring the level of beta-galactosidase expression. A control strain expressing beta-galactosidase in quorum sensing-independent manner is included into the assay for false-positive detection.

Originalspråk	engelska
Tidskrift	Journal of Microbiological Methods
Volym	154
Sidor (från-till)	40-45
Antal sidor	6
ISSN	0167-7012
DOI	https://doi.org/10.1016/j.mimet.2018.10.005
Status	Publicerad - nov. 2018
MoE-publikationstyp	A1 Tidskriftsartikel-refererad

Vetenskapsgrenar

1182 Biokemi, cell- och molekylärbiologi
1183 Växtbiologi, mikrobiologi, virologi
317 Farmaci

Åtkomst av dokument

10.1016/j.mimet.2018.10.005Licens: CC BY

1-s2.0-S016770121830736X-mainSlutlig publicerad version, 776 KBLicens: CC BY

Citera det här

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title = "Miniaturized whole-cell bacterial bioreporter assay for identification of quorum sensing interfering compounds",

abstract = "The continuing emergence and spread of antibiotic-resistant bacteria is worrisome and new strategies to curb bacterial infections are being sought. The interference of bacterial quorum sensing (QS) signaling has been suggested as a prospective antivirulence strategy. The AI-2 QS system is present in multiple bacterial species and has been shown to be correlated with pathogenicity. To facilitate the discovery of novel compounds interfering with AI-2 QS, we established a high-throughput setup of whole-cell bioreporter assay, which can be performed in either 96- or 384-well format. Agonistic or antagonistic activities of the test compounds against Escherichia coli LsrB-type AI-2 QS system are monitored by measuring the level of beta-galactosidase expression. A control strain expressing beta-galactosidase in quorum sensing-independent manner is included into the assay for false-positive detection.",

keywords = "AI-2, ART, AUTOINDUCER-2, Assay miniaturization, Autoinducer-2, Bioreporter strain, High throughput screening, INHIBITORS, PERSPECTIVES, Quorum sensing inhibitors, 1182 Biochemistry, cell and molecular biology, 1183 Plant biology, microbiology, virology, 317 Pharmacy",

author = "Polina Ilina and Xiaochu Ma and Sintim, {Herman O.} and Tammela, {P{\"a}ivi Sirpa Marjaana}",

year = "2018",

month = nov,

doi = "10.1016/j.mimet.2018.10.005",

language = "English",

volume = "154",

pages = "40--45",

journal = "Journal of Microbiological Methods",

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TY - JOUR

T1 - Miniaturized whole-cell bacterial bioreporter assay for identification of quorum sensing interfering compounds

AU - Ilina, Polina

AU - Ma, Xiaochu

AU - Sintim, Herman O.

AU - Tammela, Päivi Sirpa Marjaana

PY - 2018/11

Y1 - 2018/11

N2 - The continuing emergence and spread of antibiotic-resistant bacteria is worrisome and new strategies to curb bacterial infections are being sought. The interference of bacterial quorum sensing (QS) signaling has been suggested as a prospective antivirulence strategy. The AI-2 QS system is present in multiple bacterial species and has been shown to be correlated with pathogenicity. To facilitate the discovery of novel compounds interfering with AI-2 QS, we established a high-throughput setup of whole-cell bioreporter assay, which can be performed in either 96- or 384-well format. Agonistic or antagonistic activities of the test compounds against Escherichia coli LsrB-type AI-2 QS system are monitored by measuring the level of beta-galactosidase expression. A control strain expressing beta-galactosidase in quorum sensing-independent manner is included into the assay for false-positive detection.

AB - The continuing emergence and spread of antibiotic-resistant bacteria is worrisome and new strategies to curb bacterial infections are being sought. The interference of bacterial quorum sensing (QS) signaling has been suggested as a prospective antivirulence strategy. The AI-2 QS system is present in multiple bacterial species and has been shown to be correlated with pathogenicity. To facilitate the discovery of novel compounds interfering with AI-2 QS, we established a high-throughput setup of whole-cell bioreporter assay, which can be performed in either 96- or 384-well format. Agonistic or antagonistic activities of the test compounds against Escherichia coli LsrB-type AI-2 QS system are monitored by measuring the level of beta-galactosidase expression. A control strain expressing beta-galactosidase in quorum sensing-independent manner is included into the assay for false-positive detection.

KW - AI-2

KW - ART

KW - AUTOINDUCER-2

KW - Assay miniaturization

KW - Autoinducer-2

KW - Bioreporter strain

KW - High throughput screening

KW - INHIBITORS

KW - PERSPECTIVES

KW - Quorum sensing inhibitors

KW - 1182 Biochemistry, cell and molecular biology

KW - 1183 Plant biology, microbiology, virology

KW - 317 Pharmacy

U2 - 10.1016/j.mimet.2018.10.005

DO - 10.1016/j.mimet.2018.10.005

M3 - Article

SN - 0167-7012

VL - 154

SP - 40

EP - 45

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

ER -