Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens

Paul Daly, Mao Peng, Sara Casado López, Anna Lipzen, Vivian Ng, Vasanth Singan, Mei Wang, Igor Grigoriev, Ronald de Vries, Miia Mäkelä

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

Sammanfattning

Heterologous production of fungal ligninolytic cocktails is challenging due to the low yields of catalytically active lignin modifying peroxidases. Production using a natural system, such as a wood-rotting fungus, is a promising alternative if specific or preferential induction of the ligninolytic activities could be achieved. Using transcriptomics, gene expression of the white-rot Dichomitus squalens during growth on mixtures of aromatic compounds, with ring structures representing the two major lignin sub-units, was compared to a wood substrate. Most of the genes encoding lignin modifying enzymes (laccases and peroxidases) categorised as highly or moderately expressed on wood were expressed similarly on aromatic compounds. Higher expression levels of a subset of manganese and versatile peroxidases was observed on di- compared to mono-methoxylated aromatics. The expression of polysaccharide degrading enzymes was lower on aromatic compounds compared to wood, demonstrating that the induction of lignin modifying enzymes became more specific. This study suggests potential for aromatic waste streams, e.g. from lignocellulose pretreatment, to produce a lignin-specific enzyme cocktail from D. squalens or other white-rot fungi.

Originalspråkengelska
TidskriftJournal of Biotechnology
Volym308
Sidor (från-till)35-39
Antal sidor5
ISSN0168-1656
DOI
StatusPublicerad - 20 jan 2020
MoE-publikationstypA1 Tidskriftsartikel-refererad

Vetenskapsgrenar

  • 1183 Växtbiologi, mikrobiologi, virologi

Citera det här

Daly, Paul ; Peng, Mao ; Casado López, Sara ; Lipzen, Anna ; Ng, Vivian ; Singan, Vasanth ; Wang, Mei ; Grigoriev, Igor ; de Vries, Ronald ; Mäkelä, Miia . / Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens. I: Journal of Biotechnology. 2020 ; Vol. 308. s. 35-39.
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title = "Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens",
abstract = "Heterologous production of fungal ligninolytic cocktails is challenging due to the low yields of catalytically active lignin modifying peroxidases. Production using a natural system, such as a wood-rotting fungus, is a promising alternative if specific or preferential induction of the ligninolytic activities could be achieved. Using transcriptomics, gene expression of the white-rot Dichomitus squalens during growth on mixtures of aromatic compounds, with ring structures representing the two major lignin sub-units, was compared to a wood substrate. Most of the genes encoding lignin modifying enzymes (laccases and peroxidases) categorised as highly or moderately expressed on wood were expressed similarly on aromatic compounds. Higher expression levels of a subset of manganese and versatile peroxidases was observed on di- compared to mono-methoxylated aromatics. The expression of polysaccharide degrading enzymes was lower on aromatic compounds compared to wood, demonstrating that the induction of lignin modifying enzymes became more specific. This study suggests potential for aromatic waste streams, e.g. from lignocellulose pretreatment, to produce a lignin-specific enzyme cocktail from D. squalens or other white-rot fungi.",
keywords = "Aromatics, Basidiomycete, DEGRADATION, Gene expression, LACCASE, Lignin, MANGANESE PEROXIDASE, White-rot, 1183 Plant biology, microbiology, virology",
author = "Paul Daly and Mao Peng and {Casado L{\'o}pez}, Sara and Anna Lipzen and Vivian Ng and Vasanth Singan and Mei Wang and Igor Grigoriev and {de Vries}, Ronald and Miia M{\"a}kel{\"a}",
year = "2020",
month = "1",
day = "20",
doi = "10.1016/j.jbiotec.2019.11.014",
language = "English",
volume = "308",
pages = "35--39",
journal = "Journal of Biotechnology",
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Daly, P, Peng, M, Casado López, S, Lipzen, A, Ng, V, Singan, V, Wang, M, Grigoriev, I, de Vries, R & Mäkelä, M 2020, 'Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens', Journal of Biotechnology, vol. 308, s. 35-39. https://doi.org/10.1016/j.jbiotec.2019.11.014

Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens. / Daly, Paul; Peng, Mao; Casado López, Sara; Lipzen, Anna; Ng, Vivian; Singan, Vasanth; Wang, Mei ; Grigoriev, Igor; de Vries, Ronald; Mäkelä, Miia .

I: Journal of Biotechnology, Vol. 308, 20.01.2020, s. 35-39.

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

TY - JOUR

T1 - Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens

AU - Daly, Paul

AU - Peng, Mao

AU - Casado López, Sara

AU - Lipzen, Anna

AU - Ng, Vivian

AU - Singan, Vasanth

AU - Wang, Mei

AU - Grigoriev, Igor

AU - de Vries, Ronald

AU - Mäkelä, Miia

PY - 2020/1/20

Y1 - 2020/1/20

N2 - Heterologous production of fungal ligninolytic cocktails is challenging due to the low yields of catalytically active lignin modifying peroxidases. Production using a natural system, such as a wood-rotting fungus, is a promising alternative if specific or preferential induction of the ligninolytic activities could be achieved. Using transcriptomics, gene expression of the white-rot Dichomitus squalens during growth on mixtures of aromatic compounds, with ring structures representing the two major lignin sub-units, was compared to a wood substrate. Most of the genes encoding lignin modifying enzymes (laccases and peroxidases) categorised as highly or moderately expressed on wood were expressed similarly on aromatic compounds. Higher expression levels of a subset of manganese and versatile peroxidases was observed on di- compared to mono-methoxylated aromatics. The expression of polysaccharide degrading enzymes was lower on aromatic compounds compared to wood, demonstrating that the induction of lignin modifying enzymes became more specific. This study suggests potential for aromatic waste streams, e.g. from lignocellulose pretreatment, to produce a lignin-specific enzyme cocktail from D. squalens or other white-rot fungi.

AB - Heterologous production of fungal ligninolytic cocktails is challenging due to the low yields of catalytically active lignin modifying peroxidases. Production using a natural system, such as a wood-rotting fungus, is a promising alternative if specific or preferential induction of the ligninolytic activities could be achieved. Using transcriptomics, gene expression of the white-rot Dichomitus squalens during growth on mixtures of aromatic compounds, with ring structures representing the two major lignin sub-units, was compared to a wood substrate. Most of the genes encoding lignin modifying enzymes (laccases and peroxidases) categorised as highly or moderately expressed on wood were expressed similarly on aromatic compounds. Higher expression levels of a subset of manganese and versatile peroxidases was observed on di- compared to mono-methoxylated aromatics. The expression of polysaccharide degrading enzymes was lower on aromatic compounds compared to wood, demonstrating that the induction of lignin modifying enzymes became more specific. This study suggests potential for aromatic waste streams, e.g. from lignocellulose pretreatment, to produce a lignin-specific enzyme cocktail from D. squalens or other white-rot fungi.

KW - Aromatics

KW - Basidiomycete

KW - DEGRADATION

KW - Gene expression

KW - LACCASE

KW - Lignin

KW - MANGANESE PEROXIDASE

KW - White-rot

KW - 1183 Plant biology, microbiology, virology

U2 - 10.1016/j.jbiotec.2019.11.014

DO - 10.1016/j.jbiotec.2019.11.014

M3 - Article

VL - 308

SP - 35

EP - 39

JO - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

ER -